Experimental paracoccidioidomycosis in hamster: transmission electron microscopy of inoculation site lesion

Coelho, K. I. R.; Takeo, K.; Yamaguchi, M.; Sano, A.; Kurita, N.; Yoshida, S.; Nishimura, K.; Miyaji, M.

doi:10.1590/S0036-46651994000300005

Abstracts

Interaction between Paracoccidioides brasiliensis (Pb) and inflammatory cells in hamster testis was studied sequentially by transmission electron microscopy. In early lesions (six hours after inoculation), polymorphonuclear neutrophils (PMNs) were the major and mononuclear cells and eosinophils were the minor constituents of the inflammatory cells. PMNs were later replaced by mononuclear cells. Viable Pb cells were phagocytosed or surrounded by inflammatory cells. Preserved Pb cells usually had broad host-parasite interphases, whereas dying ones had narrow interphases. The outer layer of the fungus wall was sometimes broken by PMN in some focal points, broken pieces being peeled off and phagocytosed. Small Pb cells were uninuclear, and were often related to broad interphase. Large Pb cells were multinucleated with irregularly shaped wall, and sometimes had lomasome and/or myelin like structures. Different interaction patterns of Pb with inflammatory cells may be due to functionally different host cell flow to the inoculation site or due to the age of Pb cells or both.

Paracoccidioidomycosis; Intratesticular Inoculation; Transmission Electron Microscopy

Estudou-se sequencialmente, à microscopia eletrônica de transmissão, a interação entre Paracoccidioides brasiliensis (Pb) e células inflamatórias em hamsters inoculados por via intratesticular. Seis horas após inoculações havia predominância de neutrófilos, estando presentes algumas células mononucleares e eosinófilos. Os neutrófilos foram progressivamente substituídos por células mononucleares. Fungos viáveis apresentavam-se fagocitados ou circunscritos por células inflamatórias, geralmente com ampla interface hospedeiro-parasita. Fungos mortos ou degenerados eram acompanhados de interfase estreita. A camada externa da parede do Pb era às vezes quebrada quando em contacto com neutrófilos, em vários pontos, sendo os fragmentos dessa parede descamados e fagocitados. Células fúngicas pequenas com um único núcleo se relacionavam com ampla interfase enquanto as células maiores e multinucleadas apresentavam paredes irregulares, por vezes, contendo lomasoma e/ou estrutura semelhante à mielina. Diferentes padrões de interação do Pb com células do hospedeiro podem ser decorrentes do a fluxo de células inflamatórias funcionalmente diferentes ao local de inoculação ou à idade dos fungos ou ambos os fatores.

MYCOLOGY

Experimental paracoccidioidomycosis in hamster: transmission electron microscopy of inoculation site lesion

Paracoccidioidomicose experimental em hamster: microscopía eletrônica de transmissão da lesão do local de inoculação

K. I. R. Coelho^I; K. Takeo; M. Yamaguchi^II; A. Sano; N. Kurita; S. Yoshida; K. Nishimura; M. Miyaji

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba-Shi, Chiba 260, JAPAN

^IDepartment of Pathology, Faculty of Medicine, UNESP, 18618-000, Botucatu, São Paulo, Brazil

^IIInstitute of Basic Medicine, Jikei Medical University, 3-25-8 Nishi-Shinbashi, Minato-Ku, Tokyo, 105 JAPAN

^{Address for correspondence} Address for correspondence: K. I. R. COELHO Department of Pathology, Faculty of Medicine, UNESP 18618-000 Botucatu, São Paulo, Brazil Fax (0149)-21-23-48

SUMMARY

Interaction between Paracoccidioides brasiliensis (Pb) and inflammatory cells in hamster testis was studied sequentially by transmission electron microscopy. In early lesions (six hours after inoculation), polymorphonuclear neutrophils (PMNs) were the major and mononuclear cells and eosinophils were the minor constituents of the inflammatory cells. PMNs were later replaced by mononuclear cells. Viable Pb cells were phagocytosed or surrounded by inflammatory cells. Preserved Pb cells usually had broad host-parasite interphases, whereas dying ones had narrow interphases. The outer layer of the fungus wall was sometimes broken by PMN in some focal points, broken pieces being peeled off and phagocytosed. Small Pb cells were uninuclear, and were often related to broad interphase. Large Pb cells were multinucleated with irregularly shaped wall, and sometimes had lomasome and/or myelin like structures. Different interaction patterns of Pb with inflammatory cells may be due to functionally different host cell flow to the inoculation site or due to the age of Pb cells or both.

Keywords: Paracoccidioidomycosis; Intratesticular Inoculation; Transmission Electron Microscopy.

RESUMO

Estudou-se sequencialmente, à microscopia eletrônica de transmissão, a interação entre Paracoccidioides brasiliensis (Pb) e células inflamatórias em hamsters inoculados por via intratesticular. Seis horas após inoculações havia predominância de neutrófilos, estando presentes algumas células mononucleares e eosinófilos. Os neutrófilos foram progressivamente substituídos por células mononucleares. Fungos viáveis apresentavam-se fagocitados ou circunscritos por células inflamatórias, geralmente com ampla interface hospedeiro-parasita. Fungos mortos ou degenerados eram acompanhados de interfase estreita. A camada externa da parede do Pb era às vezes quebrada quando em contacto com neutrófilos, em vários pontos, sendo os fragmentos dessa parede descamados e fagocitados. Células fúngicas pequenas com um único núcleo se relacionavam com ampla interfase enquanto as células maiores e multinucleadas apresentavam paredes irregulares, por vezes, contendo lomasoma e/ou estrutura semelhante à mielina. Diferentes padrões de interação do Pb com células do hospedeiro podem ser decorrentes do a fluxo de células inflamatórias funcionalmente diferentes ao local de inoculação ou à idade dos fungos ou ambos os fatores.

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ACKNOWLEDGEMENT

During the course of these studies Dra. K. I. R. Coelho was supported by the Japanese Ministry of Education, Science and Culture as a guest professor-foreign researcher at Chiba University Research Center for Pathogenic Fungi and Microbial Toxicoses.

Recebido para publicação em 11/08/1993.

Aceito para publicação em 27/09/1993.

1. ALVES, L.M.C.; FIGUEIREDO, F.; BRANDĂO FILHO, S.L.; TINCANI, I. & SILVA, C.L. - The role of fractions from Paracoccidioides brasiliensis in the genesis of inflammatory response. Mycopathologia (Den Haag), 97: 3-7, 1987.
2. CALICH, V.L.G. & BURGER, E. - PMN chemotactic factor produced by glass-adherent cells in the acute inflammation by Paracoccidioides brasiliensis. Brit. J. exp. Path., 66: 57-65, 1985.
3. CANO, L.E.; ARANGO, R.; SALAZAR, M.E. et al. - Killing of Paracoccidioides brasiliensis by pulmonary macrophages and the effect of cytokines. J. med. vet. Mycol., 30: 161-168, 1992.
4. CARBONELL, L.M. - Cell wall changes during the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis. J. Bact., 94: 213-223, 1967.
5. CARBONELL, L.M. & GIL, F. - Ultraestrutura del Paracoccidioides brasiliensis. In: DEL NEGRO, G; LACAZ, C. da S. & FIORILLO, A.M., ed. - Paracoccidioidomicose. Blastomicose sul americana. Săo Paulo, SARVIER-EDUSP, 1982. p. 23-24.
6. FAVA NETTO, C.; BRITO, T. & LACAZ, C. da S. - Experimental South American blastomycosis of the guinea-pig. An immunologie and pathologic study. Path. et Microbiol. (Basel), 24: 192-206, 1961.
7. FURTADO, J.S.; BRITO, T. & FREYMULLER, E. - The structure and reproduction of Paracoccidioides brasiliensis in human tissue. Sabouraudia, 23: 245-251, 1967.
8. GOIHMAN-YAHR, M.; ESSENFELD-YAHR, E.; ALBORNOZ, M.C. et al. - New method for estimating digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro. J. clin. Microbiol., 10: 365-370, 1979.
9. GOIHMAN-YAHR, M.; ESSENFELD-YAHR, E.; ALBORNOZ, M.C. et al. - Defect of in vitro digestive ability of polymorphonuclear leukocytes in paracoccidioidomycosis. Infect. Immun., 28: 557-566, 1980.
10. GOIHMAN-YAHR, M.; ROTHENBERG, A.; ROSQUETE, R. et al. - A novel method for estimating killing ability and digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro. Sabouraudia, 23: 245-251, 1985.
11. IABUKI, K. & MONTENEGRO, M.R. - Experimental paracoccidioidomycosis in Syrian hamster: morphology, ultra-structure and correlation of lesion with presence of specific antigen and serum levels of antibodies. Mycopathologia (Den Haag), 67: 131-141, 1979.
12. LARRONDO, J.V. & CALVO, M.A. - Ultrastructure of concentric membrane system in Arthrinium aureum. Mycopathologia (Den Haag), 117: 153-155, 1992.
13. McEWEN, J.G.; BRUMMER, E.; STEVENS, D.A. & RESTREPO, A. - Effect of murine polymorphonuclear leukocytes on the yeast form of Paracoccidioides brasiliensis. Amer. J. trop. Med. Hyg., 36: 603-608, 1987.
14. MINGUETTI, G.; HOFMEISTER, R.M.; FAVARO, M. & FREITAS, O.T. - Ultraestrutura do Paracoccidioides brasiliensis - na fase leveduriforme. Rev. Inst. Med. Crop. S. Paulo, 25: 161-167, 1983.
15. SAN-BLAS, G. - The cell wall of fungal human pathogens: its possible role in host-parasite relationship. Mycopathologia (Den Haag), 79: 159-184, 1982.
16. SESSO, A. & BARUZZI, R.G. - Interaction between macrophage and parasite cells in lobomycosis. The thickened cell wall of Paracoccidioides loboi exhibits apertures to the extracellular milieu. J. Submicrosc. Cytol. Path., 20: 537-548, 1988.
17. URIBE, F.; ZULUAGA, A.I.; LEON, W. & RESTREPO, A. - Histopathology of cutaneous and mucosa lesions in human paracoccidioidomycosis. Rev. Inst. Med. trop. S. Paulo, 29: 90-96, 1987.

Address for correspondence:

K. I. R. COELHO

Department of Pathology, Faculty of Medicine, UNESP

18618-000 Botucatu, São Paulo, Brazil

Fax (0149)-21-23-48

Publication Dates

Publication in this collection
20 Sept 2006
Date of issue
June 1994

History

Received
11 Aug 1993
Accepted
27 Sept 1993

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALVES, L.M.C.; FIGUEIREDO, F.; BRANDĂO FILHO, S.L.; TINCANI, I. & SILVA, C.L. - The role of fractions from Paracoccidioides brasiliensis in the genesis of inflammatory response. Mycopathologia (Den Haag), 97: 3-7, 1987.

[2] 2. CALICH, V.L.G. & BURGER, E. - PMN chemotactic factor produced by glass-adherent cells in the acute inflammation by Paracoccidioides brasiliensis. Brit. J. exp. Path., 66: 57-65, 1985.

[3] 3. CANO, L.E.; ARANGO, R.; SALAZAR, M.E. et al. - Killing of Paracoccidioides brasiliensis by pulmonary macrophages and the effect of cytokines. J. med. vet. Mycol., 30: 161-168, 1992.

[4] 4. CARBONELL, L.M. - Cell wall changes during the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis. J. Bact., 94: 213-223, 1967.

[5] 5. CARBONELL, L.M. & GIL, F. - Ultraestrutura del Paracoccidioides brasiliensis. In: DEL NEGRO, G; LACAZ, C. da S. & FIORILLO, A.M., ed. - Paracoccidioidomicose. Blastomicose sul americana. Săo Paulo, SARVIER-EDUSP, 1982. p. 23-24.

[6] 6. FAVA NETTO, C.; BRITO, T. & LACAZ, C. da S. - Experimental South American blastomycosis of the guinea-pig. An immunologie and pathologic study. Path. et Microbiol. (Basel), 24: 192-206, 1961.

[7] 7. FURTADO, J.S.; BRITO, T. & FREYMULLER, E. - The structure and reproduction of Paracoccidioides brasiliensis in human tissue. Sabouraudia, 23: 245-251, 1967.

[8] 8. GOIHMAN-YAHR, M.; ESSENFELD-YAHR, E.; ALBORNOZ, M.C. et al. - New method for estimating digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro. J. clin. Microbiol., 10: 365-370, 1979.

[9] 9. GOIHMAN-YAHR, M.; ESSENFELD-YAHR, E.; ALBORNOZ, M.C. et al. - Defect of in vitro digestive ability of polymorphonuclear leukocytes in paracoccidioidomycosis. Infect. Immun., 28: 557-566, 1980.

[10] 10. GOIHMAN-YAHR, M.; ROTHENBERG, A.; ROSQUETE, R. et al. - A novel method for estimating killing ability and digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro. Sabouraudia, 23: 245-251, 1985.

[11] 11. IABUKI, K. & MONTENEGRO, M.R. - Experimental paracoccidioidomycosis in Syrian hamster: morphology, ultra-structure and correlation of lesion with presence of specific antigen and serum levels of antibodies. Mycopathologia (Den Haag), 67: 131-141, 1979.

[12] 12. LARRONDO, J.V. & CALVO, M.A. - Ultrastructure of concentric membrane system in Arthrinium aureum. Mycopathologia (Den Haag), 117: 153-155, 1992.

[13] 13. McEWEN, J.G.; BRUMMER, E.; STEVENS, D.A. & RESTREPO, A. - Effect of murine polymorphonuclear leukocytes on the yeast form of Paracoccidioides brasiliensis. Amer. J. trop. Med. Hyg., 36: 603-608, 1987.

[14] 14. MINGUETTI, G.; HOFMEISTER, R.M.; FAVARO, M. & FREITAS, O.T. - Ultraestrutura do Paracoccidioides brasiliensis - na fase leveduriforme. Rev. Inst. Med. Crop. S. Paulo, 25: 161-167, 1983.

[15] 15. SAN-BLAS, G. - The cell wall of fungal human pathogens: its possible role in host-parasite relationship. Mycopathologia (Den Haag), 79: 159-184, 1982.

[16] 16. SESSO, A. & BARUZZI, R.G. - Interaction between macrophage and parasite cells in lobomycosis. The thickened cell wall of Paracoccidioides loboi exhibits apertures to the extracellular milieu. J. Submicrosc. Cytol. Path., 20: 537-548, 1988.

[17] 17. URIBE, F.; ZULUAGA, A.I.; LEON, W. & RESTREPO, A. - Histopathology of cutaneous and mucosa lesions in human paracoccidioidomycosis. Rev. Inst. Med. trop. S. Paulo, 29: 90-96, 1987.

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