Dermoscopic characteristics of congenital melanocytic nevi in a cohort study in southern Brazil^☆

Dear Editor,

Congenital melanocytic nevi (CMN) are benign proliferations of melanocytes present at birth or which appear during the first two years of life.¹ They are classically classified according to their largest diameter, as small (less than 1.5cm), medium (between 1.5–20cm), and large or giant (greater than 20cm).² Larger lesions are more frequently associated with the development of melanoma and have a higher risk of extracutaneous complications (neurocutaneous melanocytosis). Regarding malignancy, prospective studies have established that the overall incidence of melanomas in CMN is low (1%-2%). However, this incidence varies greatly according to the phenotype severity.³,⁴,⁵

Digital dermoscopy is a noninvasive test. The knowledge of dermoscopic characteristics is important so that this method can be used for patient diagnosis, follow-up, and management. There are few studies evaluating the evolution of the dermoscopic pattern of these nevi over time and its association with clinical and epidemiological characteristics. There is no standardization regarding the ideal period between assessments.⁴,⁶,⁷,⁸,⁹ The aim of the present study was to assess the clinical and dermoscopic characteristics of patients with CMN, comparing the dermoscopic findings at two medical consultations.

A retrospective cohort study was carried out to analyze the medical and photographic records obtained with digital videodermoscopy (Fotofinder Systems, GmbH, Bad Birnbach, Germany) of patients with CMN referred to the Dermoscopy Sector of a reference service in southern Brazil between 2016 and 2018. Data were collected on the patients general health status, dermatological anamnesis, the evolution of the congenital lesions, and stored macroscopic and microscopic photographic records. The description of variables and possible associations between clinical and dermoscopic aspects were verified.

The results were presented as central tendency measures (mean and median) and variability (standard deviation and interquartile range), in addition to absolute and relative distribution (n, %). The symmetry of continuous distributions was evaluated using the Kolmogorov-Smirnov test; Pearson’s chi-square test (χ²) was used for the bivariate analysis of the qualitative variables.

Dermoscopic data were analyzed by two experienced evaluators and were based on the analysis of patterns classified as reticular (Fig. 1), globular (Fig. 2), homogeneous, reticular-homogeneous, globular homogeneous, reticular-globular, and acral patterns (parallel ridges or furrows, fibrillar, lattice and homogeneous). The evaluation was performed in two ways: a descriptive analysis of dermoscopic data of all lesions (those with only one record and those with follow-up; n =82) and a comparative analysis of dermoscopic data of lesions that were followed over time. (n = 70). Both authors analyzed all recorded lesions and the final diagnostic definitions were attained by consensus. McNemar’s test was used to compare the data. The present study was approved by the Research Ethics Committee of the institution.

Figure 1
Brownish macula on dermoscopy showing a typical reticular pattern.

Figure 2
Small brownish papule on dermoscopy showing a typical globular pattern.

Eighty-two CMN were observed in 72 patients. Most individuals were phototype III (62.5%), had dark brown/black eyes (67.6%); medium/dark brown hair (38.9%) and 45.1% of the lesions were located on the extremities. Most lesions were small (58.5%; Table 1). The reticular pattern was the predominant dermoscopic pattern (31.7%), with the pigment network being the most common dermoscopic structure (70.7%; Table 2). The comparison between the first and the last clinical and dermoscopic examinations included 70 lesions, with a mean interval between them of 12.49 months and a median of 10 months (1^st–3^rd quartile: 8.0–12.0), with the minimum period between the two assessments being 4 months and a maximum of 21 months. No morphological and structural differences were detected at the follow-up (p > 0.05; Table 3).

Table 1
Clinical and epidemiological characterization of patients with congenital melanocytic nevi (n = 72).

Variable	Total sample (n = 72)
	n	%
Sex
Male	30	41.7
Female	42	58.3
Age (years)
Mean ±standard deviation (range)	27.2 ± 21.9 (1.0–76)
Median (1st–3rdquartile)	16.0 (10.3–43.7)
Phototype
II	6	8.3
III	45	62.5
IV	16	22.2
V	4	5.6
VI	1	1.4
Eye color
Dark brown/black	48	67.6
Light brown/hazel	13	18.3
Light green	5	7.0
Blue	5	7.0
Skin color/ethnicity
White	63	87.5
Brown	7	9.7
Black	2	2.8
Hair color
Black	22	30.6
Dark/medium brown	28	38.9
Light brown	15	20.8
Blond	7	9.7
Sunburns
Never	33	45.8
Once or more	39	54.2
Use of sunscreen
Never	5	6.9
Occasionally	59	81.9
Always/daily	8	11.1
Family history of skin cancer
No	54	75.0
Yes	18	25.0
Number of CMN
More than one	9	12.5
One	63	87.5
Location of the main CMN
Head and neck	7	8.5
Trunk	33	40.3
Extremities	37	45.1
Gluteal region	3	3.7
Inguinal region	2	2.4
Classification of the main CMN
Small	48	58.5
Medium-sized	30	36.6
Large/Giant	4	4.9
Recent modification
No	65	90.3
Yes	7	9.7
Histopathological evaluation
No	68	94.4
Yes	4	0.6

Table 2
Dermoscopic pattern of congenital melanocytic nevi (n = 82).

Characteristics	n	Lesions assessed only once (n = 82) Before	Lesions with follow-up (n =70)
			After
		%	n	%	n	%	pb
Dermoscopic pattern
Reticular	26	31.7	23	32.9	23	32.9
Globular	14	17.1	12	17.1	12	17.1	>0.999
Reticulo-globular	12	14.6	9	12.9	9	12.9
Homogeneous	17	20.7	15	21.4	15	21.4
Reticular-homogeneous	12	14.6	11	15.7	11	15.7
Parallel ridge	1	1.2
Color
Light brown	60	73.2	54	77.1	54	77.1	0.815
Dark brown	62	75.6	54	77.1	53	75.7	0.869
Blacka	3	3.7	1	1.4	1	1.4	>0.999
Reda	4	4.9	3	4.3	3	4.3	>0.999
Bluea					1	1.4	>0.999
Whitea	3	3.7	2	2.9	2	2.9	>0.999
Blue-gray	7	8.5	6	8.6	6	8.6	>0.999
Color symmetry a					2
Yes	77	93.9	65	95.6	65	95.6	>0.999
No	5	6.1	3	4.4	3	4.4
Symmetry of structures
Yes	76	92.7	66	94.3	66	94.3	>0.999
No	6	7.3	4	5.7	4	5.7
Pigment network
Yes	58	70.7	49	70.0	49	70.0	>0.999
No	24	29.3	21	30.0	21	30.0
Dots
Yes	49	59.8	41	58.6	41	58.6	>0.999
No	33	40.2	29	41.4	29	41.4
Globules
Yes	51	62.2	45	64.3	45	64.3	>0.999
No	31	37.8	25	35.7	25	35.7
Striae
No	82	100.0	70	100.0	70	100.0	–
Irregular striae
No	82	100.0	70	100.0	70	100.0	–
Structureless areas
Yes	9	11.0	6	8.6	6	8.6	>0.999
No	73	89.0	64	91.4	64	91.4
Regression a
Yes	1	1.2	1	1.4	1	1.4	>0.999
No	81	98.8	68	98.6	68	98.6
Hyperchromic macules
Yes	3	3.7	2	2.9	2	2.9	>0.999
No	79	96.3	68	97.1	68	97.1
Pseudocysts
Yes	5	6.1	5	7.1	5	7.1	>0.999
No	77	93.9	65	92.9	65	92.9
Perifollicular hyperpigmentation a
No	82	100.0	69	100.0	69	100.0	–
Perifollicular hypopigmentation
Yes	19	23.2	19	27.1	18	25.7	0.978
No	63	76.8	51	72.9	52	74.3
Hypertrichosis
Yes	19	23.2	15	21.4	14	20.0	>0.999
No	63	76.8	55	78.6	56	80.0
Blue-gray veil
No	82	100.0	70	100.0	70	100.0	–
Vascular structures
Yes	1	1.2	1	1.4	1	1.4	>0.999
No	81	98.8	69	98.6	69	98.6
Shiny white structures a
No	82	100.0	69	100.0	69	100.0	–
Negative pigment network a
Yes	4	4.9	4	5.8	4	5.8	>0.999
No	78	95.1	65	94.2	65	94.2

• ^a
  Missing data – Black color, Red color, blue color, white color, regression, perifollicular hyperpigmentation; shiny white structures, pigment network, atypical vascular pattern (1 [1.6%]); color symmetry, globules (2 [3,2]).
• ^b
  McNemar-Bowker Test.

Table 3
Dermoscopic characteristics and follow-up interval.

Characteristics	Time of interval - months (n = 70)
	Up to 10 months(n = 47)					>10 months (n = 23)
	Before		After		pb	Before		After		pb
	n	%	n	%		n	%	n	%
Dermoscopic pattern					–				–
Reticular	15	31.9	15	31.9		8	34.8	8	34.8
Globular	9	19.1	9	19.1		3	13.0	3	13.0
Reticulo-globular	5	10.6	5	10.6		4	17.4	4	17.4
Homogeneous	8	17.0	8	17.0		7	30.4	7	30.4
Reticular-homogeneous	10	21.3	10	21.3		1	4.3	1	4.3
Homogeneous pattern										>0.999
Yes	19	40.4	19	40.4	–	8	34.8	9	39.1
No	28	59.6	28	59.6		15	65.2	14	60.9
Color symmetry a					–					–
Yes	44	93.6	43	93.5		23	100.0	22	100.0
No	3	6.4	3	6.5
Structure symmetry					–				–
Yes	45	95.7	45	95.7		21	91.3	21	91.3
No	2	4.3	2	4.3		2	8.7	2	8.7
Pigment network					–					–
Yes	34	72.3	34	72.3		15	65.2	15	65.2
No	13	27.7	13	27.7		8	34.8	8	34.8
Dots					–					–
Yes	24	51.1	24	51.1		17	73.9	17	73.9
No	23	48.9	23	48.9		6	26.1	6	26.1
Globules					–					–
Yes	30	63.8	30	63.8		15	65.2	15	65.2
No	17	36.2	17	36.2		8	34.8	8	34.8

• ^a
  Missing data – Pigment network, Color symmetry, globules (2 [3,2%]).
• ^b
  McNemar-Bowker Test.

The lesions of four patients were submitted to histopathological analysis. The indications in these cases were as follows: the presence of a central papule in a medium-sized CMN located on the right shoulder of a 10-year-old patient, the presence of a central papule in a small lesion located on the fifth left toe of a 10-year-old patient, a satellite lesion in a patient with a large CMN located on the lumbar region and a lesion with structure asymmetry and a homogeneous pattern with predominant blue-gray color, located on the left thigh of a 10-year-old patient. All histopathological diagnoses were of compound melanocytic nevi. None of the assessed patients developed melanoma. Of the four patients with large/giant CMN included in the study, three had an indication for imaging examination and two had already undergone nuclear magnetic resonance with normal results.

Some studies have sought to describe specific dermoscopic features of CMN. Light brown globules with a central dot (target globules), target pigment network, focal pigment network thickening, perifollicular/focal hypopig-mentation, and target vessels have been described as characteristics present in congenital lesions, although they were not specific. In the case of lesions that must be monitored over time, digital dermoscopy appears as an important tool. There are only a few studies describing the evolution and comparison of the dermoscopic features of these lesions and their correlations with the histopathological diagnoses.¹⁰ Regarding the clinical data of patients in the present study, it was observed that small nevi predominated, a result consistent with other published data.⁸,⁹ The predominant dermoscopic pattern was the reticular one, regardless of the nevus location, unlike the data found in the literature, which found the globular pattern to be the most common. When comparing the predominant dermoscopic pattern with age, it was observed that the globular pattern was related to patients younger than 12 years, whereas the reticular pattern was related to those over this age, corroborating the data found in the literature.¹⁰,¹¹

A recent study found characteristics of atypical nevi present in CMN, such as radiating striae, focal hypopig-mentation, atypical globules and blotches, regression, and blue-gray veil. In the present study, the authors did not find striae nor blue-gray veils; the vast majority of globules were typical, and regression was observed in only one lesion.¹⁰

In addition to the difficulty in the follow-up of patients, there is difficulty regarding the long follow-up time, necessary for any changes to appear. The present study sought to evaluate the dermoscopic characteristics and their changes during videodermoscopy follow-up and correlate them to biopsy indications and histopathological results. Using descriptive data, the clinical and dermoscopic characteristics of the CMN in the initial evaluation of the patients were demonstrated.

When comparing the images recorded in the initial and final evaluations, it was observed that there were no significant differences in the studied period. One of the limitations is the reduced follow-up time (mean of 12 months) since many of the expected outcomes (clinical and dermoscopic changes that indicate lesion excision, and the infrequent presence of malignancy) generally develop after many years. Another limitation is the diversity of the studied population, including children and adults, considering that lesions tend to be stable in the latter. The relatively small number of patients (72) is also be one of the limiting factors.

The present study obtained results that allow the clinical and dermoscopic characterization of patients with CMN, although studies with a larger sample size and a longer follow-up interval are necessary to demonstrate the real benefit of digital dermoscopy when monitoring these lesions over time.

• Financial support
  None declared.
• ☆
  Study conducted at the Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.

References

• ¹ Caccavale S, Calabrese G, Mattiello E, Broganelli P, Ramondetta A, Pieretti G, et al. Cutaneous melanoma arising in congenital melanocytic nevus: a retrospective observational study. Dermatology. 2020;14:1–6.
• ² Kopf AW, Bart RS, Hennessey P. Congenital nevocytic nevi and-malignant melanomas. J Am Acad Dermatol. 1979;1:123–30.
• ³ Wu PA, Mancini AJ, Marghoob AA, Frieden IJ. Simultaneous occurrence of infantile hemangioma and congenital melanocytic nevus: coincidence or real association? J Am Acad Dermatol. 2008;58 Suppl:S16–22.
• ⁴ Tannous ZS, Mihm Jr MC, Sober AJ, Duncan LM. Congenital melanocytic nevi: clinical and histopathologic features, risk of melanoma, and clinical management. J Am Acad Dermatol. 2005;52:197–203.
• ⁵ Zaal LH, Mooi WJ, Klip H, Horst CMAM. Risk of malignant transformation of congenital melanocytic nevi: a retrospective nationwide study from The Netherlands. Plast Reconstr Surg. 2005;116:1902–9.
• ⁶ Sahin S, Levin L, Kopf AW, Rao BK, Triola M, Koenig K, et al. Risk of melanoma in mediumsized congenital melanocytic nevi: a follow-up study. J Am Acad Dermatol. 1998;39: 428–33.
• ⁷ Seidenari S, Martella A, Pellacani G. Polarized light-surface microscopy for description and classification of small and mediumsized congenital melanocytic naevi. Acta Derm Venereol. 2003;83:1–6.
• ⁸ Fernandes NC, Machado JLR. Estudo clínico dos nevos melanocíticos congênitos na criança e no adolescente. An Bras Dermatol. 2009;84:129–35.
• ⁹ Cengiz FP, Emiroglu N, Ozkaya DB, Su O, Onsun N. Dermoscopic features of small, medium, and large-sized congenital melanocytic nevi. Ann Dermatol. 2017;29:26–32.
• ¹⁰ Changchien L, Dusza SW, Agero ALC, Korzenko AJ, Braun RP, Sachs D, et al. Age - and site-specific variation in the dermoscopic patterns of congenital melanocytic nevi an aid to accurate classification and assessment of melanocytic nevi. Arch Dermatol. 2007;143:1007–14.
• ¹¹ Stefanaki C, Soura E, Stergiopoulou A, Kontochristopoulos G, Katsarou A, Potouridou I, et al. Clinical and dermoscopic characteristics of congenital melanocytic naevi. J Eur Acad Dermatol Venereol. 2018;32:1674–80.

Publication Dates

• Publication in this collection
  30 Sept 2022
• Date of issue
  Sep-Oct 2022

History

• Received
  25 Nov 2020
• Accepted
  20 Mar 2021
• Published
  22 July 2022