Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e mosaico amarelo do feijoeiro

Camargo, I. J. B. de; Kitajima, E. W.; Costa, A. S.

doi:10.1590/S0006-87051968000200015

Resumos

Exames ao microscópio electrônico de tecidos foliares e radiculares de plantas infetadas pelo vírus do mosaico comum ou do mosaico amarelo do feijoeiro, mostraram a presença de dois tipos de inclusões no cito-plasma: filamentosas, consideradas como partículas de vírus, e lamelares, típicas dos vírus do grupo Y. Essas inclusões não foram encontradas no pólen ou no óvulo de feijoeiros infetados. Como o vírus do mosaico comum do feijoeiro é transmitido pelo pólen, sugere-se que êle ocorre nestas células em concentração muito baixa, ou mesmo na forma de ácido nucléico.

Two types of cytoplasmic inclusions were observed in leaf and root tissues of host plants infected with the common and yellow bean mosaic viruses: (1) filamentous inclusions considered as an aggregate of virus particles and (2) lamellar inclusions which appeared with varied configurations that represent sections at different angles of the same cylindrical structure. No type of inclusion or virus particle was seen in pollen and ovule from bean plants infected with each of the two viruses. Since, however, the common bean mosaic virus is transmitted through the pollen it is suggested that it occurs in very low concentration in this structure or else as viral nucleic acid.

Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e mosaico amarelo do feijoeiro¹ 1 Trabalho parcialmente auxiliado pelo CNPq (TC 4827) e pela FAPESP (C. Agron. 66/107).

Electron microscopy of common and yellow bean mosaic viruses in infected tissues

I. J. B. de Camargo^I,² 2 Bolsista de aperfeiçoamento da FAPESP. ; E. W. Kitajima^II; A. S. Costa^II

^IBiologista

^IIEngenheiros-agrônomos, Seção de Virologia, Instituto Agronômico

SINOPSE

Exames ao microscópio electrônico de tecidos foliares e radiculares de plantas infetadas pelo vírus do mosaico comum ou do mosaico amarelo do feijoeiro, mostraram a presença de dois tipos de inclusões no cito-plasma: filamentosas, consideradas como partículas de vírus, e lamelares, típicas dos vírus do grupo Y. Essas inclusões não foram encontradas no pólen ou no óvulo de feijoeiros infetados. Como o vírus do mosaico comum do feijoeiro é transmitido pelo pólen, sugere-se que êle ocorre nestas células em concentração muito baixa, ou mesmo na forma de ácido nucléico.

SUMMARY

Two types of cytoplasmic inclusions were observed in leaf and root tissues of host plants infected with the common and yellow bean mosaic viruses: (1) filamentous inclusions considered as an aggregate of virus particles and (2) lamellar inclusions which appeared with varied configurations that represent sections at different angles of the same cylindrical structure.

No type of inclusion or virus particle was seen in pollen and ovule from bean plants infected with each of the two viruses. Since, however, the common bean mosaic virus is transmitted through the pollen it is suggested that it occurs in very low concentration in this structure or else as viral nucleic acid.

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LITERATURA CITADA

Recebido para publicação em 20 de novembro de 1967.

1. BEEMSTER, A. B. R. & VAN DER WANT, J. P. H. Serological investigations on the Phaseolus vulgaris 1 and 2. Antonie van Leeuwenhoek, 17:285-296, 1951.
2. BERCKS, R. Serologische Untersuchungen zur differenzierung von Isolaten des Phaseolus-Virus 2 und ibrer Verwandschaft mit Phaseolus-Virus 1. Phytopath. Z. 39:120-128, 1960.
3. BRANDES, J. Identifizierung von gestreckten pflanzenpathogen Viren auf morphologischer Grundlage. Mitt. biol. BundAnst. Ld.- u. Forstw., Berlin 110:1-130, 1964.
4. ________ & QUANTZ, L. Elektronenmikroskopische Untersuchungen über Buschbohnenviren (Phaseolus-Virus l und Phaseolus Virus 2). Naturwissenschaften 42:588, 1955.
5. COSTA, A. S. Comunicaçăo pessoal.
6. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883, 1966.
7. EDWARDSON, J. R. Electron microscopy of cytoplasmic inclusions in cells infected with rod-shaped viruses. Am. J. Bot. 53:359-364, 1966.
8. HAYASHI, T.; MATSUI, C. & YAMAGUCHI, A. Electron microscopy of intra-cellular turnip mosaic virus. Phytopathology 55:458, 1965.
9. KITAJIMA, E. W. A rapid method to detect particles of some spherical plant viruses in fresh preparations. J. Electromi-croscopy, Tokyo 14:119-121, 1965.
10. LUFT, J. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.
11. MCWHORTER, F. P. Isometric crystals produced by Pisum virus 2 and Phaseolus virus 2. Phytopathology 31:760-761, 1941.
12. MATSUI, C. & YAMAGUCHI, A. Electron microscopy of host cells infected with tobacco etch virus. I. Fine structures of leaf cells at later stages of infection. Virology 22:40-47, 1964.
13. MILLONING, G. Studio sui fattori che determinano la preservazione della ultrastructtura. In: From molecule to cell. Symposium on Electron Microscopy. P. Buffa, ed. Roma, 1964. p. 347-362.
14. REDDICH, D. La transmission du virus de la mosaique du hariot par le pollen. Extr. du Deux. Cong. Inst. Path. Comp. 1931. p. 363-366.
15. ________& STEWART, B. V. Transmission of the virus of bean mosaic in seed and observations on thermal death-point of seed and virus. Phytopathology 9:445, 1919.
16. REYNOLDS, E. S. The use of lead citrate, at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:208-211, 1963.
17. SABATINI, D. D.; BENSCH, K. & BARNETT, R. J. Cytochemistry and electron microscopy. The preservation of cellular ultra-structure and enzimatic activity by aldehyde fixative. J. Cell Biol. 17:19-58, 1963.
18. WEINTRAUB, M. & RAGETLI, H. W. J. Fine structure of inclusions and organelles in Vicia fabainfected with bean yellow mosaic virus. Virology 28:390-402, 1966.

1

Trabalho parcialmente auxiliado pelo CNPq (TC 4827) e pela FAPESP (C. Agron. 66/107).

2

Bolsista de aperfeiçoamento da FAPESP.

Datas de Publicação

Publicação nesta coleção
19 Mar 2009
Data do Fascículo
1968

Histórico

Recebido
20 Nov 1967

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BEEMSTER, A. B. R. & VAN DER WANT, J. P. H. Serological investigations on the Phaseolus vulgaris 1 and 2. Antonie van Leeuwenhoek, 17:285-296, 1951.

[2] 2. BERCKS, R. Serologische Untersuchungen zur differenzierung von Isolaten des Phaseolus-Virus 2 und ibrer Verwandschaft mit Phaseolus-Virus 1. Phytopath. Z. 39:120-128, 1960.

[3] 3. BRANDES, J. Identifizierung von gestreckten pflanzenpathogen Viren auf morphologischer Grundlage. Mitt. biol. BundAnst. Ld.- u. Forstw., Berlin 110:1-130, 1964.

[4] 4. ________ & QUANTZ, L. Elektronenmikroskopische Untersuchungen über Buschbohnenviren (Phaseolus-Virus l und Phaseolus Virus 2). Naturwissenschaften 42:588, 1955.

[5] 5. COSTA, A. S. Comunicaçăo pessoal.

[6] 6. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883, 1966.

[7] 7. EDWARDSON, J. R. Electron microscopy of cytoplasmic inclusions in cells infected with rod-shaped viruses. Am. J. Bot. 53:359-364, 1966.

[8] 8. HAYASHI, T.; MATSUI, C. & YAMAGUCHI, A. Electron microscopy of intra-cellular turnip mosaic virus. Phytopathology 55:458, 1965.

[9] 9. KITAJIMA, E. W. A rapid method to detect particles of some spherical plant viruses in fresh preparations. J. Electromi-croscopy, Tokyo 14:119-121, 1965.

[10] 10. LUFT, J. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.

[11] 11. MCWHORTER, F. P. Isometric crystals produced by Pisum virus 2 and Phaseolus virus 2. Phytopathology 31:760-761, 1941.

[12] 12. MATSUI, C. & YAMAGUCHI, A. Electron microscopy of host cells infected with tobacco etch virus. I. Fine structures of leaf cells at later stages of infection. Virology 22:40-47, 1964.

[13] 13. MILLONING, G. Studio sui fattori che determinano la preservazione della ultrastructtura. In: From molecule to cell. Symposium on Electron Microscopy. P. Buffa, ed. Roma, 1964. p. 347-362.

[14] 14. REDDICH, D. La transmission du virus de la mosaique du hariot par le pollen. Extr. du Deux. Cong. Inst. Path. Comp. 1931. p. 363-366.

[15] 15. ________& STEWART, B. V. Transmission of the virus of bean mosaic in seed and observations on thermal death-point of seed and virus. Phytopathology 9:445, 1919.

[16] 16. REYNOLDS, E. S. The use of lead citrate, at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:208-211, 1963.

[17] 17. SABATINI, D. D.; BENSCH, K. & BARNETT, R. J. Cytochemistry and electron microscopy. The preservation of cellular ultra-structure and enzimatic activity by aldehyde fixative. J. Cell Biol. 17:19-58, 1963.

[18] 18. WEINTRAUB, M. & RAGETLI, H. W. J. Fine structure of inclusions and organelles in Vicia fabainfected with bean yellow mosaic virus. Virology 28:390-402, 1966.

Brasil

Brasil

Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e mosaico amarelo do feijoeiro

Electron microscopy of common and yellow bean mosaic viruses in infected tissues

Resumos

Datas de Publicação

Histórico