Water potential, biochemical indicators and yield of sugarcane irrigated with brackish water and leaching

Potencial hídrico, indicadores bioquímicos e produtividade da cana-de-açúcar irrigada com águas salobras e lixiviação

ABSTRACT

Knowledge on the water relations and biochemical responses of plants has been pointed out as important for selecting genotypes that are tolerant to abiotic stresses, such as drought and salinity. Thus, the objective of this study was to evaluate the leaf water potential, enzymatic activity and yield of sugarcane (RB 92579) irrigated with brackish waters and leaching fractions. The study was conducted from November 2016 to November 2017 in Recife, PE, Brazil. The experimental design was completely randomized, in a 5 x 2 factorial scheme, with four repetitions. Treatments consisted of five irrigation water salinity (0.5; 2.0; 4.0; 6.0 and 8.0 dS m^-1) and two leaching fractions (0 and 0.17), corresponding to 100 and 120% of crop evapotranspiration. The values of irrigation water salinity were obtained by adding NaCl and CaCl₂ at molar proportion of 1:1 (Ca:Na) to water from the local supply (ECw = 0.5 dS m^-1). Readings of leaf water potential (Ψw) were taken at predawn, and +3 leaves were collected for the determination of catalase and ascorbate peroxidase activity at 90, 150, 210 and 270 days after planting. Yield was determined at 365 days after planting. Increase in water salinity reduced leaf water potential and yield, and increased the activity of catalase and ascorbate peroxidase, and the leaching fraction of 0.17 was able to reduce the deleterious effects of salts on plants.

Key words: Saccharum spp.; oxidative stress; salinity; leaching fraction

RESUMO

O conhecimento das relações hídricas e das respostas bioquímicas de plantas tem sido apontado como importante para a seleção de genótipos tolerantes aos estresses abióticos, tais como seca e salinidade. Assim, objetivou-se avaliar o potencial hídrico foliar, atividade enzimática e produtividade da cana-de-açúcar (RB 92579) irrigada com águas salobras e frações de lixiviação. A pesquisa foi conduzida no período de novembro 2016 a novembro 2017 em Recife, PE, Brasil. Adotou-se o delineamento experimental inteiramente casualizado, em esquema fatorial 5 x 2, com quatro repetições. Os tratamentos consistiram de cinco salinidades da água de irrigação (0,5; 2,0; 4,0; 6,0 e 8,0 dS m^-1) e duas frações de lixiviação (0 e 0,17), correspondendo a 100 e 120% da evapotranspiração da cultura. Os valores de salinidade da água de irrigação foram obtidos pela adição de NaCl e CaCl₂ na proporção de 1:1 molar (Ca:Na) na água de abastecimento (CEa = 0,5 dS m^-1). Foram realizadas leituras do potencial hídrico foliar (Ψw) no antemanhã e coletadas folhas +3 para a determinação da atividade da catalase e da ascorbato peroxidase aos 90, 150, 210 e 270 dias após o plantio. A produtividade foi determinada aos 365 dias após o plantio. O aumento da salinidade da água reduziu o potencial hídrico foliar e o rendimento, e, aumentou a atividade da catalase e ascorbato peroxidase, sendo a fração de lixiviação de 0,17 capaz de reduzir os efeitos deletérios dos sais sobre as plantas.

Palavras-chave: Saccharum spp.; estresse oxidativo; salinidade; fração de lixiviação

Introduction

Brazil is the world’s largest producer of sugarcane, one of the most efficient bioenergy sources. In the Northeast region, crops are concentrated on the coast (CONAB, 2018), where the presence of brackish waters due to saline intrusion is common. Thus, the use of brackish waters and/or inadequate management of the soil-water-plant system can cause damage to plant physiology and yield in irrigated areas (Munns & Tester, 2008; Herbert et al., 2015).

Salt accumulation reduces the osmotic potential of the soil solution and water availability, and the specific ionic effects resulting from the absorption of toxic ions (Na⁺ and Cl^-) interfere in the absorption of nutrients and cause cytotoxicity (Mansour, 2014).

In order to adapt to the excess of salts in the root environment, plants reduce leaf water potential to maintain a favorable gradient for water absorption and flow in the soil-root-shoot system, a process that most often increases metabolic energy expenditures (Negrão et al., 2017; Taiz et al., 2017).

Salt stress, especially at moderate to severe levels, causes a secondary stress called oxidative stress, capable of generating accumulation of reactive oxygen species (ROS) and damage to membranes and macromolecules. At the cellular level, however, plants have developed mechanisms capable of neutralizing the damage caused by ROS (Carvalho et al., 2016). Among these mechanisms, antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD) stand out (Anitha et al., 2015; Ferreira et al., 2017).

Considering that the literature is scarce with respect to studies on sugarcane crop under salt stress (Endres et al., 2010; Anitha et al., 2015; Carvalho et al., 2016), the objective was to evaluate the leaf water status, activity of enzymes of the antioxidative protection system and yield along the cultivation cycle of sugarcane (RB 92579) irrigated with brackish waters and leaching fractions.

Material and Methods

The experiment was conducted at the Prof. Ronaldo Freire de Moura Lysimetric Station of Irrigated Agriculture, located at the Department of Agricultural Engineering (DEAGRI) of the Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil, whose geographical coordinates are 8° 1’ 6” S and 34° 56’ 49” W, and altitude of 6 m.

The total area used has dimensions of 32 x 75 m (2400 m²), being located in the central part of a lysimetric station (18 x 25.80 m) composed of 40 drainage lysimeters (1 m³) installed equidistantly at 1.20 m, in both directions, and an automatic meteorological station (Campbell Scientific - CR1000 model) used to obtain climatic data for irrigation management.

The lysimeters were filled with soil (0-0.40 m layer) from the municipality of Goiana, PE, Brazil, classified as a Spodosol Orthod in the classification of U.S. Soil Taxonomy (Soil Survey Staff, 2014). Its physical-chemical characteristics are: sand, silt and clay = 890, 30 and 80 g kg^-1, respectively; textural class = sand; soil density = 1.73 kg dm^-3; particle density = 2.63 kg dm^-3; volumetric moisture (0.33 atm) = 3.02%; volumetric moisture (15 atm) = 1.34%; organic matter = 15.35 g kg^-1; pH_(H2O) = 6.5; P = 49 mg dm^-3; K⁺ = 0.08 cmol cm^-3; Ca⁺ = 1.6 cmol_c dm^-3; Mg²⁺ = 0.65 cmol_c dm^-3; Na⁺ = 0.06 cmol_c dm^-3; H⁺+Al³⁺ = 3.05 cmol_c dm^-3; Al³⁺ = 0 cmol_c dm^-3; cation exchange capacity = 5.4 cmol_c dm^-3; base saturation = 44%; and aluminum saturation = 0%.

According to the recommendation of fertilization for the state of Pernambuco, Brazil (Cavalcanti et al., 2008), basal mineral fertilization was performed with 20 kg ha^-1 of N, 40 kg ha^-1 of P₂O₅ and 35 kg ha^-1 of K₂O. At 45 and 150 days after planting (DAP), topdressing fertilization was performed applying 20 kg ha^-1 of N and 35 kg ha^-1 of K₂O. Application of micronutrients was performed through leaf fertilization, applying 1.3 kg ha^-1 of Cu, 2.0 kg ha^-1 of Zn and 2.6 kg ha^-1 of Mn.

The sugarcane variety RB 92579 was planted on November 19, 2016, using six cuttings per linear meter, with two buds each, and performing a thinning after emergence to keep 8 plants m^-1.

Treatments consisted of the combination of five irrigation water salinity (ECw = 0.5; 2.0; 4.0; 6.0 and 8.0 dS m^-1) and the conditions without (LF1 = 0) and with leaching fraction (LF2 = 0.17), corresponding to 100 and 120% crop evapotranspiration (ETc). The experimental design used was completely randomized in 5 x 2 factorial scheme, with four repetitions.

Water salinity values were obtained by adding NaCl and CaCl₂.H₂O (A.R.) at molar proportion of 1:1 (Ca:Na) to the local supply water (ECw = 0.5 dS m^-1), at concentrations necessary to obtain the pre-established values of electrical conductivity, according to Richards (1954), through Eqs. 1 and 2:

$$Q=640\cdot ECw\text{when}ECw<5.0{\text{dS m}}^{-1}$$ (1)

$$Q=800\cdot ECw\text{when}ECw>5.0{\text{dS m}}^{-1}$$ (2)

where:

Q - quantity of salts, mg L^-1; and,

ECw - desired value of water electrical conductivity, dS m^-1.

Sodium and calcium chloride salts are some of the most frequently found salts in the irrigation water of the Northeast region, so the leaching fraction (0.17) associated with the drainage system were used as a strategy to mitigate the effects of salts on plants.

Treatments began to be applied at 60 DAP, with daily irrigations using the respective saline waters based on ETc, obtained by the product of reference evapotranspiration (ETo) and crop coefficient (Kc). ETo was estimated by the Penman-Monteith equation (Allen et al., 1998). The Kc used corresponds to the phenological stage of the plant, according to the United Nations Food and Agriculture Organization - FAO (Allen et al., 1998). Irrigation was applied by a drip system with pressure-compensating emitters, spaced at 0.30 m and with unit flow rate of 4.1 L h^-1.

The water depth received through rainfall over the period was 1774 mm, but with water surplus in only one month in relation to crop demand (Figure 1). The reference evapotranspiration accumulated throughout the cultivation cycle was 1287.35 mm (3.52 mm d^-1). The irrigation depths applied based on 100 and 120% ETc, corresponding to the leaching fractions LF1 = 0 and LF2 = 0.17, were 522.53 and 609.01 mm, respectively.

Figure 1
Rainfall, reference evapotranspiration and crop evapotranspiration for 10-day periods throughout the cultivation cycle

At 90, 150, 210 and 270 days after planting - DAP, leaves were collected for analysis of leaf water potential (Ψw) by means of a Scholander-type pressure chamber (M1505D model, PMS Company). Readings were taken at predawn (before sunrise), by collecting one leaf per lysimeter, +3 leaf, which is the third visible leaf of the sheath known as TVD (Top Visible Dewlap) leaf, according to Kuijper (Dillewijn, 1952).

At 90, 150, 210 and 270 DAP, leaves (+3 leaf) were collected from the respective treatments to determine the activity of catalase (CAT) and ascorbate peroxidase (APX). These leaves were cut at the base, wrapped in aluminum foil, immersed in liquid nitrogen and immediately stored in ultra-freezer (-80 ºC) until the analytical procedure.

To obtain the enzymatic extract, 0.1 g of fresh matter was macerated in liquid nitrogen and received 4 mL of potassium phosphate buffer (50 mM), with pH 7.0 and Polyvinylpyrrolidone (PVP) to avoid oxidation of enzymes during the process. After maceration, the concentrate was filtered into 2-mL Eppendorf tubes and placed in refrigerated centrifuge for 10 min at 10,000 rpm (4 ºC). Catalase activity was determined using the methodology proposed by Kar & Mishra (1976), while ascorbate peroxidase was determined following the methodology of Nakano & Asada (1981).

At 365 DAP, harvest was carried out and the stems were weighed to obtain the fresh biomass (TCH, Mg ha^-1).

The data were subjected to normality test, homoscedasticity test and analysis of variance by F test (p ≤ 0.05) for each evaluation period. The values of salinity were compared through regression analysis and the leaching fractions were compared by Tukey test (p ≤ 0.05), using the statistical software SISVAR (Ferreira, 2011).

Results and Discussion

Figure 2 shows that the interaction between irrigation water electrical conductivity and irrigation depths had significant effects on the leaf water potential (Ψw) of plants. At 90 (Figure 2A), 150 (Figure 2B), 210 (Figure 2C) and 270 DAP (Figure 2D), there was a linear reduction (p ≤ 0.01) of Ψw as a function of increasing salinity in the treatment without leaching fraction (LF1 = 0). For the treatment with leaching fraction (LF2 = 0.17), the response was quadratic in the first three periods, with the values remaining higher than those of plants cultivated without leaching fraction (LF1) at the highest salinity levels.

Figure 2
Leaf water potential of sugarcane (RB 92579), subjected to water salinity (ECw) at 90 (A), 150 (B), 210 (C) and 270 days after planting (D), without (LF1) and with (LF2) leaching fraction

At 90 DAP (Figure 2A), the mean values of Ψw observed for salinity levels of 0.5 and 8.0 dS m^-1 were -0.53 and -1.10 MPa, respectively, a percentage reduction of 109.2. In the treatment with leaching fraction of 0.17, corresponding to the water depth of 120% ETc, there were mean values of -0.60 and -0.78 MPa in the interval of irrigation water salinity between 0.5 and 5.22 dS m^-1, respectively, a reduction of 29.26%, remaining with values equal to or higher than -0.72 MPa at the highest levels of salinity. These results showed the efficiency of the leaching fraction in controlling salts in the soil, for the displacement of salts from irrigation water and/or those already existing in the soil to deeper areas than the root zone (Puga et al., 2016).

At 150 (Figure 2B) and 210 DAP (Figure 2C), there was a similar response to that observed at 90 DAP, but a 150 DAP the mean values of leaf water potential are probably due to the occurrence of rains in the period (Figure 1), which leached much of the salts from the root zone. This situation is consistent with the claim of Cavalcante et al. (2018), who concluded that the waters of the rainy season promoted the leaching of salts from the root environment of plants. The mean values observed for the treatment without leaching fraction at salinity levels of 0.5 and 8.0 dS m^-1 were -0.47 and -0.78 MPa, respectively, with a reduction of 66.38%.

High salt concentrations at the root-soil interface reduce water absorption by the plant, because of the decrease in soil water potential, due to both osmotic and matric effects (Oliveira et al., 2018). In addition, the absorption of salts (Na⁺ and Cl^-) in excess induces toxicity due to the specific ionic effect, compromising the absorption of nutrients and photosynthesis (Munns & Tester, 2008; Mansour, 2014).

Variations in Ψw indicate that the imposed level of salt stress varied not only with the water salinity and leaching fraction, but also with environmental conditions, especially with the occurrence of rains. This finding reinforces the importance of field trials, especially with perennial crops or crops with long cycles, such as sugarcane.

Studies conducted with well hydrated herbaceous plants have shown that Ψw ranges from -0.2 to close to -0.9 MPa, and lower values indicate that plants are under moderate water stress (Taiz et al., 2017).

Endres et al. (2010), evaluating the sugarcane variety RB 92579 under water deficit in Coruripe, AL, Brazil, observed Ψw values of -0.9 MP to (moderate drought) and up to -1.5 MPa (severe water deficit). The results of Ψw show that the stress levels experienced by sugarcane were not severe, even at the highest levels of irrigation water salinity, and were lessened by the occurrence of rains and by the use of a leaching fraction of 0.17.

At 270 DAP (Figure 2D), Ψw decreased linearly (p ≤ 0.01) for both leaching fractions (100 and 120% ETc) with the increase in water salinity. In the treatment without leaching fraction, mean values of -0.51 and -0.89 MPa were recorded at 0.5 and 8.0 dS m^-1, a percentage reduction of 74.74%. Lira et al. (2018a) observed reductions of up to 66.28% in the Ψw of sugarcane (RB 867515) subjected to ECw of 6.5 dS m^-1 without leaching fraction, at 318 DAP, in Recife, PE, Brazil.

The increase in water salinity increased the activity of catalase - CAT (p ≤ 0.01) up to the highest values of 602.81 and 613.64 μmol H₂O₂ mg^-1 protein min^-1 in plants irrigated with 6.34 dS m^-1 water at 150 (Figure 3A) and 7.01 dS m^-1 water at 210 DAP (Figure 3B), respectively. At 90 DAP, there was no significant difference (p > 0.05) between treatments.

Figure 3
Catalase activity in sugarcane (RB 92579) subjected to water salinity (ECw) at 150 (A), 210 (B) and 270 days after planting (C), without (LF1) and with (LF2) leaching fraction

These values indicate the saturation of the enzyme, evidencing that the response of this defense mechanism to oxidative stress is dependent on the level of salt stress imposed on the sugarcane crop. The results also indicate that, despite the modest decrease at the highest level of stress, the values exceed those observed in plants subjected to the lowest level of irrigation water salinity. According to Azevedo et al. (2011), the capacity of activation of the antioxidant system is one of the main mechanisms of adjustment of the plant to oxidative stress.

Figure 3C shows the effect of the interaction between irrigation water electrical conductivity and leaching fractions for catalase (at 270 DAP), with significant effect (p ≤ 0.01) for the two leaching fractions (0 and 0.17). The mean values of catalase in plants irrigated with 100% ETc water depth were 436.70 and 830.98 μmol H₂O₂ mg^-1 protein min^-1 relative to the waters with electrical conductivities of 0.5 and 6.07 dS m^-1, respectively, promoting an increase of 90.29%.

For the 120% ETc water depth, the maximum value (676.82 μmol H₂O₂ mg^-1 protein min^-1) was only observed at salinity of 7.07 dS m^-1, but the values of catalase activity were significantly lower than those determined in plants irrigated with water depth of 100% ETc, regardless of water salinity level. These results indicate a lower degree of salinity-induced oxidative stress under conditions of use of leaching fraction.

Figure 3A shows the mean values of catalase activity in sugarcane leaves (150 DAP) in response to the irrigation depths (LF1 and LF2). There was a significant increase (p ≤0.01) of 21.24% in catalase due to the pronounced effect of salt stress, with values of 583.02 and 459.18 μmol H₂O₂ mg^-1 protein min^-1 corresponding to leaching fractions of 0 and 0.17, respectively. Similar results were obtained at 210 DAP (Figure 3B). The leaching fraction promoted greater removal of salts from the root system zone of the plants, reducing the intensity of salt stress.

Despite having low affinity for H₂O₂ when compared to APX, CAT has a very fast rate of peroxide degradation, being an important line of oxidative defense in plants under stress (Zhang et al., 2016; Fahad et al., 2017). This situation justifies the higher activity of the catalase enzyme in plants subjected to salt stress without leaching fraction in the present study.

The effect of interaction between irrigation water electrical conductivity and leaching conditions on the activity of ascorbate peroxidase (APX) can be observed at 150 (Figure 4A), 210 (Figure 4B) and 270 DAP (Figure 4C), with a significant quadratic effect (p ≤ 0.01). There was no significant difference (p > 0.05) between treatments at 90 DAP.

Figure 4
Ascorbate peroxidase in sugarcane (RB92579) subjected to water salinity (ECw) at 150 (A), 210 (B) and 270 days after planting (C), without (LF1) and with (LF2) leaching fraction

At 150 DAP, without leaching fraction (Figure 4A), the maximum value (34054.09 μmol H₂O₂ mg^-1 protein min^-1) was found at salinity of 7.27 dS m^-1, an increase of 95.87% compared to the lowest level of salinity (0.5 dS m^-1). With the leaching fraction of 0.17, there was a percentage increase in APX of 77.65 (6.61 dS m^-1), demonstrating the efficiency of the leaching fraction (LF2) in alleviating the effects of salt stress.

In the three periods of evaluation (Figures 4A, B and C), the behavior of APX activity is similar to that of catalase activity (Figure 3), exhibiting saturation of the response with the increase of salinity; however, APX activities were much higher than CAT activities in all periods and in all treatments.

Unlike SOD and CAT, ascorbate peroxidases use ascorbate (ASA) as an electron donor to convert H₂O₂ into H₂O and O₂ in several cell compartments such as chloroplasts, mitochondria, apoplast and cytosol (Nishiyama & Murata, 2014). Also according to these authors, the APXs have a high affinity for peroxide, identifying it even at very low concentrations (μM), unlike CATs, which can only identify it at higher levels (mM).

APX and CAT enzymes have the level of activity stabilized in plants irrigated with waters of higher salinity levels, but the values remain much higher than those observed in lower salinity treatments. This indicates that there is good potential for enzymes to degrade ROS and, consequently, mitigate the impacts of oxidative stress on cellular structures and functions. This does not mean, however, repairing all damage caused by salt stress, since oxidative degradation is only a secondary stress component associated with stress caused by excess of salts in the soil.

The results corroborate those of Carvalho et al. (2016) for the varieties RB 92579 and RB867515 subjected to salt stress with NaCl in the initial stage of growth, as these authors observed stabilization of enzymatic activity when water salinity reached values close to 6.0 dS m^-1.

APX activity was lower in plants treated with leaching fraction (LF2), for all salinity levels and in all periods of evaluation (Figure 4). According to Anitha et al. (2015), the responses of plants to salt stress are complex, occurring in genetic networks and metabolic processes that depend on the inherent salt tolerance of the species, salt concentration and duration of exposure.

For TCH (Figure 5), the unit increase in salinity levels promoted reductions of 5.70 and 4.77 Mg ha^-1 of stem fresh matter for the conditions without (LF1) and with (LF2) leaching fraction, respectively. The highest stem yield was obtained when LF2 was adopted at the salinity of 0.5 dS m^-1 (168.97 Mg ha^-1), while at the salinity level of 8.0 dS m^-1 for this same condition, TCH was equal to 133.19 Mg ha^-1 (-21.17%). In the condition of LF1, the obtained values of TCH were 130.76 and 88.01 Mg ha^-1, for the respective levels of irrigation water salinity, a reduction of -32.69%.

Figure 5
Production of green mass of stems (TCH) of sugarcane (RB92579) subjected to water salinity and without (LF1) and with (LF2) leaching fraction

According to the classification proposed by Maas & Hoffmam (1977), sugarcane has a threshold salinity of 1.7 dS m^-1, being classified as moderately sensitive to salinity. However, based on the reduction in stem yield, the variety RB 92579 was classified as moderately tolerant under both leaching conditions (LF1 and LF2), according to the classification of Fageria (1985).

Lira et al. (2018b) conducted a study in Recife, PE, Brazil, with the variety RB867515 under irrigation with brackish waters (0.5 to 6.5 dS m^-1), and observed a reduction of 28.64% in fresh matter yield, and the highest yield (116.60 Mg ha^-1) was obtained at the lowest level of salinity.

Conclusions

1. Increasing water salinity from 0.5 dS m^-1 reduces leaf water potential and stem fresh matter yield.

2. The activity of the enzymes (catalase and ascorbate peroxidase) increased with the increment in water salinity levels at all ages of the plants, with higher values in plants irrigated using water with salinity from 6.07 to 7.27 dS m^-1.

3. The leaching fraction of 0.17 increases the water status of plants and reduces the activity of antioxidant enzymes.

Acknowledgments

The work was carried out with the support of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Financing Code 001. And the Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE) with the granting of the postgraduate scholarship and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/INCTsal) for financial support.

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Publication Dates

• Publication in this collection
  29 May 2020
• Date of issue
  May 2020

History

• Received
  24 Jan 2019
• Accepted
  04 Mar 2020
• Published
  30 Mar 2020