Reaction of sugarcane genotypes to brown and to orange rust by leaf whorl inoculation

Reação de genótipos de cana-de-açúcar às ferrugens marrom e alaranjada por inoculação no cartucho foliar

ABSTRACT:

In this research eleven sugarcane genotypes were classified in relation to their resistance to brown rust, and eleven to their resistance to orange rust. Artificial inoculation was carried out in the leaf whorl of 165-day-old plants in the city of Paranavaí, Paraná State, Brazil, in 2017. The evaluation was performed 30 days after inoculation, using a rating scale. Among the genotypes tested for brown rust, four were classified as susceptible, six as moderately susceptible and one presented moderate resistance. For orange rust, three genotypes were classified as susceptible, seven as moderately susceptible and one as moderately resistant. The evaluation and classification of the reaction of sugarcane genotypes to the rusts is an important tool that assist in preliminary trials and selection of promising genotypes for more advanced stages of breeding programs and provides information to producers on the choice of cultivars to be planted.

Key words:
Saccharum spp.; Puccinia melanocephala; Puccinia kuehnii; classification; resistance.

RESUMO:

Neste trabalho onze genótipos de cana-de-açúcar foram classificados em relação à sua resistência à ferrugem marrom, e onze quanto à resistência à ferrugem alaranjada. Foi realizada a inoculação no “cartucho foliar” de colmos com 165 dias, no município de Paranavaí, PR, em 2017. A avaliação foi feita aos 30 dias após a inoculação, utilizando uma escala de notas. Dos genótipos avaliados para ferrugem marrom, quatro foram classificados como suscetíveis, seis como moderadamente suscetíveis e um apresentou resistência moderada. Para a ferrugem alaranjada, três genótipos foram classificados como suscetíveis, sete como moderadamente suscetíveis e um como moderadamente resistente. A avaliação e classificação da reação de genótipos de cana-de-açúcar às ferrugens é uma ferramenta importante que auxilia em ensaios preliminares, na escolha de genótipos promissores para fases mais avançadas dos programas de melhoramento além de fornecer informações aos produtores na seleção das cultivares que serão plantadas.

Palavras-chave:
Saccharum spp.; Puccinia melanocephala; Puccinia kuehnii; classificação; resistência.

Sugarcane is an important crop in Brazil, with a production estimated in 747 million tons in the 2017/2018 harvest, which corresponds to about 39% of the world total, giving the country the title of largest world producer (FAOSTAT, 2020). Yield is influenced by factors such as crop genetic characteristics, climatic factors, soil physicochemical characteristics, nutrient availability, pests, weeds and diseases (GILBERT et al. 2006; LIMA et al. 2017).

Among the diseases, the rusts stand out. Brown rust, caused by the fungus Puccinia melanocephala Syd. & P. Syd., was first reported in Brazil in the municipality of Capivari (SP), in 1986, and spread rapidly throughout the other producing regions of the country (AMORIM et al. 1987). It promotes reduction of photosynthetic area causing delay in plant development in susceptible cultivars. Orange rust, caused by Puccinia kuehnii (W. Krüger) E. J. Butler, was first observed in Brazil in 2009, when it affected the production of three highly susceptible cultivars, SP89-1115, RB72454 and SP84-2025, corresponding to 10% of sugarcane plantations in the country at the time (BARBASSO et al. 2010). Like P. melanocephala, the fungus produces pustules on the leaf surface, reducing photosynthetic activity, production and quality of the final product (ZHAO et al. 2011).

Although, fungicide application is employed as an emergency technique, the main control method of sugarcane rusts is the use of resistant cultivars (ROTT et al. 2016). For such a control measure to be adopted efficiently, it is necessary to know the level of genotype resistance to diseases. Most of the studies on classification of Brazilian genotypes regarding their resistance to diseases are dedicated to orange rust (ARAÚJO et al. 2013; KLOSOWSKI et al. 2015; CHAPOLA et al. 2016; URASHIMA et al. 2018), with those focusing on brown rust being prior to the arrival of Puccinia kuehnii in the country (IDO et al. 2006). Thus, the objective of this study was to verify the reaction of sugarcane genotypes to brown and orange rust in terms of their resistance using leaf whorl inoculation technique.

Two experiments (one experiment for each disease) were conducted in 2016/17 for evaluation of genotypes in relation to resistance to brown rust and to orange rust in an experimental area in the municipality of Paranavaí, Paraná, Brazil (23°05’ S; 52°26’ W, 470 m asl), where a meteorological station was also installed to obtain climate data. The design used in both experiments was that of casualized blocks, with eleven treatments and four repetitions. The experimental unit consisted of two plants per genotype, spaced by 0.5 m, and 2 m between treatments.

The genotypes evaluated for brown rust were: CTC-4, RB835486, RB966229, RB036065, RB036147, RB056388, RB106803, RB106811, RB106814, RB106819, RB106822; and for orange rust were: SP81-3250, RB72454, RB006629, RB036059, RB036145, RB036153, RB036163, RB056388, RB106803, RB106819, RB106822. Genotypes with known reaction to rust were included in the experiments. For brown rust, CTC-4 were used as susceptible standard; while RB106819 were used as resistance standards. For orange rust, susceptible standard were RB72454 and RB106819 as resistant standard. The other genotypes were chosen for their genetic potential of interest for the sugarcane improvement program.

The seedlings used came from individualized buds, which were planted in tubes containing commercial substrate and filter cake in a ratio of 1:1, on October 19, 2016, and kept in a greenhouse with irrigation by sprinkling until 60 days. After this period, the seedlings were manually transplanted to the field.

To obtain spore suspension for plant inoculation, urediniospores of P. melanocephala and P. kuehnii were collected in the experimental station of UFPR in Paranavaí, PR, using a vacuum pump and a glass collector at 15 days before inoculation by aspirating the surface of symptomatic leaves from susceptible cultivars, as described by SOOD et al. (2009) and stored in a freezer (± 2 °C). The preparation of suspensions of the two rusts occurred in the same way, adding the spores in distilled water and shaking, in order to homogenize each suspension. The concentration was adjusted to 10⁴ viable spores mL^-1 using a hemacytometer (Neubauer chamber, Optik Labor, Germany) (SOOD et al. 2009), adding 0.1% of Tween 20 to the final volume of each suspension.

Inoculation was performed 105 days after transplanting the seedlings to the field, using 0.5 mL of suspension (water + spores + Tween 20), which was placed separately inside the leaf whorls of six individual stalks per replicate using a repeater pipette, being inoculated the three tallest stalks of each plant, which were identified for further evaluation by cutting 1/3 of the top of the tallest leaves.

After 30 days of incubation, the inoculated leaves were evaluated, after they emerged from the whorl, where the symptoms were demonstrated as a band of pustules (in susceptible varieties). The symptoms of rust were assessed on the 0-4 scale (SOOD et al. 2009), with 0 - no symptoms, 1 - chlorotic flecks, 2 - orange-brown lesions, without sporulation, 3 - one to five pustules with sporulation (production of urediniospores), and 4 - six or more coalescent pustules with sporulation resulting in leaf necrosis. Treatments were analyzed based on the averages obtained in the field and classified according to the modified SOOD et al. (2013) scale, in which the genotypes with 0 - 1 notes were considered resistant; 1.1 - 2, moderately resistant; 2.1 - 3, moderately susceptible and 3.1 - 4, susceptible.

The average temperature between the inoculation and plant evaluation (30 days) was 21.5 ºC, with a minimum of 15.5 ºC and a maximum of 25.7 ºC, and according to SANJEL et al. (2019) the ideal mean temperature for the development of both rusts is between 20 and 22.2 °C. Average relative humidity of air during 30 days was 80%, with accumulated precipitation of 177.6 mm.

In the inoculated stalks, the symptoms were observed in bands of pustules well defined in the youngest leaf, the one that had contact with the urediniospores and its growth occurred between the inoculation and the evaluation. Based on the evaluation method proposed by SOOD et al. (2009), of the genotypes tested for brown rust, four were classified as susceptible, six as moderately susceptible and only one showed moderate resistance. Genotypes inoculated with P. kuehnii showed a similar pattern, three of them were classified as susceptible, seven as moderately susceptible and one as moderately resistant (Table 1).

Table 1
Brown and orange rust reaction of sugarcane genotypes based on leaf whorl artificial inoculation.

-------------------------------------Brown rust-------------------------------			----------------------------------Orange rust-------------------------------
Genotype	Score*	Reaction	Genotype	Score*	Reaction
RB966229	4.00	Susceptible	RB036145	4.00	Susceptible
RB056388	3.96	Susceptible	RB106803	3.96	Susceptible
RB835486	3.75	Susceptible	SP81-3250	3.88	Susceptible
RB036065	3.25	Susceptible	RB106822	3.04	Moderately susceptible
CTC-4	3.00	Moderately susceptible	RB056388	3.00	Moderately susceptible
RB106803	3.00	Moderately susceptible	RB72454	3.00	Moderately susceptible
RB106822	3.00	Moderately susceptible	RB036163	2.96	Moderately susceptible
RB106811	2.92	Moderately susceptible	RB036153	2.83	Moderately susceptible
RB106814	2.88	Moderately susceptible	RB106819	2.83	Moderately susceptible
RB036147	2.75	Moderately susceptible	RB036059	2.75	Moderately susceptible
RB106819	2.00	Moderately resistant	RB006629	2.00	Moderately resistant

• ^*Mean score of 6 observations per treatment, according to the modified scale of SOOD et al. (2013) in which: scores of 0 - 1: resistant; 1.1 - 2: moderately resistant; 2.1 - 3: moderately susceptible and 3.1 - 4: susceptible.

In this study, it was observed that there are differences between the resistance levels of the genotypes for both brown and orange rust. SOOD et al. (2013) pointed out that the leaf whorl artificial inoculation of P. melanocephala and P. kuehnii can be performed even at seasons of the year when rust spores are not available in the field, because preserved urediniospores can be used. Because it is carried out in the field, leaf whorl inoculation may be a way to take advantage of routine trials of sugarcane breeding programs, in order to expose the genotypes to a condition favorable to the occurrence of the disease, assisting in preliminary trials and the selection of promising genotypes for more advanced phases of the programs (SOOD et al. 2009). In addition, the evaluation and classification of genotypes in relation to their resistance provides information for producers on the choice of cultivars to be planted.

ACKNOWLEDGEMENTS

This study was financed in part by the ‘Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil’ (CAPES) - Finance Code 001 and ‘Rede Interuniversitária para o Desenvolvimento Sucroalcooleiro (RIDESA/PMGCA/UFPR)’. The sixth author received research fellowship from National Council for Scientific and Technological Development (CNPq)/Brazil.

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• CR-2019-0792.R1

Publication Dates

• Publication in this collection
  18 May 2020
• Date of issue
  2020

History

• Received
  09 Oct 2019
• Accepted
  17 Feb 2020
• Reviewed
  27 Apr 2020