CASTOR BEANS QUALITY SUBJECTED TO DIFFERENT STORAGE TEMPERATURES AND PERIODS

ABSTRACT

The control of the temperature of the storage air have the ability to improve a better preservation of some types of agricultural products. However, the most effective temperature for storage as well as the duration of storage varies between products. Therefore, special attention should be concentrated on the storage temperature and its effects on the integrity and longevity of the produce. The objective of the present study was to evaluate the effects of storage temperature and storage period on the quality of castor beans. Castor seeds with a water content of approximately 6.1% (w.b.) were stored for 180 days at temperatures of 15, 25 and 35 °C. The quality of the seeds was evaluated every 45 days throughout the study period by measuring dry matter loss, electrical conductivity, color, and the free fatty acid and peroxide content of the crude oil extracted. Our results indicated that: a) higher storage temperature negatively affect the quality of the seeds and the extracted oil; b) the negative effect of temperature increase with longer storage period; c) the storage temperature of 15 °C least affect the quality of castor bean and the extracted oil.

KEYWORDS
armazenability; Ricinus communis L.; post-harvest

INTRODUCTION

Castor bean (Ricinus communis L.) has become a highly versatile and promising crop in recent decades in terms of its highly profitable agroenergy supply chain and capacity to generate employment opportunities directly and indirectly (Poletine et al., 2012; Goneli et al., 2016). These benefits are due to some unique characteristics of castor bean, including their adaptability to a wide range of environmental conditions across temperate and semi-arid regions, and its high oil content (approximately 45%), which is rich in ricinoleic acid (90%). These beneficial characteristics prompted the development of the Brazilian National Biodiesel Program and a broad range of industrial applications, because the raw material can be used for the production of several products, including lubricants, paints, varnishes, foam products, plastics materials, food products, and pharmaceuticals products (Campos & Santos, 2015; Mohamed & Mursy, 2015).

Therefore, considering the range of application of castor bean, research interest has intensified to identify the limitations of the production of castor beans and to increase the value of the harvested crop. In this context, research advancements have improved the production and maintenance of castor bean, which in turn determine the market value and commercial viability of this crop (Negedu et al., 2013; Silva et al., 2013; David et al., 2014; Santoso et al., 2015). With the storage of the product, in addition to its qualitative maintenance, a better time for commercialization can be achieved due to the capacity to storage the material and wait for its most attractive price (Hartmann Filho et al., 2016). However, in Brazil, given the difficult interaction between the field and the commercial sector, generally, the static capacity becomes insufficient to meet the production and, a series of problems appear, compromising both market range and commercialization value (Nascimento et al., 2016).

Storage characteristics of castor bean are of great importance because they directly affect the quality and market value of the beans. With respect to storage practices, storage temperature and storage period, which are the most important variables; storage temperature can affect product quality during the storage period, while the storage period can accentuate product deterioration (Zonta et al., 2014; Polat, 2015; Dias et al., 2016). In this context, product deterioration involves dry matter losses, changes in color, and poor quality of by-products such as crude oil, due, mainly, to the lipidic deterioration, wich have the ability to elevate some undesirable characteristics undesirable to the oil, like the acidity and peroxide content (Del Campo et al. 2014; Paraginski et al. 2015; Hartmann Filho et al., 2016).

Therefore, the present study aimed to evaluate the effects of storage temperature and storage period on the quality of castor bean.

MATERIAL AND METHODS

The study included crop processing, storage, and evaluation of the quality of the castor beans and its crude oil, and was conducted at the Laboratory of Physical Properties and Quality of Agricultural Produce at the Viçosa Federal University in the city of Viçosa, Minas Gerais, Brazil.

The Guarani castor bean variety was used in this study. The fruits were harvested manually from the middle part of the first plant bundle with approximately 11% (w.b.) water content. (average water content that the product was collected in the cultivation region, Várzea da Palma, MG). Following this, the fruits were mechanically broken, and the seeds were separated from the shell. For the processing, a machine for corn on cob was adapted to the culture of castor bean. In view of the processing made in a machine adapted to the culture in study, a manual selection of grains, with no type of defect, was made to do not generate results inconsistent to the reality.

After processing, the water contents of the beans were approximately 6.1% (w.b.). The beans were transferred to jute sacks and subjected to storage temperatures of 15, 25, and 35 °C (to have a wide product temperature range) for 180 days. The analyses of the variables were determined at the start of the experiment and subsequently at intervals of 45 days. The products were stored in B.O.D. incubators at the three temperatures under the same storage conditions. The mean values of relative humidity of environmental air were recorded during the storage period using two thermo-hygrometers, six times a day. The temperature of the grain mass remained constant or in thermal equilibrium with the storage temperatures throughout the experiment.

Castor beans quality was evaluated by analyzing dry matter loss, electrical conductivity, and color. The quality of crude oil was assessed by determining the free fatty acid and peroxide content. The water content of each treatment was determined via gravimetric analysis in an oven at 105 ± 3 °C, for 24 h, with two repetitions (Brasil, 2009).

The dry matter of castor beans during storage was evaluated by performing five test repetitions for each treatment. Each repetition was composed of approximately 100 g of product, which was placed in perforated and waterproof packages, that were sealed and transferred to jute sacks containing the remaining beans. The mass of each repetition and water content of the material was measured at the beginning and then at 45-day intervals. The dry matter loss was calculated using [eq. (1)].

(1) $$\text{DML=}\frac{\frac{{\text{m}}_{\left(\text{i}\right)}}{\left(1+{\text{U}}_{\text{i}}^{*}\right)}-\frac{{\text{m}}_{\left(\text{θ}\right)}}{\left(1+{\text{U}}_{\text{θ}}^{*}\right)}}{\frac{{\text{m}}_{\left(\text{i}\right)}}{\left(1+{\text{U}}_{\text{i}}^{*}\right)}}100$$

Where,

DML: dry matter loss, %;

m_(i): initial mass of bean samples, g.;

m_(θ): mass of the samples at time θ, g;

U_i^*: initial water content of bean samples, decimal d.b., and

U_θ^*: water content of the samples at time θ, decimal d.b.

For the evaluation of electrical conductivity, four subsamples of 50 seeds were used in each treatment. Seeds were weighed and then hydrated using 75 mL of distilled deionized water for 24 h at 25 °C in 200 mL plastic cups. After this period, the electrical conductivity of the solution was determined using a Digimed conductivity meter, model DM3. Each reading (measured in μS cm^-1) was divided by its respective mass, and the final result of the test was expressed in μS cm^-1 g^-1 (Vieira & Krzyzanowski, 1999).

The color was assessed by directly reading the reflectance of the beans using a tristimulus colorimeter (illuminant 10°/D65) and the Hunter color scale. Coordinate values were obtained for the coordinates “L” (luminosity), “a” (green and red hues), and “b” (blue and yellow hues). For each measurement, an average of three readings was obtained.

Using the values of the perceived variations represented in the coordinates “L”, “a”, and “b” (Equations 2, 3, and 4, respectively), we calculated the total color difference (Equation 5) and the chroma index, which defined color intensity and purity (Equation 6).

(2) $$\Delta {\text{L=L}}_{\left(\text{t}\right)}-{\text{L}}_{\left({\text{t}}_{\text{0}}\right)}$$

(3) $$\Delta {\text{a=a}}_{\left(\text{t}\right)}-{\text{a}}_{\left({\text{t}}_{\text{0}}\right)}$$

(4) $${\Delta }_{\text{b}}{\text{=b}}_{\left(\text{t}\right)}-{\text{b}}_{\left({\text{t}}_{\text{0}}\right)}$$

(5) $$\Delta \text{E=}\sqrt{\Delta {\text{L}}^{\text{2}}+\Delta {\text{a}}^{\text{2}}+\Delta {\text{b}}^{\text{2}}}$$

(6) $${\text{C}}_{\text{r}}\text{=}\sqrt{{\text{a}}^{\text{2}}+{\text{b}}^{\text{2}}}$$

Where,

ΔE: color difference;

C_r: chroma index;

t: storage period, days, and

t₀: beginning of the storage period.

The crude oil was extracted according to the norms of the AOCS (1993), method Ac 3-44. A Soxhlet extractor was used with hexane solvent. Extraction was performed for six hours.

The free fatty acid content was measured in accordance with the AOCS (2012), method Ca 5a-40, by dissolving three samples 5 g oil samples in ethyl alcohol, heating the solution to 60 - 65 °C, and titration with 0.1 N sodium hydroxide 0.1 N. The fatty acid content was calculated using [eq. (7)].

(7) $$\text{FFA=}\frac{{\text{CV}}_{\text{g}}\text{N}}{\text{m}}$$

Where,

FFA: free fatty acids, %;

C: molar mass of the predominant acid;

V_g: volume of standardized NaOH solution, ml;

N: normality of NaOH solution, and

m: sample mass, g.

The predominant fatty acid in castor beans is ricinoleic acid, with a molar mass of 298 g mol^-1.

The peroxide content was determined in accordance with the AOCS (2011), method Cd 8b-90, by dissolving three 5 g oil samples in 50 mL of a solution of acetic acid isooctane (3:2, v/v), with addition of 0.5 mL of a saturated solution of potassium iodide, followed by trituration with 0.01 N sodium thiosulfate solution (Na₂S₂O₃) 0.01 N. The volume used in titration after addition of 0.5 mL of the starch indicator solution indicated the peroxide concentration in meq of peroxide kg^-1, using [eq. (8)]:

(8) $$\text{PI=}\frac{\left[\text{N}\left(\text{A}-\text{B}\right)1000\right]}{\text{m}}$$

Where,

PI: peroxide index content in meq kg^-1 of the lipid fraction;

A: Volume of the sodium thiosulfate (Na₂S₂O₃) solution used during trituration, in ml;

B: Volume of the sodium thiosulfate (Na₂S₂O₃) solution used during titration of the reagents without the sample, in ml;

N: normality of the sodium thiosulfate (Na₂S₂O₃) solution, and

m: sample mass, in g.

The experiment was conducted in subdivided plots 3 x 5, with three storage temperatures in each plot and five distinct storage periods in each subplot, in an entirely random design, with four replicates. The latent effect was evaluated by subjecting the data to polynomial regression analysis. The models were selected on the basis of the size of the coefficient of determination (R²), the significance of regression (using the F test), and the biological phenomenon under study.

RESULTS AND DISCUSSION

The water content of the castor beans throughout the storage period varied during the study. However, this may be a result of the hygroscopic characteristics of the beans and relative humidity variation during the storage. Consequently, phenomena such as sorption and desorption likely occured, as described by Tiecker Junior et al. (2014) and Bessa et al. (2015) (Table 1).

TABLE 1
Average water content (% w.b.) of beans during storage at different temperatures.

Storage period (days)	Temperature (°C)
	15	25	35
0	6.1	6.1	6.1
45	8.2	6.7	4.5
90	8.2	6.4	4.9
135	7.4	6.4	4.9
180	7.8	6.3	4.9

Moreover, throughout the 180 day storage period, the increase in the storage temperature decreased the equilibrium water content of the beans; this occurred because relative humidity decreased as the storage temperature increased and resulted in a lower equilibrium water content (Table 1). The average relative humidity at the storage temperatures of 15, 25 and 35 °C, was 75%, 62%, and 40%, respectively.

It is worth mentioning that although all treatments presented the same initial water content (at t₀), the treatments at the lowest and highest temperatures (15 and 35 °C) presented a greater variation between the initial and final values. This indicates that, because the temperature directly affected ambient relative humidity, the water content of the beans became susceptible to alterations in either sorption (in the case of beans stored at 15 °C) or desorption (in the case of beans stored at 35 °C (Table 1).

The evaluation of the dry matter loss during storage revealed that, in general as storage temperature increased, dry matter loss increased. This effect was accentuated as storage period increased (Figure 1). After 45 days of the storage period - the first evaluation phase - the dry matter loss observed for storage temperatures of 15, 25, and 35 °C was 0.17%, 0.22%, and 0.29%, respectively (Figure 1). Therefore, due the storage time potentiated the deleterious effects, as evidenced by the multiplicative parameters of the adjusted equations and by the linear manner as the storage progressed, at 180 days storage was observed losses of 0.7%, 0.9%, and 1.2%, respectively.

FIGURE 1
Dry matter loss in castor beans, as a function of storage temperature and storage period.

Therefore, independent of the storage temperature used, the dry matter loss exceeded 0.5%. However, this loss occurred at different periods at the three temperatures evaluated, and corresponded to 135, 100, and 77 days storage at 15, 25, and 35 °C, respectively (Figure 1). Del Campo et al. (2014) and Paraginski et al. (2015) found that storage temperature directly affected dry matter loss, due the capacity of this factor influence the respiratory processes, increasing, or not, the metabolic rate of the product, and therefore the consumption of its reserves. Therefore, while the process of deterioration is inevitable, this process can be attenuated by using lower storage temperatures to increase product storage (Coradi et al., 2015a).

The analysis of electrical conductivity indicated that the increase in the storage temperature was a determining factor in the decrease of bean quality, as well as storage time. This change in electrical conductivity was observed, generally, at 45 days, due to the increased in the results with the elevation of temperature, and progressed linearly over time in this action (Figure 2). Furthermore it was observed for the adjusted equations the elevation of multiplicative coefficients as the elevation of the storage temperature, which proved the negative effect of the increase factor over time.

FIGURE 2
Electrical conductivity of absorption solution of castor beans as a function of storage temperature and storage period.

Our study design also provided information on the integrity of the seeds - in particular on its cellular membranes. An increase in storage temperature and of storage period accentuated the deterioration processes, causing physical and structural damage in the beans. This damage might have contributed to lixiviation of the solubles, which are essential for the preservation of the quality of the produce and, consequently, its storability. With the deterioration of cellular integrity, lixiviation of solubles occurs because the cellular membranes no longer efficiently function as a selective barrier at the start of absorption, and thus resulted in higher electrical conductivity values in the solution (Dias et al., 2016; Hartmann Filho et al., 2016). Deterioration often occurs when the product is stored under extreme conditions, such as storage for long periods at high temperatures because membrane integrity is gradually deteriored (Bezerra et al., 2015).

Bean color during storage also underwent changes, which were also accentuated at higher storage temperatures (Figure 3). Change in color was revealed by the darkening of the beans, which was evidenced by the decrease in the “L,” “a,” and “b” values (Figure 4). Therefore, darkening occurred when the produce exhibited values corresponding to darker hues, such as black rather than white (Figure 4a), green rather than red (Figure 4b), and blue rather than yellow (Figure 4c).

FIGURE 3
Differences in bean color as a function of storage temperature and storage period.

FIGURE 4
Differences in the coordinates “L” (a), “a” (b), and “b” (c) of castor beans, as a function of storage temperature and storage period.

Similar results were observed by Hartmann Filho et al. (2016) when evaluating the color of coffee beans after harvest. The authors observed the darkening of the produce, which was expressed as a decrease in the values of “L,” “a,” and “b,” and this darkening correlated with higher storage temperatures and longer storage periods.

It is likely that higher storage temperatures increased the metabolic rate of the produce, as observed in Figure 3 for dry matter loss, and directly affected the product's appearance. So, this variable was demonstrated by the fact that the increase in storage temperature affected bean quality, and the effect was accentuated by the increase in the storage period. This result corroborates the findings of Coradi et al. (2015b) for soybeans and of Elias et al. (2016) for kidney beans.

In addition, the change in coloration (darkening) could be confirmed by determining the chroma index. This index reveals the extent of reduction in the saturation of the typical colors of the material, which acquires a more grayish hue, as observed by Mcguire (1992) (Figure 5). Silochi et al. (2016) reported that the chroma index could be used to characterize product quality, which directly impacts market acceptance and commercialization.

FIGURE 5
Chroma index of castor beans as a function of the storage temperature and storage period.

The analysis of the extracted oil indicated that higher storage temperatures were correlated with increased free fatty acid and peroxide content, beginning at the first observation period of 45 days storage. The differences in contents were higher between storage temperatures of 15 and 35 °C, and smaller between storage temperatures of 15 and 25 °C (Figure 6). The free fatty acid content of crude oil extracted from beans stored at 15, 25, and 35 °C was 0.27%, 0.29%, and 0.36% at 45 days of storage (Figure 6a), whereas the peroxide content under the same conditions was 2.21, 2.44, and 4.56 meq kg^-1 of the lipid fraction (Figure 6b).

FIGURE 6
Free fatty acid content (a) and peroxide content (b) of crude oil extracted from castor beans as a function of storage temperature and storage period.

However, due to product deterioration during storage, which was evident by an increase in the multiplicative coefficients of the adjusted equations, the harmful effect of higher temperatures may be accentuated. This effect was corroborated by the results observed at 90 days of storage, when the free fatty acid content at 15, 25, and 35 °C was 0.33%, 0.39%, and 0.57%, respectively, and peroxide content at these temperatures was 3.07, 3.68, and 6.35 meq kg^-1 of the lipid fraction (Figure 6).

Bezerra et al. (2015) observed that the quality of castor bean crude oil was influenced by ambient conditions and the storage period of the raw material. The degree of preservation of the raw material can determine the properties of the by-products. In addition, the quality of the crude oil can be used as a parameter for evaluating the degree of preservation of the raw material because characteristics such as the free fatty acid content indicates the stage of deterioration of the raw material (Hartmann Filho et al., 2016).

Despite the depreciation of the castor bean crude oil with storage temperature and period, we found that 180 days storage of castor beans resulted in qualities that were lower than the limits established by ANVISA (Brasil, 2005). Our results indicated that the acidity index of the crude oil from castor beans at 15, 25, and 35 °C at 180-days storage was 0.90, 1.17, and 1.96 mg KOH g^-1, respectively, and the peroxide content for the same treatments was 4.78, 6.15, and 9.92 meq kg^-1 of the lipid fraction (see Figure 6). This restricts the commercialization of castor bean crude oils in Brazil since acidity indexes exceed 4.0 mg KOH g^-1 and peroxide content exceeds 15.0 meq kg^-1 of the lipid fraction.

The acidity of castor bean oil can also be determined using the acidity index, which is defined as the amount of KOH (in mg) necessary to neutralize 1 gram of sample. The free fatty acid content can be converted to the acidity index by multiplying the value by 1.99. Crude castor bean oil can be subclassified using this index. According to Santos et al. (2007) reported that this oil could be commercially classified as medicinal (no acidity), industrial n^o 1 (maximum acidity of 1%), and industrial n^o 3 (maximum acidity of 3%). Therefore, castor bean oil can be classified as industrial oil n^o 1 when the beans were stored at 15 °C for 180 days and industrial n^o 3 when stored at 25 or 35 °C for 180 days.

CONCLUSIONS

Increased storage temperature negatively affect the quality of castor beans and its byproducts. This effect is accentuate as the storage period increased from 15 to 35 °C and storage duration increase from 45 days to 180 days.

The highest grade commercial quality oil is only achieve when beans were stored at 15 °C for up to 180 days.

ACKNOWLEDGEMENTS

To the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and the Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (Fundect) due the financial support for research

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Publication Dates

• Publication in this collection
  May-Jun 2018

History

• Received
  07 Apr 2017
• Accepted
  16 Oct 2017