Panencefalite subaguda esclerosante: transmissão de agente encefalitogênico humano ao macaco rhesus

Alencar, Alexandre; Monteiro, Estácio

doi:10.1590/S0004-282X1971000400001

Resumos

Material proveniente do cérebro de um paciente com "panencefalite subaguda esclerosante" foi inoculado, por via intracerebral, em dois Macacus rhesus. Os animais permaneceram assintomáticos, aparentemente bem, por mais de um ano. Entretanto, 21 e 22 meses após as inoculações começaram a apresentar sinais de comprometimento neurológico, traduzido por paralisia dos membros posteriores, com apatia e caquexia progressivas. Os animais foram sacrificados. O exame histopatológico do sistema nervoso central mostrou gliose marginal e gliose da substância branca, com os neurônios exibindo sinais de "lesão celular crônica". Foram vistos discretos manguitos inflamatórios perivasculares. Em um animal foi encontrada inclusão acidófila intra- nuclear. Havia também proliferação da glia satélite perineuronal, com satelitose, notadamente no tronco cerebral. No exame das vísceras foi encontrado discreto processo de miocardite crônica. Foram retirados fragmentos dos encéfalos destes animais e inoculados em 4 outros, também por via intracerebral. Estes animais de 2.ª passagem após 2 meses de inoculação, em média, apresentaram sinais de comprometimento do sistema nervoso central semelhante ao dos animais doadores, de 1ª passagem. Houve, portanto, um encurtamento do período de inoculação da moléstia. Estes animais também foram sacrificados, sendo encontradas gliose marginal, gliose da substância branca, "lesão celular crônica" neuronal e proliferação da glia satélite, notadamente no tronco cerebral; vasos sangüíneos congestos; espongiose cortical. Os animais testemunhas, do mesmo lote, permaneceram normais. Acreditamos que as lesões observadas nos animais de 1.ª passagem, e que se repetiram com maior intensidade e com menor tempo de incubação nos de 2.ª passagem somente podem ser explicadas admitindo-se a existência de um agente virai que, pela natureza do quadro histopatológico (gliose, "lesão celular crônica" neuronal e espongiose cortical) bem como pelo período longo de incubação, deva pertencer ao grupo dos chamados vírus lentos.

Suspension of brain fragments obtained from a patient with subacute sclerosing panencephalitis (SSPE) were inoculated intracerebrally in two rhesus monkeys. The animals remained apparently well for more than one year after inoculation. Signs of neurological involvement (paralysis of the posterior limbs with apathy and cachexia) appeared respectively 21 and 22 months after inoculation. The animals were sacrified. At histological examination of the central nervous system marginal and white matter gliosis, and inflammatory perivascular cuffings were found; there was also proliferation of the perineural satelite glia with satelitosis in the brain stem. A discrete process of chronic myocarditis was observed on visceral examination. In one of these animals an acidophilic intranuclear inclusion was found. From these animals brain fragments were removed aseptically and inoculated intracerebrally in 4 others rhesus monkeys which, 2 to 3 months after inoculation, showed signs of central nervous system involvement similar to those observed in the first passage animals with a shorter incubation period. They were also sacrified. The histological examination showed lesions, similar to those found in the animals of the first passage, increased with cortical spongiosis. The lesions observed in the animals from the first passage and which were also present with more intensity and shorter incubation period in the second group of animals inoculated, seem to be produced by the so-called "slow viruses".

Panencefalite subaguda esclerosante: transmissão de agente encefalitogênico humano ao macaco rhesus

Subacute sclerosing panencephalitis: transmission of a human encephalitogenic agent to Macacus rhezus

Alexandre Alencar^I; Estácio Monteiro^II

^IChefe da Seção de Anatomia Patológica do Instituto Oswaldo Cruz, Chefe da Divisão de Neuropatologia do Instituto de Neurologia da UFRJ

^IIChefe da Seção de Virologia do Instituto Oswaldo Cruz (Rio de Janeiro, GB)

RESUMO

Material proveniente do cérebro de um paciente com "panencefalite subaguda esclerosante" foi inoculado, por via intracerebral, em dois Macacus rhesus. Os animais permaneceram assintomáticos, aparentemente bem, por mais de um ano. Entretanto, 21 e 22 meses após as inoculações começaram a apresentar sinais de comprometimento neurológico, traduzido por paralisia dos membros posteriores, com apatia e caquexia progressivas. Os animais foram sacrificados. O exame histopatológico do sistema nervoso central mostrou gliose marginal e gliose da substância branca, com os neurônios exibindo sinais de "lesão celular crônica". Foram vistos discretos manguitos inflamatórios perivasculares. Em um animal foi encontrada inclusão acidófila intra- nuclear. Havia também proliferação da glia satélite perineuronal, com satelitose, notadamente no tronco cerebral. No exame das vísceras foi encontrado discreto processo de miocardite crônica. Foram retirados fragmentos dos encéfalos destes animais e inoculados em 4 outros, também por via intracerebral. Estes animais de 2.ª passagem após 2 meses de inoculação, em média, apresentaram sinais de comprometimento do sistema nervoso central semelhante ao dos animais doadores, de 1ª passagem. Houve, portanto, um encurtamento do período de inoculação da moléstia. Estes animais também foram sacrificados, sendo encontradas gliose marginal, gliose da substância branca, "lesão celular crônica" neuronal e proliferação da glia satélite, notadamente no tronco cerebral; vasos sangüíneos congestos; espongiose cortical. Os animais testemunhas, do mesmo lote, permaneceram normais.

Acreditamos que as lesões observadas nos animais de 1.ª passagem, e que se repetiram com maior intensidade e com menor tempo de incubação nos de 2.ª passagem somente podem ser explicadas admitindo-se a existência de um agente virai que, pela natureza do quadro histopatológico (gliose, "lesão celular crônica" neuronal e espongiose cortical) bem como pelo período longo de incubação, deva pertencer ao grupo dos chamados vírus lentos.

SUMMARY

Suspension of brain fragments obtained from a patient with subacute sclerosing panencephalitis (SSPE) were inoculated intracerebrally in two rhesus monkeys. The animals remained apparently well for more than one year after inoculation. Signs of neurological involvement (paralysis of the posterior limbs with apathy and cachexia) appeared respectively 21 and 22 months after inoculation. The animals were sacrified. At histological examination of the central nervous system marginal and white matter gliosis, and inflammatory perivascular cuffings were found; there was also proliferation of the perineural satelite glia with satelitosis in the brain stem. A discrete process of chronic myocarditis was observed on visceral examination. In one of these animals an acidophilic intranuclear inclusion was found. From these animals brain fragments were removed aseptically and inoculated intracerebrally in 4 others rhesus monkeys which, 2 to 3 months after inoculation, showed signs of central nervous system involvement similar to those observed in the first passage animals with a shorter incubation period. They were also sacrified. The histological examination showed lesions, similar to those found in the animals of the first passage, increased with cortical spongiosis. The lesions observed in the animals from the first passage and which were also present with more intensity and shorter incubation period in the second group of animals inoculated, seem to be produced by the so-called "slow viruses".

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Instituto Oswaldo Cruz Manguinhos Rio de Janeiro, GB Brasil.

1. ALENCAR, A.; BROCK, M. & CAVALCANTI, J. L. S. Encefalites atípicas: estudo anátomo-clínico de 4 casos. An. 1.° Congresso Nacional de Medicina, Rio de Janeiro, julho de 1962.
2. ALENCAR, A.; BROCK, M. & CAVALCANTI, J. L. S. Encefalites atípicas: estudo anátomo-clínico de 6 casos. J. Bras. Neurol. 17:1, 1965.
3. ALENCAR, A. Alterações nucleares e nucleolares nas panencefalites sub-agudas esclerosantes. Arq. Neuro-Psiquiat. (São Paulo) 27:159, 1969.
4. ALENCAR, A.; OLIVEIRA, C. & COUTO, D. Considerações sobre as leucoencefalites. II Congresso Brasileiro de Neurologia, Rio de Janeiro, 1966.
5. ALPERS, M. P.; ADELS, B. R.; FUGE, C. & MEYER, J. Bibliography of subacute progressive panencephalitis (Dawson, Pette-Döring, Van Bogaert). Delayed measles panencephalitis. National Institute of Neurological Diseases and Blindness, Division of Research Services. National Institute of Health, Bethesda Md., August 1967, Revised July, 1, 1968.
6. BECKE, E. & DANIEL, P. M. Subacute degenerations of the brain transmissible to experimental animals: a neuropathological evaluation. An. VI Congr. Intern. Neuropathologie (Paris) : 858, 1970.
7. BOGAERT, L. VAN Une leucoencéphalite sclérosante subaigue. J. Neurol. Neurosurg. Psychiat. 8:101, 1945.
8. BOGAERT, L. VAN Sur une leucoencéphalite sclérosante subaigue (deuxième memoire). Rev. neurol. (Paris) 87:1, 1952.
9. BOGAERT, L. VAN Encephalitides. In Encephalitides: L. Van Bogaert, J. Hozay & A. Löwenthal, ed. Elsevier Publishing Co., Amsterdam, 1961.
10. BOUTEILLE, M.; FONTAINE, C.; VEDRENNE, C. & DELARUE, J. Sur un cas d'encéphalite subaigue à inclusions; étude anatomo-clinique. Rev. neurol. (Paris) 113:454, 1965.
11. BRODANO, G. B.; OYANAGI, S.; KATZ, M. & al. Presence of two different viral agents in brain cells of patients with subacute sclerosing panencephalitis. Proc. Soc. Exp. Biol. Med. 134:230, 1970.
12. BYINGTON, D. P.; CASTRO, A. E. & BURNSTEIN, T. Adaptation to hamsters of a neurotropic measles virus from subacute sclerosing panencephalitis. Nature (London) 255:554, 1970.
13. DAWSON, Jr., J. R. Cellular inclusions in cerebral lesions of lethargic encephalitis. Am. J. Path. 9:7, 1933.
14. DAWSON, Jr., J. R. Cellular inclusions in cerebral lesions of epidemic encephalitis: second report. Arch. Neurol. Psichiat. (Chicago) 31:685, 1934.
15. DAYAN, A. D.; GORTLING, J. V. T.; GREAVES, J. L.; STEVENS, D. W. & WOODHOUSE, M. A. Evidence of a pseudomyxovirus in the brain in subacute sclerosing leucoencephalitis. Lancet 1:7497, 1967.
16. EAGLE, H. Amino acid metabolism in mammalian cell cultures. Science 130:432, 1959.
17. ECONOMO, C. Von Encephalitis Lethargica: its Sequellae and Treatment. Oxford University Press, London, 1931.
18. Editorial Measles virus and subacute sclerosing panencephalitis. Neurology (Minneapolis) 18:2, 1968.
19. FRAZER, H. - Comparative morphology of ageing and scrapie. An. VI. Congr. Intern. Neuropathologie (Paris) : 897, 1970.
20. GAJDUSEK, D. C.; GIBBS, C. J. Jr. & ALPERS, M. Experimental transmission of a "kuru-like" syndrome to chimpanzees. Nature (London) 207:794, 1966.
21. GAJDUSEK, D. C.; GIBBS, C. J. Jr. & ALPERS, M. Transmission and passage of "kuru" in chimpanzees. Science 155:212, 1967.
22. GIBBS Jr., C. J. & GAJDUSEK, D. C. Infection as the etiology of spongiform encephalopathy (Creutzfeldt Jacob disease). Science (London) 165:1023, 1969.
23. GIBBS Jr., C. J.; GAJDUSEK, D. C; ASHER, D. M.; ALPERS, M. P.; BECK, E.; DANIEL, P. M. & METTEWS, W. B. Creutzfeldt Jacob disease (spongiforme encephalopathy): transmission to the chimpanzee. Science (London) 161:388, 1968.
24. GONATAS, N. K.; TERRY, R. D. & WEIS, M. Electron microscopic studies in two cases of Jacob-Creutzfeldt disease. J. Neuropath, exp. Neurol. 27:575, 1965.
25. HORTA-BARBOSA, L.; FUCCILLO, D. A.; SEVER, J. L. & ZEMAN, W. Subacute sclerosing panencephalitis: isolation of measles virus from brain biopsy. Nature 221:974, 1969.
26. INNES, J. M. R. & SAUNDERS, L. Z. S. Confluent leucoencephalosis and perivascular myelosis. In Comparative Neuropathology. Academic Press, New York-London, 1962.
27. KATZ, M.; RORKE, L. B.; MASLAND, W. S.; KOPROWSKI, H. & TUCKER, S. H. Transmission of encephalitogenic agent from brains of patients with subacute sclerosing panencephalitis to ferrets. New England J. Med. 279:793, 1968.
28. KATZ, M.; RORKE, L. B.; MASLAND, W. S.; BRODANO, G. B. & KOPROWSKI, H. Subacute sclerosing panencephalitis: isolation of a virus encephalitogenic for ferrets. Incet. Dis. 121:188, 1970.
29. LEHRICH, J. R.; KATZ, M.; RORKE, L. B.; BARBANTI-BRODANO, G. & KOPROWSKI, H. Subacute sclerosing panencephalitis: encephalitis in hamsters produced by viral agents isolated from human brain cells. Arch. Neurol. (Chicago) 23:97, 1970.
30. MARGOLIS, G. & KILHAM, L. In pursuit of an ataxic hamster or. virus-induced cerebellar hypoplasia. In The Central Nervous System. Some Experimental Models of Neurological Diseases: Orville T. Bailey & David, E. Smith, ed. The William & Wilkins Co., Baltimore, 1968.
31. MATUMOTO, M.; SABURI, Y.; AOYAMA, Y. & al. A neurotropic variant of measles virus in suckling mice. Arch ges. Virusforsch. 14:683, 1964.
32. MOORE, A. E.; SABACHEWSKY, L. & TOOLAN, H. W. Cultures characteristics of four permanent lines of human cancer cells. Cancer Res. 15:598, 1955.
33. NAGEL, I. T. Subacute sclerosing leucoencephalitis: ultrastructure of intranuclear and intracytoplasmic inclusions. Science 154:899, 1966.
34. PAYNE, F. E.; BAUBLIS, J. V. & ITABASHI, H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England J. Med. 281:585, 1969.
35. PELC, A.; PÉRIER, J. O. & QUERSIN-THIRY, L. Résultats expérimentaux obtenus dans l'encéphalite humaine, type encéphalite subaigue à inclusions, leucoencéphalite sclérosante subaigue. Rev. neurol. (Paris): 98:3, 1958.
36. PERIER, O. & VANDERHAEGHEN, J. J. Indications etiologiques apportées par la microscopie électronique dans certaines encéphalites humaines. Rev. neurol. (Paris) 115:250, 1965.
37. PETTE, H. & DORING, G. Ueber einheimische Panencephalomyelitis von Charakter der Encephalitis japonica. Dtsch. Arch. Nervenheilk. 149:7, 1939.
38. SHAW, C. M.; BUCHAN, G. C. & COLDEVIN, B. C. Myxovirus as a possible etiologic agent in subacute inclusion body encephalitis. New England J. Med. 277:511, 1967.
39. TER MEULEN, V.; MULLER, D.; KATZ, M. & al. Immunohistological, microscopical and neurochemical studies on encephalitidis: IV. Subacute sclerosing (progressive) panencephalitis. Histochemical and imunohistological findings in tissue cultures derived from SSPE brain biopsies. Acta Neuropath. 15:1, 1970.
40. WAKSMAN, B. H.; BURNSTEIN, T. & ADAMS, R. D. Histologie study of the encephalomyelitis produced in hamsters by a neurotropic strain of measles. J. Neuropath, exp. Neurol. 21:25, 1962.
41. YASUMURA, Y. & KAWAKITA, Y. Research into SV40 by tissue culture. Nippon Sinsho 21:1209, 1963.

Datas de Publicação

Publicação nesta coleção
19 Abr 2013
Data do Fascículo
Dez 1971

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALENCAR, A.; BROCK, M. & CAVALCANTI, J. L. S. Encefalites atípicas: estudo anátomo-clínico de 4 casos. An. 1.° Congresso Nacional de Medicina, Rio de Janeiro, julho de 1962.

[2] 2. ALENCAR, A.; BROCK, M. & CAVALCANTI, J. L. S. Encefalites atípicas: estudo anátomo-clínico de 6 casos. J. Bras. Neurol. 17:1, 1965.

[3] 3. ALENCAR, A. Alterações nucleares e nucleolares nas panencefalites sub-agudas esclerosantes. Arq. Neuro-Psiquiat. (São Paulo) 27:159, 1969.

[4] 4. ALENCAR, A.; OLIVEIRA, C. & COUTO, D. Considerações sobre as leucoencefalites. II Congresso Brasileiro de Neurologia, Rio de Janeiro, 1966.

[5] 5. ALPERS, M. P.; ADELS, B. R.; FUGE, C. & MEYER, J. Bibliography of subacute progressive panencephalitis (Dawson, Pette-Döring, Van Bogaert). Delayed measles panencephalitis. National Institute of Neurological Diseases and Blindness, Division of Research Services. National Institute of Health, Bethesda Md., August 1967, Revised July, 1, 1968.

[6] 6. BECKE, E. & DANIEL, P. M. Subacute degenerations of the brain transmissible to experimental animals: a neuropathological evaluation. An. VI Congr. Intern. Neuropathologie (Paris) : 858, 1970.

[7] 7. BOGAERT, L. VAN Une leucoencéphalite sclérosante subaigue. J. Neurol. Neurosurg. Psychiat. 8:101, 1945.

[8] 8. BOGAERT, L. VAN Sur une leucoencéphalite sclérosante subaigue (deuxième memoire). Rev. neurol. (Paris) 87:1, 1952.

[9] 9. BOGAERT, L. VAN Encephalitides. In Encephalitides: L. Van Bogaert, J. Hozay & A. Löwenthal, ed. Elsevier Publishing Co., Amsterdam, 1961.

[10] 10. BOUTEILLE, M.; FONTAINE, C.; VEDRENNE, C. & DELARUE, J. Sur un cas d'encéphalite subaigue à inclusions; étude anatomo-clinique. Rev. neurol. (Paris) 113:454, 1965.

[11] 11. BRODANO, G. B.; OYANAGI, S.; KATZ, M. & al. Presence of two different viral agents in brain cells of patients with subacute sclerosing panencephalitis. Proc. Soc. Exp. Biol. Med. 134:230, 1970.

[12] 12. BYINGTON, D. P.; CASTRO, A. E. & BURNSTEIN, T. Adaptation to hamsters of a neurotropic measles virus from subacute sclerosing panencephalitis. Nature (London) 255:554, 1970.

[13] 13. DAWSON, Jr., J. R. Cellular inclusions in cerebral lesions of lethargic encephalitis. Am. J. Path. 9:7, 1933.

[14] 14. DAWSON, Jr., J. R. Cellular inclusions in cerebral lesions of epidemic encephalitis: second report. Arch. Neurol. Psichiat. (Chicago) 31:685, 1934.

[15] 15. DAYAN, A. D.; GORTLING, J. V. T.; GREAVES, J. L.; STEVENS, D. W. & WOODHOUSE, M. A. Evidence of a pseudomyxovirus in the brain in subacute sclerosing leucoencephalitis. Lancet 1:7497, 1967.

[16] 16. EAGLE, H. Amino acid metabolism in mammalian cell cultures. Science 130:432, 1959.

[17] 17. ECONOMO, C. Von Encephalitis Lethargica: its Sequellae and Treatment. Oxford University Press, London, 1931.

[18] 18. Editorial Measles virus and subacute sclerosing panencephalitis. Neurology (Minneapolis) 18:2, 1968.

[19] 19. FRAZER, H. - Comparative morphology of ageing and scrapie. An. VI. Congr. Intern. Neuropathologie (Paris) : 897, 1970.

[20] 20. GAJDUSEK, D. C.; GIBBS, C. J. Jr. & ALPERS, M. Experimental transmission of a "kuru-like" syndrome to chimpanzees. Nature (London) 207:794, 1966.

[21] 21. GAJDUSEK, D. C.; GIBBS, C. J. Jr. & ALPERS, M. Transmission and passage of "kuru" in chimpanzees. Science 155:212, 1967.

[22] 22. GIBBS Jr., C. J. & GAJDUSEK, D. C. Infection as the etiology of spongiform encephalopathy (Creutzfeldt Jacob disease). Science (London) 165:1023, 1969.

[23] 23. GIBBS Jr., C. J.; GAJDUSEK, D. C; ASHER, D. M.; ALPERS, M. P.; BECK, E.; DANIEL, P. M. & METTEWS, W. B. Creutzfeldt Jacob disease (spongiforme encephalopathy): transmission to the chimpanzee. Science (London) 161:388, 1968.

[24] 24. GONATAS, N. K.; TERRY, R. D. & WEIS, M. Electron microscopic studies in two cases of Jacob-Creutzfeldt disease. J. Neuropath, exp. Neurol. 27:575, 1965.

[25] 25. HORTA-BARBOSA, L.; FUCCILLO, D. A.; SEVER, J. L. & ZEMAN, W. Subacute sclerosing panencephalitis: isolation of measles virus from brain biopsy. Nature 221:974, 1969.

[26] 26. INNES, J. M. R. & SAUNDERS, L. Z. S. Confluent leucoencephalosis and perivascular myelosis. In Comparative Neuropathology. Academic Press, New York-London, 1962.

[27] 27. KATZ, M.; RORKE, L. B.; MASLAND, W. S.; KOPROWSKI, H. & TUCKER, S. H. Transmission of encephalitogenic agent from brains of patients with subacute sclerosing panencephalitis to ferrets. New England J. Med. 279:793, 1968.

[28] 28. KATZ, M.; RORKE, L. B.; MASLAND, W. S.; BRODANO, G. B. & KOPROWSKI, H. Subacute sclerosing panencephalitis: isolation of a virus encephalitogenic for ferrets. Incet. Dis. 121:188, 1970.

[29] 29. LEHRICH, J. R.; KATZ, M.; RORKE, L. B.; BARBANTI-BRODANO, G. & KOPROWSKI, H. Subacute sclerosing panencephalitis: encephalitis in hamsters produced by viral agents isolated from human brain cells. Arch. Neurol. (Chicago) 23:97, 1970.

[30] 30. MARGOLIS, G. & KILHAM, L. In pursuit of an ataxic hamster or. virus-induced cerebellar hypoplasia. In The Central Nervous System. Some Experimental Models of Neurological Diseases: Orville T. Bailey & David, E. Smith, ed. The William & Wilkins Co., Baltimore, 1968.

[31] 31. MATUMOTO, M.; SABURI, Y.; AOYAMA, Y. & al. A neurotropic variant of measles virus in suckling mice. Arch ges. Virusforsch. 14:683, 1964.

[32] 32. MOORE, A. E.; SABACHEWSKY, L. & TOOLAN, H. W. Cultures characteristics of four permanent lines of human cancer cells. Cancer Res. 15:598, 1955.

[33] 33. NAGEL, I. T. Subacute sclerosing leucoencephalitis: ultrastructure of intranuclear and intracytoplasmic inclusions. Science 154:899, 1966.

[34] 34. PAYNE, F. E.; BAUBLIS, J. V. & ITABASHI, H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England J. Med. 281:585, 1969.

[35] 35. PELC, A.; PÉRIER, J. O. & QUERSIN-THIRY, L. Résultats expérimentaux obtenus dans l'encéphalite humaine, type encéphalite subaigue à inclusions, leucoencéphalite sclérosante subaigue. Rev. neurol. (Paris): 98:3, 1958.

[36] 36. PERIER, O. & VANDERHAEGHEN, J. J. Indications etiologiques apportées par la microscopie électronique dans certaines encéphalites humaines. Rev. neurol. (Paris) 115:250, 1965.

[37] 37. PETTE, H. & DORING, G. Ueber einheimische Panencephalomyelitis von Charakter der Encephalitis japonica. Dtsch. Arch. Nervenheilk. 149:7, 1939.

[38] 38. SHAW, C. M.; BUCHAN, G. C. & COLDEVIN, B. C. Myxovirus as a possible etiologic agent in subacute inclusion body encephalitis. New England J. Med. 277:511, 1967.

[39] 39. TER MEULEN, V.; MULLER, D.; KATZ, M. & al. Immunohistological, microscopical and neurochemical studies on encephalitidis: IV. Subacute sclerosing (progressive) panencephalitis. Histochemical and imunohistological findings in tissue cultures derived from SSPE brain biopsies. Acta Neuropath. 15:1, 1970.

[40] 40. WAKSMAN, B. H.; BURNSTEIN, T. & ADAMS, R. D. Histologie study of the encephalomyelitis produced in hamsters by a neurotropic strain of measles. J. Neuropath, exp. Neurol. 21:25, 1962.

[41] 41. YASUMURA, Y. & KAWAKITA, Y. Research into SV40 by tissue culture. Nippon Sinsho 21:1209, 1963.

Brasil

Brasil

Panencefalite subaguda esclerosante: transmissão de agente encefalitogênico humano ao macaco rhesus

Subacute sclerosing panencephalitis: transmission of a human encephalitogenic agent to Macacus rhezus

Resumos

Datas de Publicação