Abstract
We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
concanavalin; mononuclear cells; visceral leishmaniasis
A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
Manoel Barral-Netto1
A. Barral2
FIOCRUZ, Centro de Pesquisas Gonçalo Muniz, Brasil
UFBa, Hospital Prof. Edgarf Santos, Laboratório de Imunologia, Salvador, Brasil
We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
ABSTRACT
Descrevemos um método simples para determinação da ligação da Concanavalina A na superfície de células mononucleares do sangue periférico humano. A ligação é revelada por método imuno-enzimático utilizando imunoglobulina, conjugada à peroxidase, obtida de soro de coelho anti-ConA.
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Publication Dates
-
Publication in this collection
26 June 2009 -
Date of issue
Sept 1986
