Diphyllobothrium latum infection in a non-endemic country: case report

Infecção pelo Diphyllobothrium latum em país não endêmico: relato de caso

RELATO DE CASO CASE REPORT

Diphyllobothrium latum infection in a non-endemic country: case report

Infecção pelo Diphyllobothrium latum em país não endêmico: relato de caso

Mauricio M. Llaguno^I; Juan Cortez-Escalante^I; Jitra Waikagul^II; Ana Carolina Guimarães Faleiros^III; Francisco das Chagas^I; Cleudson Castro^I

^INúcleo de Medicina Tropical, Universidade de Brasília, Brasília, DF

^IIMahidol University, Bangkok, Thaliand

^IIICurso de Pós-Graduação em Medicina Tropical, Universidade Federal do Triângulo Mineiro, Uberaba, MG

^{Address to}

ABSTRACT

Diphyllobothriasis, which is rarely described in Brazil, was reported initially as a travelers' disease and as an accidental infection in individuals who ate raw freshwater fish. This report aims to present the case of a 20-year-old patient with confirmed Diphyllobothrium latum infection.

Key-words: Diphyllobothriasis. Diphyllobothrium latum. Emerging disease.

RESUMO

A difilobotríase, raramente descrita no Brasil, foi referida inicialmente como doença de viajantes e como infecção acidental em indivíduos que se alimentam com peixe cru de água doce. Este relato objetiva apresentar o caso de uma paciente de 20 anos com infecção confirmada pelo Diphyllobothrium latum.

Palavras-chaves: Difilobotríase. Diphyllobothrium latum. Doença emergente.

Diphyllobothriasis is an ichthyic zoonosis accidentally acquired by humans^{7 11}, with worldwide distribution and sporadic outbreaks. Cases in South America are restricted to Chile, Peru, Argentina and Brazil^{6 10}

Humans become infected by ingesting larvae (plerocercoids) in raw or poorly cooked freshwater fish. Several species of diphyllobothriids have been reported in humans: Diphyllobothrium latum, Diphyllobothrium pacificum and Diphyllobothrium nihonkaiense have been commonly associated with human cases, generally infecting the small intestine^{9 12}.

Most infections are asymptomatic. However, some cases have been associated with megaloblastic anemia, gastrointestinal discomfort, anorexia, nausea, vomiting, asthenia, weight loss, eosinophilia and diarrhea. Occasionally, infection may cause acute abdominal pain and intestinal obstruction. In rare circumstances, migration of proglottids can cause cholangitis or cholecystitis⁸. The associated symptomatology is non-specific, but megaloblastic anemia is a well-described common complication in Scandinavian patients.

The first cases of diphyllobothriasis in Brazil were observed by Pirajá da Silva in Bahia in 1915, in a Scandinavian sailor, and by Samuel Pessoa in São Paulo, in a French woman who lived in Switzerland^{2 6}. After a long gap, several cases were diagnosed in 2004, in patients who had the habit of eating sushi and sashimi containing raw fish⁵. This paper presents an autochthonous case of Diphyllobothrium latum infection in Brasília, Federal District, Brazil.

CASE REPORT

A 20-year-old woman living in Brasília presented to the outpatient service of the University Hospital of Brasilia (HUB), in 2006, after intestinal expulsion of a large white-ribbon parasite and the use of large amounts of mebendazole (200mg bid for 4 days) and praziquantel (600mg tid for 3 days). At her consultation with the physician, she reported that she had been suffering from gastrointestinal disorders such as abdominal cramps, flatulence, sporadic diarrhea, polyphagia and other unspecific symptoms like intense general itching and irritability for the preceding two years. She revealed that she often ate smoked meat products like pork and bacon and that she ate raw fish in sushi and sashimi in three local Japanese restaurants, twice a week. She had a family history of cysticercosis and taeniasis without ever having received treatment.

Her laboratory test results did not show any remarkable abnormalities: RBC 4.7 x 10⁶/µ l, hemoglobin 13.7g/dl, hematocrit 42%, WBC 8.6 x 10³/µ l, platelets 316 x 10³/µ l, MCV 88.2 fl, MCH 28.8pg and eosinophil count 6%.

The morphology and histology of the parasite were investigated. The adult worm appeared to be segmented without a scolex and it was 38 cm in length, with a total count of 114 proglottids, each measuring approximately 0.80 mm in width by 0.29 mm in length (Figure 1A). The mature and gravid proglottids were broader than their length, with a typical rosette-shaped uterus (Figure 1B). A longitudinal histological section (Figure 1C) showed the cirrus sac (1), ovaries (2), vitellaria (3) and seminal sac (4). The eggs (Figure 1D) measured 64 ± 5µ m by 43 ± 3µ m, and had an operculum (a), a knob (b) and a moderately thick shell. From these characteristics, the parasite matched with Diphyllobothrium latum.

DISCUSSION

This was an autochthonous case of Diphyllobothrium latum. The previous outbreaks in Brazil occurred at the end of 2004 and in the first quarter of 2005. This suggests that diphyllobothriasis is expanding, but that the sources of the fish are not still clear. Twenty-three cases of diphyllobothriasis were reported as a result of laboratory detection of eggs or proglottids of Diphyllobothrium latum during this period⁵.

Finding an operculated egg with a small knob on the anti-operculum side is the most accurate diagnostic characteristic for identifying diphyllobothriids. However, the size of their eggs varies from one species of Diphyllobothrium to another³. According to some reports, the eggs of Diphyllobothrium pacificum are 40-60µ m in length and 36-40µ m in width¹, while the egg size of Diphyllobothrium latum is approximately 58-76 by 40-51µ m⁴.

Some authors have supported the hypothesis that fish may be the source of Diphyllobothrium latum plerocercoid infection. Another possibility is that the life cycle of Diphyllobothrium latum may have become established along the Brazilian coast and in the country's rivers⁹.

Epidemiological investigations are being conducted in São Paulo to identify the source of Diphyllobothrium latum plerocercoids. These may help towards implementing educational and sanitary measures aimed at preventing diphyllobothriasis from becoming endemic in Brazil⁵.

Diphyllobothriasis is becoming an emerging disease in Brazil, due probably to the expansion in the habit of eating raw or partially cooked fish.

Recebido para publicação em: 24/01/2008

Aceito em: 05/06/2008

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Publication Dates

• Publication in this collection
  14 Aug 2008
• Date of issue
  June 2008

History

• Accepted
  05 June 2008
• Received
  24 Jan 2008