Synthesis of 2-(alkylamino)-1-phenylethane-1-thiosulfuric acids, potential schistosomicides

Abstract

The total synthesis of seven here-to-fore unreported aromatic aminoalkanethiosulfuric acids, their physical properties and those of the aminoalcohol and bromoalkanamine intermediates are reported. All structures were established by including ¹H and 13C NMR, IR and MS spectroscopy and elemental analysis.

aminolkanethiosulfate; schistosomicide; beta-aminoalcohol

Synthesis of 2-(alkylamino)-1-phenylethane-1-thiosulfuric acids, potential schistosomicides

Liliani Salum Alves Moreira and Dorila Piló-Veloso

Departamento de Química - Instituto de Ciências Exatas - Universidade Federal de Minas Gerais - Av. António Carlos, 6627 - 31270-901 - Belo Horizonte - MG

David Lee Nelson*

Departamento de Alimentos - Faculdade de Farmácia - Universidade Federal de Minas Gerais - Av. Olegário Maciel, 2360 - Lourdes - 30180-112 - Belo Horizonte - MG

Recebido em 18/2/99; aceito em 25/11/99

The total synthesis of seven here-to-fore unreported aromatic aminoalkanethiosulfuric acids, their physical properties and those of the aminoalcohol and bromoalkanamine intermediates are reported. All structures were established by including ¹H and ¹³C NMR, IR and MS spectroscopy and elemental analysis.

Keywords: aminolkanethiosulfate; schistosomicide; b-aminoalcohol.

INTRODUCTION

Schistosomiasis is an endemic disease which is caused by the parasite Schistosoma mansoni and which affects ten to twelve million persons in Brazil¹.Drugs such as oxamniquine²,preferred for mass treatment, may produce mutagenic effects and cause hepatic lesions; in addition, resistant strains of S. mansoni have been observed^3-5. Thus, studies aimed at the production of new drugs active against this disease have been realized^6-10. Several compounds which have demonstrated activity against this disease can be synthesized via the intermediate b¾aminoalcohols and bromoalkanamines. These substances include the mercaptoalkanamines, the aminoalkanethiosulfuric acids and the corresponding aminoalkyl disulfides^6-8,11-14. An alternate route to the aminoalkanethiosulfuric acids involves conversion of epoxides to thiiranes¹⁵ and the reaction of thiiranes with primary amines to furnish the mercaptoalkanamines¹⁶. The disulfides may be obtained by oxidation of the amino-thiols¹⁴, and they may be converted to the thiosulfuric acids by treatment with sodium sulfite¹². However, because of the ease of polymerization of the thiiranes, the yields obtained in the reaction with the amines are frequently very low, especially when the amine bears a branched alkyl group. Also, the maximum yield obtainable in the reaction of the disulfide with sodium sulfite is necessarily only 50%, based on the thiol from which the disulfide is obtained. Therefore, the route via aminoalcohols is preferred. The aminoalcohols may also be converted to aziridines which can be opened with thiosulfuric acid¹², but the number of steps is the same as in the selected route and the probability of rearrangement is higher because of the acidic conditions employed. The preparation of aziridines using the method of Corey and Chaykovsky¹⁷ has not proved fruitful when the imine bears an aliphatic group¹⁸.

The activity of several aliphatic aminoalkanethiosulfuric acids against schistosomiasis has been demonstrated^6,8-10. However, the effect of an alpha phenyl substituent on the activity of these compounds has never been studied. Therefore, seven new aromatic aminoalkanethiosulfuric acids (4) were synthesized with the objective of verifying their in vivo activities against schistosomiasis. These syntheses involved classical methods as shown in Scheme 1.

RESULTS AND DISCUSSION

The literature describes various synthetic methods for the preparation of b-aminoalcohols, including the substitution reaction of primary and secondary amines with alkylene chlorohydrins^19-20; the reduction of a-hydroxyamides²¹ and the opening of 1,2-epoxyalkanes by primary and secondary amines²². This last method was chosen because of its simplicity and the availability of starting materials. According to the Kraussuski rule²³, the nucleophile attacks the less substituted carbon in a bimolecular substitution mechanism to form the aminoalcohol shown in Scheme 1.

In the synthesis of b-aminoalcohols, Biel²⁴ obtained good yields by refluxing a mixture containing a secondary amine, epoxide and metanol in the molar ratio of 3:1:0.5. With the aim of improving the yields of these reactions, a modification of the procedure was used in which the reaction was carried out under pressure in a Parr reactor. This modification also resulted in a significant reduction in the reaction time. Even though an excess of amine was used, there was still the possibility of obtaining secondary products since primary amines were being used, as well as of polymerization of the epoxide and the products. These factors would be responsible for the relatively low yields for many of the aminoalcohols obtained even with the improvements in the method. No attempt was made to maximize the yields.

The amino groups of the b-aminoalcohols were protected by conversion to the hydrobromide salts and then the hydroxyl groups were substituted by bromide using excess phosphorous tribromide in refluxing benzene, in accord with the method of Leffler and coworkers²⁵. With the present series of aminoalcohols, there was no possibility of rearrangement of the intermediate carbonium ion, as has been observed in previous syntheses⁶.

The b-bromoalkanamines were converted to the Bunte salts by reaction with sodium thiosulfate^26-29. Even under weakly acidic conditions (pH = 3.5), a rearrangement normally occurs in which the b-bromoalkanamines are converted to the intemediate aziridinium ions which then suffer attack on the least hindered carbon to yield the primary thiosulfates (Scheme 2)⁸. Because of the instability of thiosulfuric acid, a lower pH cannot be used. However, in the present case, because of the high stability of the intermediate benzilic carbocation, the preferred pathway involved attack on the benzilic carbon to yield the secondary thiossulfuric acid. HPLC analyses demonstrated the presence of approximately 10% of a secondary product which was no doubt the primary thiosulfuric acid although it was not isolated for identification. Relatively low yields of the final product were obtained when the alkyl group bound to the amine was branched, possibly in part because of a degree of steric hindrance, but chiefly because of difficulty in purifying the products.

The structures of the intermediates and products were confirmed by IR^30-32, NMR^32,33 and mass spectroscopy^32,34. In the ¹H NMR spectra of all the 2-(alkylamino)-1-phenyl-1-ethanols (2), the signal for the amino and hydroxyl hydrogens was observed in the region between d = 3.4 and 3.9. The signal corresponding to the methinyl hydrogen appeared between d = 4.5 and 4.8, whereas that corresponding to the aromatic hydrogens appeared as a sharp peak centered at d = 7.2. The spectra were also determined in CF₃CO₂D in order to observe the effect of protonation of the amino group on the chemical shifts of the neighboring hydrogens for later comparison with the spectra of the bromoalkanamine hydrobromides (3). In the case of 2f, the double doublet expected for the methinyl hydrogen alpha to the hydroxyl group is complicated, probably because of superposition of the signals for the two diasteriomers which are formed in the reaction of the epoxide with the racemic s-butylamine.

The alterations observed in the ¹H NMR spectra of 3, when compared with those of the respective aminoalcohols, are the substitution of the hydroxyl group for a bromine atom and protonation of the amino group which appeared as a wide signal from d = 8¾10. The signal for the methinyl hydrogen is shifted to the d = 5.7¾5.8 region. Although one might expect to see a triplet, as in the case of 3b, a double doublet was observed for the other bromoalkanamines, indicating that the methylenic hydrogens alpha to the ammonium group [PhCH(Br)CH₂NH₂R⁺] are not equivalent.

In 3d, with an 80 MHz instrument, the simple first order spectrum is observed. However, on a 400 MHz instrument, instead of a simple doublet for the gem methyl groups due to coupling with the methinyl proton, two doublets are observed because the two methyl groups are in different environments. Thus, rotation around the C1¾C2 bond is not completely free. In 3e, 3f and 3g, the two hydrogens bound to the nitrogen are in very different environments, leading to very different chemical shifts. This is no doubt due to the steric hindrance to rotation because of the more voluminous alkyl groups bound to the amino group and the large bromine atom. The remaining signals also presented a chemical shift to higher d.

In the spectra of the 2-(alkylamino)-1-phenylethane-1-thiosulfuric acids, all the signals for the alkyl substituents are observed between d = 1.0 and 4.0. The signal for the methinyl hydrogen is found near d = 4.6 as a triplet, with the exception of 4b and 4d, which present a multiplet and a double doublet, respectively.

The signal corresponding to the methylenic hydrogens alpha to the ammonium group is observed as a doublet for 4d, but appears as a multiplet (4a, 4b, 4e and 4f) or two double doublets (4c and 4g) in the case of the remaining compounds. The signal for the ammonium hydrogens appears near d = 8.5 and the chemical shifts for the aromatic hydrogens are similar to those of 2 and 3.

The infrared spectra of the aromatic b-aminoalcohols (2) are quite similar. The bond stretching absorptions of the NH and OH groups are observed between 3400 to 3100 cm^-1 and the angular deformation absorption of the NH group is observed at 1650 cm^-1, as well as the out of plane bending absorptions of the C¾OH bond near 650 cm^-1. The absorption resulting from the C¾O stretching vibration is found between 1180 and 1050 cm^-1.

The IR spectra of the bromoalkanamines (3) show the bands characteristic of the ammonium stretching vibrations between 2400 and 2550 cm^-1 and the bending vibration at 1580 cm^-1. Evidence for the C-Br bond is presented by a weak absorption beyond 500 cm^-1. The IR spectra of (4) present absorption bands between 510 and 550 cm^-1, attributed to the SSO₃^- group and the symmetrical and unsymmetrical stretching bands of the SO₃^- and S-S bonds are observed at 620, 1020 and 1180 cm^-1. In addition, they show one band between 2360 and 2462 cm^-1 which is not a fundamental band and is identified as a combination band associated with the zwitterion structure^31,32.

The decoupled ¹³C NMR spectra of 2 present signals characteristic of the aromatic carbons between d = 125¾144, a signal for CHOH in the region between d = 71¾72 and a signal for CH₂NHR in the region between d = 54¾57. The signals attributed to the carbons of 2, 3 and 4 were compared with those calculated using the ACD/CNMR version 1.0 simulation program and a good agreement was obtained. The signals for the benzilic carbons of 4 varied from d = 47 to 49 and for the neighboring methylene carbons from d = 50 to 53.

The mass spectra of 4 all showed similar fragmentation patterns: a peak resulting from fragmentation beta to the aromatic ring (m/z = 147 Thonson for R = C₃H₇; 161 Thonson for R = C₄H₉; 187 Thonson for R = C₆H₁₁) and a peak corresponding to the tropylium ion at m/z = 92 Thonson. As has been previously observed¹⁵, since the thiosulfate decomposes when heated, the molecular ion and fragmentation peaks observed are chiefly those of the corresponding disulfides (m/z = 388, 416 and 468 Thonson) and thiols (m/z = 195, 209 and 235 Thonson) rather than those of the thiosulfuric acids.

EXPERIMENTAL

General Remarks: Melting points were determined on a Mettler FP 80 HT melting point apparatus and are uncorrected. Infrared spectra were registered on Shimadzu IR 408 and Mattson Instruments FTIR spectrophotometers in KBr disks. ¹H and ¹³C NMR spectra were recorded on Bruker AC-80, Bruker DPX200 and Bruker DRX400 AVANCE spectrometers. Chemical shifts are reported in parts per million (d units) relative to tetramethylsilane or CDCl₃ as internal standards. Coupling constants (J) are reported in Hertz. The numbering of the carbons of the alkyl groups in the assignment of the chemical shifts of the ¹³C NMR spectra of 2 and 4 was systematic, as illustrated below for the sec-butyl derivative (^{Structure 2f/4f}). Mass spectra of the b-aminoalcohols (2) were determined on a Hewlett-Packard Model HP-5989A GC-MS monoquadropole spectrometer equipped with an HP-5ME capillary column. Mass spectra of the aminoalkanethiosulfuric acids (4) were determined by introduction of solid via probe into a GCQ Finnigan GC-MS spectrometer equipped with an ion trap detector. The ionization energy used was 70 eV in all cases. Isobutane was used for chemical ionization. Elemental analyses were obtained on a Perkin-Elmer Model 2400 apparatus. All reagents were reagent grade. Amines were treated with KOH for 24 hr, filtered and distilled.

General Procedure for the Synthesis of 2-Alkylamino-1-phenyl-1-ethanol 2: A mixture of 0.125 moles of styrene oxide, 0.6 moles of methanol and 0.376 moles of alkylamine were stirred in a Parr reactor for 6 hr at 120^oC under a pressure of 60 psig. The reactor was cooled to room temperature, and the methanol and excess amine were removed on a rotary evaporator. The residue was recrystallized from petroleum ether.

2a: Yield = 11.2 g (50%); m.p.: 57¾9^oC; IR (KBr): n_max 3300, 3100, 2950, 2850, 2750, 1650, 1450, 1430, 1400, 1350, 1110, 1070, 1050, 1030, 940, 860, 750, 700, 650, 550, 520, 450 cm^-1; ¹H NMR (CDCl₃, 200 MHz): d 0.91 (t, J = 7.3 Hz, 3 H, CH₃), 1.49 (sex, J = 7.3 Hz, 2 H, MeCH₂CH₂), 2.52¾2.67 (m, 2 H, EtCH₂NH), 2.69 (dd, J_ab = 9.0, 12.1 Hz, 1 H CHCH_aH_bN), 2.85 (dd, J = 3.8, 12.1 Hz, 1 H, CHCH_aH_bN), 4.71 (dd, J = 3.8, 9.0 Hz, 1 H, CHOH), 7.25¾7.38 (m, 5 H, aromatic CH); ¹³C NMR (CDCl₃, 50 MHz): d 142.8 (C_ipso), 128.3 (o-CH), 127.4 (p-CH), 125.8 (m-CH), 76.4 (CH-1), 56.9 (CH₂-2), 51.2 (CH₂-1´), 22.7 (CH₂-2´), 11.5 (CH₃-3´); MS m/z (%): 194 (4), 176 (2), 132 (6), 105 (5), 86 (100); 79 (7), 77 (11), 57 (16); C₁₁H₁₇NO (179): calcd. C 73.70%, H 9.56%, N 7.81%; found C 73.63%, H 9.39%, N 7.64%.

2b: Yield = 9.2 g (41%); m.p.: 85¾7^oC; IR (KBr): n_max 3300, 3050, 2950, 2800, 2750, 1605, 1230, 1180, 1140, 1000, 1060, 1030, 985, 955, 925, 915, 880, 800, 750, 700, 670 cm^-1; ¹H NMR (CDCl₃, 80 MHz): d 0.99 (d, J = 6.2 Hz, 6 H, CH₃), 2.61¾2.91 (m, 3 H, Me₂CH, CH₂NH), 3.34 (broad, 2 H, NHR, OH), 4.70 (dd, J = 8.6, 4.2 Hz, 1 H, CHOH), 7.30 (s, 5 H, aromatic CH); ¹³C NMR (CDCl₃, 20 MHz): d 143.1 (C_ipso), 128.2 (o-CH), 127.3 (p-CH), 125.8 (m-CH), 72.0 (CH-1), 54.7 (CH₂-2), 48.6 (CH-2´), 22.9 (CH₃-1´); MS m/z (%): 180 (7), 162 (2), 146 (3), 105 (2), 79 (6), 77 (10), 72 (100); C₁₁H₁₇NO (179): calcd. C 73.70%, H 9.56%, N 7.81%; found C 73.71%, H 11.34%, N 7.82%.

2c: Yield = 10.1 g (42%); m.p: 59¾60^oC; IR(KBr): n_max 3300, 3050, 2950, 2820, 1460, 1440, 1350, 1125, 1090, 1080, 1065, 1040, 960, 920, 870, 760, 700, 670 cm-¹; ¹H NMR (CDCl₃, 80 MHz): d 0.83 (t, J = 5.9 Hz, 3 H, CH₃), 1.09 ¾ 1,30 (m, 4 H, MeCH₂CH₂CH ₂), 2.47 (t, J = 6.9 Hz, 2 H, PrCH₂), 2.64 (d, J = 6.4 Hz, 2 H, CHCH₂N), 3.81(broad, 1 H, NHR), 4.74 (t, J = 6.4 Hz, 1 H, CHOH), 7.24 (s, 5 H, aromatic CH); ¹³C NMR (CDCl₃, 20 MHz): d 143.1 (C_ipso), 128.2 (o-CH), 127.3 (p-CH), 125.8 (m-CH), 71.7 (CH-1), 57.2 (CH₂-2), 49.2 (CH₂-1´), 32.0 (CH₂-2´), 20.3 (CH₂-3´), 13.9 (CH₃-4´); MS m/z (%): 194 (47), 176 (8), 150 (1), 132 (3), 105 (4), 86 (100), 79 (11), 77 (13), 57 (6); C₁₂H₁₉NO (193): calcd. C 74.57%, H 9.91%, N 7.25%; found C 74.72%, H 12.0%, N 7.27%.

2d: Yield = 8.9 g (37%); m.p.: 62¾3^oC; IR (KBr): n_max 3330, 3100, 2980, 2820, 2730, 2560, 1650, 1450, 1430, 1125, 1100, 1060, 1050, 990, 940, 890, 820, 760, 700, 650, 550, 540, 470 cm-¹; ¹H NMR (CDCl₃, 80 MHz): d 0.83 (d, J = 6.5 Hz, 6 H, CH₃), 1.66 (hep, J = 6.5 Hz, 1 H, Me₂CH), 2.33 (d, J = 6.6 Hz, 2 H, i-PrCH₂N), 2.66 (d, J = 6.3 Hz, 2 H, CHCH₂N), 3.44 (broad, 1 H, NHR), 4.70 (t, J = 6.3 Hz, 1 H, CHOH), 7.27 (s, 5 H, aromatic CH); ¹³C NMR (CDCl₃, 20 MHz): d 142.8 (C_ipso), 128.3 (o-CH), 127.3 (p-CH), 125.8 (m-CH), 71.6 (CH-1), 57.2 (CH₂-2), 57.2 (CH₂-1´), 28.3 (CH-2´), 20.5 (CH₃-3´); MS m/z (%): 194 (4), 176 (2), 132 (6), 105 (5), 86 (100), 79 (7), 77 (11), 57 (16); C₁₂H₁₉NO (193): calcd. C 74.57%, H 9.91%, N, 7.25%; found C 74.68%, H 12.88%, N 7.21%.

2e: Yield = 20.5 g (85%); m.p.: 86¾8^oC; IR (KBr): n_max 3330, 3100, 2980, 2850, 2550, 1600, 1450, 1360, 1340, 1240, 1090, 1050, 1020, 960, 920, 860, 750, 700, 630, 560, 520, 470, 440 cm^-1; ¹H NMR (CDCl₃, 80 MHz): d 1.04 [s, 9 H, C(CH₃)₃], 2.46¾2.88 (m, 3 H, CH₂N), 4.66 [dd, J = 6.6, 4.4 Hz, 1 H, CHOH], 7.30 [s, 5 H, aromatic CH]; ¹³C NMR (CDCl₃, 20 MHz): d 143.1 (C_ipso), 128.2 (o-CH), 127.3 (p-CH), 125.8 (m-CH), 72.3 (CH-1), 50.2 (CH₂-2), 50.2 (C-2´), 29.1 (CH₃); MS m/z (%): 194 (8), 178 (1), 160 (13), 86 (100), 77 (15), 57 (18); C₁₂H₁₉NO (193): calcd. C 74.57%, H 9.91%; N 7.25%; found C 74.76%, H 11.47%, N 7.35%.

2f: Yield = 15.7 g (65%); p.e.: 150^oC (1.5 mm Hg); IR.(KBr): n_max 3300, 3100, 2970, 2390, 1660, 1450, 1400, 1360, 1220, 1100, 1070, 1030, 940, 870, 770, 700, 640 cm^-1; ¹H NMR (CDCl₃, 200 MHz): d 0.81 (t, J = 7.4 Hz, 3 H, CH₂CH₃), 0.95 [d, J = 6.3 Hz, 3 H, CHCH₃ (diasteriomer a)], 0.96 [d, J = 6.3 Hz, 3 H, CHCH₃ (diasteriomer b)], 1.10 ¾ 1.56 (m, 2 H, CH₂Me), 2.44¾2.84 (m, 3 H, CH₂NHCH), 3.70 (broad s, 2 H, NHR, OH), 4.66 ¾ 4.78 (m, 1 H, CHOH), 7.31 (m, 5 H, ring CH); ¹³C NMR (CDCl₃, 50 MHz): d 143.1 (C_ipso), 128.2 (o-CH), 127.2 (p-CH), 125.7 (m-CH), 71.8 (CH-1), 54.6 (CH-2´), 54.2 (CH₂-2), 29.5 (CH₂-3´), 19.6 (CH₃-1´), 10.1 (CH₃-4´); MS m/z (%): 194 (100), 176 (6), 146 (4), 107 (4), 79 (18), 77 (19), 57 (23); C₁₂H₁₉NO (193): calcd. C 74.61%, H 9.85%, N 7.25%; found C 77.14%, H 11.49%, N 6.43%.

2g: Yield = 10.7 g (39%); m.p.: 91¾2^oC. IR (KBr): n_max 3250, 3080, 2900, 2850, 2800, 1610, 1500, 1450, 1380, 1345, 1220, 1120, 1080, 1065, 1030, 980, 940, 890, 880, 750, 700, 665 cm^-1; ¹H NMR (CDCl₃, 200 MHz): d 0.90¾1.35 (m, 5 H, ring CH₂), 1.50¾1.80 (m, 3 H, ring CH₂), 1.80¾1.93 (m, 2 H, ring CH₂), 2.42 (tt, J = 3.6, 10.1 Hz, 1 H, ring CHN], 2.66 [dd, J = 9.1, 12 Hz, 1 H, CHCH_aH_bNH], 2.94 [dd, J = 3.7, 12 Hz, 1 H, CHCH_aH_bN], 4.65 [dd, J = 3.7, 9.1, 1 H, CHOH], 7.22¾7.37 [m, 5 H, aromatic CH]; ¹³C NMR (CDCl₃, 50 MHz): d 143.0 (C_ipso), 128.3 (o-CH), 127.3 (p-CH), 125.8 (m-CH), 71.7 (CH-1), 56.5 (CH-1´), 54.3 (CH₂-2), 33.3 [CH₂-2´(6´)], 25.9 (CH₂-4´), 24.9 [CH₂-3´(5´)]; MS m/z (%): 220 (28), 202 (3), 112 (100), 79 (13), 55 (14); C₁₄H₂₁NO (219): calcd. C 76.67%, H 9.65%, N 6.39%; found C 76.54%, H 9.81%, N 6.15%.

General Procedure for the Synthesis of N-Alkyl-1-bromo-1-phenyl-2-ethanamine hydrobromide (3): To a stirred solution of 0.1 moles of 2-(alkylamino)-1-phenyl-1-ethanol (2) in 50 mL of ethanol, was added 0.11 moles of 48% hydrobromic acid over a one hour period at ice bath temperature. The ethanol and excess hydrobromic acid were removed on a rotary evaporator and the crude product was dried under vacuum. Addition of dry ethanol to the yellow solid resulted in precipitation of a white solid which was dried in a desiccator.

To a stirred mixture of the b-aminoalcohol hydrobromide and 120 mL of dry benzene, 7.5 mL (0.15 moles) of redistilled PBr₃ was slowly added. The system was refluxed for 4 hr, the course of the reaction being accompanied by TLC. The mixture was allowed to stand for 24 hr and the benzene and excess PBr₃ were removed on a rotary evaporator. The residue was recrystallized from AcOEt:i-PrOH (9:1).

3a: Yield = 26.3 g (81.4%); m.p.: 194¾5^oC; IR (KBr): n_max 3425, 2960, 2800, 2400, 1563, 1438, 1392, 1367, 1233, 1183, 1008, 983, 763, 700, 583, 517, 467 cm^-1; ¹H NMR (CDCl₃, 200 MHz): d 0.99 (t, J = 7.4 Hz, 3 H, CH₃), 1.95 (sex, J = 7.5 Hz, 2 H, CH₂CH₂Me), 2.93¾3.20 (m, 2 H, CH₂Et), 3.61 (dd, J = 5.6, 13.5 Hz, 1 H, CHCH_aH_bN), 3.73 (dd, J = 9.0, 13.5 Hz, 1 H, CHCH_aH_bN), 5.73 (dd, J = 5.6, 9.0, 1 H, CHBr), 7.36¾7.41 (m, 3 H, m- and p-aromatic CH), 7.44¾7.53 (m, 2 H, o-aromatic CH), 8.98 ¾ 9.30 (broad, 2 H, NH₂⁺); ¹³C NMR (CDCl₃, 50 MHz ): d 137.4, 129.6, 129.2, 127.7, 54.0, 49.9, 47.1, 19.2, 11.2.

3b: Yield = 18 g (56%); m.p.: 172¾4^oC; IR (KBr,): n_max 3425, 2930, 2750, 2375, 1575, 1475, 1450, 1400, 1375, 1290, 1150, 1000, 775, 705, 600, 538, 475 cm^-1; ¹H NMR (CDCl₃, 400 MHz): d 1.50 [dd, J = 6.6, 10.3 Hz, 3 H, CH(CH₃)₂], 1.55 [dd, J = 6.6, 10.3 Hz, 3H, CH(CH₃)₂], 3.57¾3.71 [m, 3 H, Me₂CH, CHCH₂N], 5.78 (t, 1 H, 7.1, CHBr), 7.36-7.55 (m, 5 H, aromatic CH); ¹³C NMR (MeOH, 50 MHz): d 139.1, 130.7, 130.3, 128.9, 53.1, 52.5, 48.9, 19.3, 18.7.

3c: Yield = 22.6 g (67%); m.p.: 153¾5^oC; IR(KBr): n_max 3475, 2950, 2790, 2450, 1605, 1450, 1400, 1250, 1188, 775, 700, 600, 525, 487 cm^-1; ¹H NMR (CDCl₃, 400 MHz): d 0.93 (t, J = 7.3 Hz, 3 H, CH₃), 1.40 (sex, J = 7.5 Hz, 2 H, CH₂CH₂Me), 1.90 (quin, J = 7.8 Hz, 2 H, CH₂CH₂CH₂), 2.99¾3.15 (m, 2 H, PrCH₂N), 3.62 (dd, J = 5.3, 13.5 Hz, 1 H, CHCH_aH_bN), 3.75 (dd, J = 9.3, 13.5 Hz, 1 H, CHCH_aH_bN), 5.75 (dd, J = 5.3, 9.3 Hz, 1 H, CHBr), 7.36 ¾ 7.41 (m, 3 H, m- and p-aromatic CH), 7.50¾7.53 (m, 2 H, o-aromatic CH ), 9.03¾9.46 (broad, 2 H, NH₂⁺); ¹³C NMR (CDCl₃, 100 MHz): d 137.6, 129.6, 129.3, 127.7, 54.0, 48.3, 47.2, 27.6, 20.0, 13.5.

3d: Yield = 21.9 g (65%); m.p.: 143¾4^oC; IR (KBr): n_max 3400, 2960, 2775, 2330, 1538, 1475, 1437, 1413, 1213, 900, 763, 700, 575, 513, 488 cm^-1; ¹H NMR (CDCl₃, 400 MHz): d 1.05 [d, J = 6.7 Hz, 3 H, (CH₃)_aCH(CH₃ )_b], 1.08 [d, J = 6.7 Hz, 3 H, (CH₃)_aCH(CH₃ )_b], 2.24 (hep, J = 6.8 Hz, 1 H, Me₂CH), 2.80 (dd, J = 7.5, 12.4 Hz, 1 H, i-PrCH_aH_bN), 2.95 (dd, J = 6.8, 12.4 Hz, 1 H, i-PrCH_aH_bN), 3.68 (dd, J = 5.6, 13.6 Hz, 1 H, CHCH_aH_bN), 3.73 [dd, J = 9.0, 13.6 Hz, 1 H, CHCH_aH_bN), 5.82 [dd, J = 5.6, 9.0 Hz, 1H, CHBr], 7.27¾7.41 [m, 3 H, m- and p-aromatic CH], 7.47¾7.54 [m, 2 H, o-aromatic CH], 9.05 [broad, 2 H, NH₂⁺]; ¹³C NMR (CDCl₃, 100 MHz): d 137.6, 129.7, 129.3, 127.8, 55.3, 54.3, 47.1, 25.6, 20.5.

3e: Yield = 21.9 g (65%); m.p.: 168¾9^oC; IR (KBr): n_max 3425, 3025, 2775, 2380, 1562, 1488, 1475, 1375, 1250, 1200, 1169, 1063, 1000, 963, 769, 700, 575, 526, 431 cm^-1; ¹H NMR (CDCl₃, 400 MHz): d 1.60 [s, 9 H, C(CH₃)₃], 3.48¾3.72 (m, 2 H, CHCH₂N), 5.82 (dd, J = 4.0, 11.5 Hz, 1 H, CHBr), 7.35¾7.43 (m, 3 H, m- and p-aromatic CH), 7.51 ¾ 7.56 (m, 2 H, o-aromatic CH ), 7.80 and 9.95 (broad, 1 H each, NH_aH_b⁺); ¹³C NMR (CDCl₃, 100 MHz ): d 137.5, 129.5, 129.2, 127.6, 59.4, 50.2, 48.4, 26.2.

3f: Yield = 4%; m.p.: 144¾5^oC; IR (KBr): n_max 3460, 2961, 2756, 2420, 1577, 1456, 1412, 1387, 1281, 1244, 1113, 763, 699, 595, 507 cm^-1; ¹H NMR (CDCl₃, 400 MHz): Less abundant diasteriomer: d 0.99 (t, J = 7.4 Hz, 3 H, CH₂CH₃), 1.51 [d, J = 6.6 Hz, 3 H, CH(CH₃)], 1.68 ¾ 1.83 (m, 2 H, MeCH₂), 3.42¾3.48 [m, 1 H, CH(Me)Et], 3.59¾3.72 (m, 2 H, CHCH₂N); More abundant diasteriomer: d 1.0 (t, J = 7.5 Hz, 3 H, CH₂CH₃), 1.45 [d, J = 6.7 Hz, 3 H, CH(CH₃)], 1.99¾2.14 (m, 2 H, MeCH₂), 3.32¾3.38 [m, 1 H, CH(Me)Et], 3.59¾3.72 (m, 2 H, CHBrCH₂); Both isomers: d = 5.81¾5.87 (m, 1 H, CHBr), 7.33¾7.40 (m, 3 H, m- and p-aromatic CH), 7.51¾7.55 (m, 2 H, o-aromatic CH), 8.61¾8.76 and 9.42¾9.57 (broad, 1 H each, NH_aH_b⁺); ¹³C NMR (CDCl₃, 100 MHz): d 137.5, 129.5, 129.1, 127.6, 56.3, 50.6, 47.3, 25.9, 15.3, 10.2.

3g: Yield = 23.6 g (65%); m.p.: 158¾9^oC; IR (KBr): n_max 3425, 2950, 2719, 2425, 1588, 1463, 1238, 1050, 1013, 775, 700, 600, 538, 482 cm^-1; ¹H NMR (CDCl₃, 400 MHz ): d 1.10¾1.31 (m, 3 H, ring CH₂ ), 1.55¾1.86 (m, 6 H, ring CH₂), 2.17¾2.35 (m, 2 H, ring CH₂), 3.15 (tt, J = 3.6, 11.6 Hz, 1 H, ring CH), 3.66 (dd, J = 8.8, 13.6 Hz, 1 H, CHCH_aH_bN), 3.73 (dd, J = 5.5, 13.6 Hz, 1 H, CHCH_aH_bN), 5.81 (dd, J = 5.5, 8.8 Hz, 1 H, CHBr), 7.36¾7.38 (m, 3 H, m- and p-aromatic CH), 7.50¾7.53 (m, 2 H, o-aromatic CH), 8.68 and 9.48 (broad, 1 H each, NH_aH_b⁺); ¹³C NMR (CDCl₃, 100 MHz): d 139.9, 129.2, 128.7, 125.9, 68.9, 58.7, 51.0, 29.2, 24.5.

General Procedure for the Synthesis of 2-(alkylamino)-1-phenylethane-1-thiosulfuric acid (4): A solution containing 0.02 moles of (3) dissolved in the minimum volume necessary of 50% ethanol was stirred with an equimolar quantity of a concentrated aqueous solution of sodium thiosulfate for 2 hr at room temperature. The reaction was monitored by TLC using ethyl acetate:chloroform:methanol [1:2:0.5] as eluent and its completion was determined by the lack of a precipitate of sulfur when sulfuric acid was added to an aliquot of the reaction mixture. After completion of the reaction, the mixture was filtered and the resulting white solid was recrystallized from chloroform and dried in a desiccator.

4a: Yield = 4.0 g (73%); m.p.: 198¾9^oC; IR (KBr): n_max 3451, 3026, 2363, 1583, 1454, 1210, 1023, 630, 538 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 0.88 [t, J = 7.1 Hz, 3H, CH₃], 1.54¾1.65 [m, 2 H, CH₂CH₂CH₃], 2.86¾2.93 [t, J = 7.3 Hz, 2 H, CH₂Et], 3.56 [dd, J = 6.9, 12.5, 1 H, PhCHCH_aH_bNH₂ ⁺]; 3.77 [dd, J = 7.3, 12.5, 1 H, PhCHCH_aH_bNH₂ ⁺] 4.65 [t, J = 7.2 Hz, 1 H, PhCHSSO₃^-], 7.35 [s, 5 H, aromatic CH], 8.47 [broad, 2 H, NH₂R⁺]; ¹³C NMR (DMSO-d₆, 50 MHz): d 138.4 (C_ipso), 129.0 (p-CH), 128.0 (o-CH), 127.6 (m-CH), 52.2 (CH₂-2), 49.1 (CH₂-1´), 48.6 (CH-1), 18.9 (CH₂-2´), 10.8 (CH₃-3´); MS (EI) m/z (%): 39 (10), 41 (16), 43 (8), 55 (20), 56 (7), 57 (8), 67 (6), 69 (5), 70 (12), 71 (7), 82 (5), 83 (15), 84 (6), 100 (52) [H₂SO₃ + H₂O]⁺, 101 (1), 111 (41) [CH₃NHSO₂H]⁺, 112 (7), 128 (7), 129 (100) [CH₃NHSO₂H + H₂O]⁺, 130 (7), 146 (5), 147 (52) [PhCHCH₂NC₂H₅] ⁺, 149 (6), 241 (10) [PhCH (CH₂ NHC₃H₇)SSH=CH₂] ⁺, 259 (16) [PhCH(CH₂N=CHCH₃) SSO₃]⁺, 386 (2) [{PhCH(CH=NHC₃H₇)S}₂ ]⁺. MS (CI) m/z 137 (6), 162 (18), 192 (8), 194 (5), 196 (13), 233 (7), 236 (6), 293 (18), 294 (11), 295 (100), 296 (44), 297 (12), 298 (5), 324 (6), 327 (6), 328 (6), 389 (4) [{PhCH(CH₂NHC₃H₇)S} ₂ + H]⁺; C₁₁H₁₇NO₃S₂ (275.4): calcd. C 48.0%, H 6.18%, N 5.09%, S 23.27%; found C 48.07%, H 6.30%, N 4.77%, S 25.52%.

4b: Yield = 1.8 g (33%): m.p.: 175¾7^oC. IR (KBr): n_max 3445, 3052, 2369, 1583, 1454, 1244, 1195, 1023, 759, 630, 538 cm^-1.; ¹H NMR (DMSO-d₆, 200 MHz): d 1.58 [s, 6 H, CH(CH₃)₂], 3.36¾3.81 (m, 3 H, CH₂NH₂⁺, Me₂CH), 4.51¾4.66 (m, 1 H, PhCHSSO₃^-), 7.39¾7.41 (s, 5 H, aromatic CH), 8.43 (broad, 2 H, NH₂R⁺).; ¹³C NMR (DMSO-d₆, 50 MHz): d = 138.3 (C_ipso), 128.9 (p-CH), 128.4 (o-CH), 127.9 (m-CH), 50.5 (CH₂-2), 48.8 (CH-2´), 46.6 (CH-1), 18.5 (CH₃-1´); MS (EI) m/z (%): 39 (29), 41 (35), 55 (21), 64 (30), 72 (40) [CH₂=NHC₃H₇]⁺ , 77 (42) [Ph]⁺, 91 (93) [C₇H₇]⁺, 104 (68), 118 (45), 121 (73), 135 (64), 146 (53) [PhCHCH₂NC₂H₄] ⁺, 161 (26) [PhCHCH₂NC₃H₇] ⁺, 162 (51) [PhCHCH₂NHC₃H₇] ⁺, 176 (100) [PhThiazoleCH₃]⁺, 192 (36), 227 (28), 241 (16), 250 (47), 295 (34), 309 (33), 387 (6) [{PhCH(CH₂NHC₃H₇)S} ₂ - H]⁺. ¾ MS (CI) m/z (%) 162 (49), 164 (11), 192 (15), 194 (11), 196 (22) [PhCH(CH₂NHC₄H₉)SH + H]⁺, 293 (42), 294 (15), 296 (20), 387 (10), 389 (100) [{PhCH(CH₂NHC₃H₇)S} ₂ + H]⁺, 390 (22); C₁₁H₁₇NO₃S₂ (275.4): calcd. C 48.0%, H 6.18%, N 5.09%; S 23.27%; found C 46.57, H 6.26%, N 4.19%, S 25.52.

4c: Yield = 2.72 g (47%); m.p.: 175¾6^oC; IR (KBr): n_max 3438, 2955, 2363, 1461, 1223, 1023, 759, 630, 539 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 0.87 (t, J = 7.2 Hz, 3 H, CH₃), 1.21¾1.39 (sex, J = 7.2 Hz, 2 H, CH₂CH₂CH₃), 1.49¾1.63 (quin, J = 7.4 Hz, 2 H, CH₂CH₂Et), 2.93 (t, J = 7.3 Hz, 2 H, PrCH₂N), 3.56 (dd, J = 6.8, 12.9 Hz, 1 H, CH_aH_bNH₂ ⁺), 3.77 (dd, J = 8.0, 12.9 Hz, 1 H, CH_aH_bNH₂ ⁺), 4.64 (t, J = 7.3 Hz, 1 H, PhCHSSO₃^-), 7.31 (s, 5 H, aromatic CH), 8.46 (broad, 2 H, NH₂⁺); ¹³C NMR (DMSO-d₆, 50 MHz): d 138.5 (C_ipso), 128.9 (p-CH), 128.0 (o-CH), 127.6 (m-CH), 52.2 (CH₂-2), 48.6 (CH-1), 47.2 (CH₂-1´), 27.3 (CH₂-2´), 19.2 (CH₂-3´), 13.4 (CH₃-4´); MS (EI) m/z (%): 44 (55), 65 (22), 77 (27) [Ph]⁺, 91 (47), 104 (40), 121 (43), 135 (32), 162 (99) [PhCHCH₂ NHC₃H₇]⁺, 176 (51) [PhCHCH₂NHC₄H₉] ⁺, 209 (25) [PhCHSH (CH₂NHC₄ H₉)]⁺, 218 (100) [PhCHCH₃SSO₃H]⁺, 220 (76), 241 (23) [PhCH(CH₂NHC₄H₉)SSH] ⁺, 261 (22), 276 (41), 309 (81), 341 (63); MS (CI) m/z (%) 176 (13), 210 (10) [PhCHSH (CH₂NHC₄H₉) + H]⁺, 261 (6), 262 (9), 306 (5), 308 (79), 309 (100), 310 (37), 311 (13), 312 (7), 341 (20), 342 (21), 343 (6), 344 (5), 346 (6), 415 (7), 417 (64) [{PhCH(CH₂NHC₄H₉)S} ₂ + H]⁺, 418 (16), 419 (7); C₁₂H₁₉NO₃S₂ (289.4): calcd. C 49.8%, H 6.57%, N 4.84%, S 22.15%; found C 49.33%, H 6.67%, N 4.63%, S 22.76%.

4d: Yield = 2.0 g (35%), m.p.: 220¾1^oC; IR (KBr): n_max 3432, 2968, 2385, 1583, 1454, 1203, 753, 624, 532 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 0.92 [d, J = 6.6 Hz, 6 H, CH(CH₃)₂], 1.96 [hep, J = 6.7 Hz, 1 H, Me₂CH], 2.82 (d, J = 5.6 Hz, 2 H, i-PrCH₂N), 3.53 (m, 2 H, CH₂NH₂), 4.70 (dd, J = 5.9, 8.2 Hz, 1 H, PhCHSSO₃^-), 7.35 (s, 5 H, aromatic CH), 8.47 (broad, 2 H, NH₂⁺); ¹³C NMR (DMSO-d₆, 50 MHz): d 138.6 (C_ipso), 130.0 (p-CH), 128.0 (o-CH), 127.5 (m-CH), 54.5 (CH₂-1´), 53.2 (CH₂-2), 48.6 (CH-1), 25.3 (CH-2´), 19.8 (CH₃-3´); MS (EI) m/z (%): 39 (41), 41 (60), 55 (51), 67 (32), 81 (30), 83 (28), 91 (26) [C₇H₇]⁺, 98 (32), 100 (100) [H₂SO₃ + H₂O]⁺, 129 (40), 132 (29), 178 (25), 185 (21), 256 (21); MS (EI) m/z (%) 162 (8), 176 (8), 210 (7) [PhCHSH (CH₂NHC₄H₉) + H]⁺, 243 (12), 250 (23), 261 (11), 277 (17), 307 (23), 308 (24), 309 (100), 310 (45), 311 (12), 337 (10), 417 (2) [{PhCH(CH₂NHC₄H₉)S} ₂ + H]⁺; C₁₂H₁₉NO₃S₂ (289.4): calcd. C 49.8%, H 6.57%, N 4.84%; S 22.15%; found C 49.31%, H 6.54%, N 4.95%, S 22.47%.

4e: Yield = 4.2 g (73%); m.p.: 208¾9^oC; IR (KBr): n_max 3438, 3032, 2462, 1605, 1384, 1249, 1022, 758, 629, 537 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 1.30 [s, 9 H, C(CH₃)₃], 3.40-3.60 (m, 1 H, CH_aH_bNH₂ ⁺); 3.80-4.00 (m, 1 H, CH_aH_bNH₂ ⁺); 4.57 (t, 1H, 7.0, PhCHSSO₃^-), 7.38 (s, 5 H, aromatic CH), 8.42 and 8.62 (broad, 1 H each, NH_aH_b⁺); ¹³C NMR (DMSO- d₆, 50 MHz): d 138.2 (C_ipso), 129.0 (o-CH), 128.1 (p-CH), 127.7 (m-CH), 57.1 (C-2´), 49.5 (CH₂-2), 47.0 (CH-1), 25.1 (CH₃); MS (EI) m/z (%): 39 (77), 41 (100), 43 (69), 45 (40), 55 (85), 67 (65), 69 (44), 73 (41), 77 (39) [Ph]⁺, 79 (49), 81 (64), 83 (47), 87 (46), 91 (96) [C₇H₇]⁺, 95 (50), 96 (41), 98 (40), 100 (82) [H₂SO₃ + H₂O]⁺, 118 (73), 121 (55), 129 (55), 134 (39), 149 (42), 157 (30), 161 (28), 162 (42), 171 (25), 174 (49), 195 (37), 199 (22), 207 (31), 213 (21), 258 (42); MS (CI) m/z (%) 120 (38), 137 (32), 161 (11), 162 (37), 175 (12), 176 (46) [PhCHCH₂NHC₄H₉] ⁺, 192 (12), 193 (12), 194 (16), 196 (25), 206 (17), 207 (16), 208 (25), 210 (42) [PhCH(CH₂NHC₄H₉) SH + H]⁺, 236 (14), 250 (13), 252 (12), 253 (10), 276 (11), 293 (61), 294 (34), 295 (100), 296 (44), 297 (13), 307 (35), 308 (33), 309 (37), 310 (16), 316 (7), 338 (7), 389 (9), 417 (8) [{PhCH(CH₂NHC₄H₉)S} ₂ + H]⁺; C₁₂H₁₉NO₃S₂ (289.4): calcd. C 49.8%, H 6.57%, N 4.84%, S 22.15%; found C 50.34%, H 7.18%, N 9.46%, S 18.34%.

4f: Yield = 1.7 g (30%); m.p.: 173¾6^oC; IR (KBr): n_max 3412, 2981, 2363, 1615, 1454, 1244, 1182, 1022, 771, 633, 542 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 0.89 (t, J = 7.2 Hz, 3 H, CH₃), 1.21 [d, J = 6.2 Hz, 3 H, NCH(CH₃)Et], 1.40¾1.54 (m, 1 H, CH_aH_bMe), 1.67¾1.73 (m, 1 H, CH_aH_bMe), 3.05-3.28 [m, 1 H, NCH(Me)Et], 3.50¾3.68 (m, 1 H, CH_aH_bNH₂ ⁺); 3.68-3.87 (m, 1 H, CH_aH_bNH₂ ⁺); 4.63 [t, J = 6.7 Hz, 1 H, PhCHSSO₃^-], 7.36 [s, 5 H, aromatic CH], 8.44 [broad, 2 H, NH₂⁺]; ¹³C NMR (DMSO-d₆, 50 MHz): d 138.4 (C_ipso), 129.0 (p-CH), 128.1 (o-CH), 127.6 (m-CH), 55.7 (CH-2´), 49.9 (CH₂-2), 49.0 (CH-1), 25.2 (CH₂-3´), 15.3 (CH₃-1´), 9.5 (CH₃-4´); MS (EI) m/z (%): 41 (37), 43 (40), 55 (32), 57 (40), 65 (37), 77 (46) [Ph]⁺, 78 (38), 86 (85), 88 (57), 91 (78) [C₇H₇]⁺, 103 (57), 104 (57), 118 (39), 120 (78), 121 (61), 135 (60), 146 (41), 162 (38), 176 (100) [PhCHCH₂NHC₄H₉] ⁺, 205 (35), 209 (58), 241 (33), 253 (35), 309 (77), 331 (26), 387 (15); MS (CI) m/z (%) 137 (11), 175 (15), 176 (97), 177 (20), 205 (13), 206 (26), 207 (10), 208 (16), 210 (100) [PhCH(NHC₄H₉)CH₂SH + H]⁺, 211 (14), 253 (16), 307 (30), 308 (18), 309 (34), 310 (17), 417 (19) [{PhCH(NHC₄H₉)CH₂S} ₂ + H]⁺; C₁₂H₁₉NO₃S₂ (289.4): calcd. C 49.8%, H 6.57%, N 4.84%, S 22.15%; found C 49.71%, H 6.77%, N 5.19%, S 21.99%.

4g: Yield = 4.4 g (70%); m.p.: 193¾5^oC; IR (KBr): n_max 3451, 3013, 2409, 1590, 1448, 1242, 1016, 761, 624, 532 cm^-1; ¹H NMR (DMSO-d₆, 200 MHz): d 1.13¾1.37 (m, 5 H, ring CH₂), 1.55¾1.60 (m, 1 H, ring CH₂), 1.72¾1.75 (m, 2 H, ring CH₂), 1.99¾2.03 (m, 2 H, ring CH₂), 3.02 ¾ 3.08 (m, 1 H, ring CH), 3.59 (dd, J = 6.2, 12.6 Hz, 1 H, CH_aH_bNH₂ ⁺), 3.82 (dd, J = 8.1, 12.1 Hz, 1H CH_aH_bNH₂ ⁺), 4.62 (t, J = 7.2, 1 H PhCHSSO₃^-), 7.36 (s, 5 H, aromatic CH), 8.51 (broad, 2 H, NH₂⁺); ¹³C NMR (DMSO-d₆, 50 MHZ): d 138.4 (C_ipso), 128.9 (o-CH), 128.0 (p-CH), 127.6 (m-CH), 56.8 (CH-1´), 49.6 (CH₂-2), 49.0 (CH-1), 28.5 [CH₂-2´(6´)], 24.7 (CH₂-4´), 23.8 [CH₂-3´(5´)]; MS (EI) m/z (%): 41 (41), 55 (33), 67 (23), 77 (43) [Ph]⁺, 86 (87), 91 (98) [C₇H₇]⁺, 103 (53), 104 (63), 118 (42), 120 (69), 121 (62), 135 (66), 146 (55), 162 (35), 175 (53), 176 (100) [PhCHCH₂NHC₄H₉] ⁺, 177 (40), 208 (38), 209 (42), 250 (39), 280 (30), 307 (40), 309 (71); MS (CI) m/z (%) 100 (16), 112 (41), 120 (19), 137 (26), 179 (13), 201 (17), 202 (80), 203 (14), 230 (10), 231 (49), 232 (82), 233 (28), 234 (45), 235 (18), 236 (100) [PhCHSH(CH₂NHC₆H₁₁ ) + H]⁺, 237 (16), 274 (13), 278 (12), 292 (14), 333 (55), 334 (29), 335 (59), 336 (26), 363 (16), 398 (15), 469 (22) [{PhCH(CH₂NHC₆H₁₁)S} ₂ + H]⁺; C₁₄H₂₁NO₃S₂ (315.4): calcd. C 53.3%, H 6.67, N 4.44%, S 20.25%; found C 53.37%, H 6.91%, N 5.21%, S 20.25%.

CONCLUSION

Seven new aminoalkanethiosulfuric acids were synthesized which differed in the alkyl group bound to the amine. The structures of the intermediates and products were verified by IR, NMR and mass spectroscopy and elemental analyses. Contrary to results previously observed¹⁷, a rearrangement via the aziridinium ion does not occur in the last step of the synthesis and the final product bears the thiosulfate group bound to the benzilic carbon. This series of compounds has been submitted for biological testing for activity against schistosomiasis in mice, as well as antitumorigenic, anti-fungal and radioprotective activities. Preliminary results in the tests for activity against schistosomiasis were very promising. Future studies will include analogs bearing substituents on the aromatic ring.

ACKNOWLEDGEMENTS

This work was supported by funds furnished by the Conselho Nacional de Desenvolvimento Científico - CNPq and the Fundação de Amparo a Pesquisa do Estado de Minas Gerais - FAPEMIG. Professors D. Piló-Veloso and D. L. Nelson were recipients of research fellowships from the CNPq. This work constitutes part of the Doctoral Thesis of L. S. A. Moreira. NMR spectra were determined at LAREMAR, Departamento de Química, ICEx, UFMG. The mass spectra of the aminoalcohols were determined at the Departamento de Química, ICEx, UFMG and the mass spectra of the aminoalkanethiosulfuric acids at the Fundação Centro Tecnológico de Minas Gerais (CETEC-MG). We thank the Departamento de Química da Universidade Federal de São Carlos for the use of the ACD/CNMR program.

18. Unpublished results.

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Publication Dates

• Publication in this collection
  12 Dec 2006
• Date of issue
  Aug 2000

History

• Accepted
  25 Nov 1999
• Received
  18 Feb 1999