Evaluation of electromyographic activity and heart rate responses to isometric exercise. The role played by muscular mass and type

Braz J Med Biol Res, January 1999, Volume 32(1) 115-120 (Short Communication)

Evaluation of electromyographic activity and heart rate responses to isometric exercise. The role played by muscular mass and type

E. Silva^1,4, L. Oliveira¹, A.M. Catai¹, P. Ferreira-Filho², F. Bérzin³ and L. Gallo Jr.⁵

¹Laboratório de Fisioterapia Cardiovascular, Departamento de Fisioterapia, and ²Departamento de Estatística, Universidade Federal de São Carlos, São Carlos, SP, Brasil

³Departamento de Morfologia, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba, SP, Brasil

⁴Departamento de Fisiologia e Biofísica, Universidade Estadual de Campinas, Campinas, SP, Brasil

⁵Divisão de Cardiologia, Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil

^Text

Abstract

References

^{Correspondence and Footnotes}

The purpose of the present study was to examine the relationship between the electromyographic (EMG) activity and heart rate (HR) responses induced by isometric exercise performed by knee extension (KE) and flexion (KF) in men. Fifteen healthy male subjects, 21 ± 1.3 years (mean ± SD), were submitted to KE and KF isometric exercise tests at 100% of maximal voluntary contraction (MVC). The exercises were performed with one leg (right or left) and with two legs simultaneously, for 10 s in the sitting position with the hip and knee flexed at 90^o. EMG activity (root mean square values) and HR (beats/min) were recorded simultaneously both at rest and throughout the sustained contraction. The HR responses to isometric exercise in KE and KF were similar when performed with one and two legs. However, the HR increase was always significantly higher in KE than KF (P<0.05), whereas the EMG activity was higher in KE than in KF (P<0.05), regardless of the muscle mass (one or two legs) involved in the effort. The correlation coefficients between HR response and the EMG activity during KE (r = 0.33, P>0.05) and KF (r = 0.15, P>0.05) contractions were not significant. These results suggest that the predominant mechanism responsible for the larger increase in HR response to KE as compared to KF in our study could be dependent on qualitative and quantitative differences in the fiber type composition found in each muscle group. This mechanism seems to demand a higher activation of motor units with a corresponding increase in central command to the cardiovascular centers that modulate HR control.

Key words: knee, isometric exercise, heart rate, electromyography, rectus femoris, biceps femoris

The autonomic efferent mechanisms responsible for the tachycardia induced by isometric exercise are well known. Both vagal withdrawal and a sympathetic stimulation of the sinus node may contribute in different degrees to the exercise tachycardia, depending on the duration and intensity of isometric effort (1,2). However, studies on cardiovascular responses related to isometric exercise involving muscles of different group types show conflicting results. There are reports showing that the increase in heart rate (HR) during isometric exercise is determined by the duration, percentage of maximal voluntary contraction (%MVC), muscle length, and the amount and size of active muscle mass (3-5). In contrast, some investigators (6,7) have reported that HR response to isometric exercise is related to the intensity of the maximum tension achieved rather than to the amount or size of the mass of the contracting muscle group. Also, it is worth mentioning the findings of other investigators showing that the HR increase is independent of muscle length and the external torque under conditions of controlled relative intensity of the MVC or muscle mass (8). In this context, it should be taken into consideration that muscle length is modified by the joint angle, and the maximal internal muscle force is directly related to muscle length (9). The purpose of the present study was to examine the relationship between the electromyographic (EMG) activity and HR response to isometric exercise in men, performed by a small (one leg) and large (two legs) muscle mass of different muscle groups at the same joint angle, with the same intensity (100% MVC), for 10 s.

Fifteen healthy male subjects were studied. Their anthropometric characteristics reported as mean and standard deviations were: age, 21 ± 1.3 years, weight, 81 ± 6.2 kg, and height, 182 ± 3.9 cm. All subjects were submitted to a medical examination prior to the study, including a resting electrocardiogram (ECG). They were informed about the study design and its objectives, and each subject signed a formal consent form, as required by the Ethics Committee of the Universidade Estadual de Campinas. The experimental protocol consisted of isometric exercises of knee extension (KE) and flexion (KF) at 100% MVC. The exercises were performed using a special chair (10), with the subject in a seated position, with hip and knees in flexion at 90^o. The subjects were stabilized in the chair with Velcro straps placed on the chest, pelvis and midthigh, to minimize the contraction of other muscles not directly involved in the exercise test. The resistance pad at the end of the lever arm was strapped approximately 2.5 cm above the medial malleolus. The subjects were instructed to breathe spontaneously and not to perform a Valsalva maneuver while straining, to avoid reflex HR responses due to cardiovascular changes. They performed six tests of isometric exercises in KE and KF, with one (left and right leg in random order) and with both legs at 100% MVC for 10 s. The subjects were verbally instructed by the investigators to use their maximal force and were vigorously encouraged to maintain it at a constant level for a period of 10 s, "Are you ready? Now push! Push harder! Harder! And relax". The interval between two successive tests was set at 10 min when HR values had always returned to basal conditions. The HR was obtained from an ECG. The signals were recorded in real time after analog to digital conversion (ADC), and the identification of "R" peaks of the ECG waves; the R-R intervals were calculated on a beat-to-beat basis using a special software developed in our laboratory (11). Recordings were obtained for 65 s before each exercise, throughout the sustained contraction, and for 285 s during the recovery period. The resting HR values (beats/min) correspond to an average obtained during a period of 65 s immediately before the beginning of exercise and the peak HR response was expressed as the highest instantaneous value attained at 10 s of exercise (DHR = 0-10 s). The HR response corresponding to the effort of the right or left leg during the isometric exercise with only one leg was expressed as the mean value of the two legs.

EMG activity was recorded using a multichannel electromyographic system (Nicolet Viking II) with a self-adhesive Beckman surface mini-bipolar electrode, consisting of two silver/silver chloride electrodes and a solid-state preamplifier in a plastic enclosure, applied to the skin over the following muscle groups: 1) right and left rectus femoris; 2) right and left biceps femoris; 3) right and left intercostal muscles between the 5th and 6th intercostal spaces, and left pectoralis major. The rectus femoris and biceps femoris muscle groups were mainly involved in knee extension and flexion, respectively.

EMG signals were also recorded in real time after ADC, sampled at a frequency of 250 Hz, with band filters having a low cut-off frequency and a high cut-off frequency set at 10 Hz and 10 kHz, respectively. Using the MMP software (Multi Mode Program) the data were processed and presented as graphic and numerical values. The EMG data included the 10 s of muscular effort (100% MVC) as well as 5 s before and after each test. The magnitude of the EMG signal in microvolts (µV) was presented as the root mean square values (RMS) for the purposes of quantifying the electromyographic activity during each isometric contraction. The EMG signal corresponding to the effort of the right or left leg during the isometric exercises with only one limb is reported as the mean value of the two legs.

The results are presented in a box-plot as median, 1st and 3rdquartiles, range and outliers. Statistical analysis was conducted using the Friedman nonparametric multiple comparison tests. The relationship between the EMG and HR responses to exercise was reported using Spearman rank correlation coefficients (12). The differences were considered significant at the P<0.05 level. Analysis was performed using the software package STATISTICA for Windows, Release 5.1. StatSoft, Inc. 1994-1996 and BMDP 3S Nonparametric Statistics software package.

HR values at rest were always similar immediately before the knee extension and flexion isometric tests. The HR increased significantly above the control levels under all exercise conditions (P<0.05). Peak HR (DHR = 0-10 s) attained at the end of the isometric exercise was significantly higher in KE than KF (P<0.05; Figure 1). However, the magnitude of the HR increment induced by isometric exercise for each modality of contraction (KE and KF) was similar when exercise was performed with one and two legs (P>0.05, Figure 1).

The EMG signal (µV) values (RMS) during each trial of sustained contractions were always higher in KE than KF, independently of whether contraction was performed with one or two legs (P<0.05; Figure 2). The correlation between the RMS values of the EMG signal of the contracting muscles and the HR responses was not statistically significant for KE (r = 0.33, P>0.05) or KF (r = 0.15, P>0.05).

Figure 1
- Heart rate (DHR - beats/min) responses at 10 s of maximal isometric exercise tests. (A) and (C) report the values for the unilateral right and left extension and flexion exercises, and (B) and (D) report the values for the bilateral knee extension and flexion exercise. Data are reported as medians, 1st, and 3rd quartiles and range.

Figure 2
- Amplitude of electromyographic signals are reported as root mean square values (RMS) (µV) at 10 s of maximal isometric exercise tests, with knee extension and flexion. (A) and (C) report the values for the unilateral right and left extension and flexion exercises and (B) and (D) report the RMS values, as a sum of both knees, from bilateral extension and flexion exercises. Data are reported as medians, 1st and 3rd quartiles and range.

Considering the time course and the magnitude of the HR response to maximal isometric exercise at 10 s, our results under all conditions were similar to those reported in the literature (1,2). However, our results show that the quantity of muscle mass involved did not appear to have a significant influence on the HR response under the conditions studied, i.e., maximal isometric contraction with one or two legs (approximately twice the quantity of muscle mass), involving extension or flexion of the knee, with hip and knee positioned at 90^o flexion. Nevertheless, we observed that the magnitude of the HR response was significantly greater in the extension than in the flexion isometric exercise.

The EMG activity and HR responses to the MVC effort found in the present study are in agreement with recent observations that the magnitude of these variables is influenced by the types of muscles participating in the process of muscle contraction (4-6). In this context, our data support the idea that the different HR responses are related to the activation of the motor units of the muscle groups involved in the contraction process. In relation to these findings, it should be emphasized that the magnitude of the EMG and HR responses could be significantly different as a function of the structural and functional characteristics of a particular muscle fiber found in each muscle group during contraction (4,13), depending on its slow (type I, red, tonic, and aerobic fibers) or fast (type II, white, and anaerobic fibers) nature. It has been found that slow contraction fibers compared to fast contraction fibers present: 1) a low threshold for recruitment of the motor neurons, 2) higher firing rates, 3) a high energetic efficiency and resistance to fatigue, and 4) a lower ratio between frequency and depolarization during the production of force (13). Also, it has been noted that during force generation the motor units with a high threshold for recruitment begin to fire at higher rates than the lower ones, which could cause the occurrence of an early fatigue and as a consequence a greater recruitment of motor units to maintain the initial force (9,13). Another aspect to be considered is that the relative quantity of fast and slow muscle fibers in humans differs in size and number for each specific muscle group and even in the same group depending on their functional specificity. Thus, these findings are in accordance with our present study, since the main muscles involved in knee extension and flexion, the rectus femoris and the biceps femoris, also have a higher percentage of fast and slow twitch fibers, respectively (14,15).

These results suggest that the additional recruitment of motor units for the development and maintenance of the maximal voluntary effort of extension, with the right and/or left leg, could be attributed to the differences in the percentages of fiber types found in muscles involved in the experimental conditions studied. As a consequence of these arguments, the increase in the recruitment of the motor fibers involved in knee extension should demand greater activation of central command (3,4).

It is possible that the lack of correlation between our EMG and HR responses could be due to a non-linear relationship between these two variables. Under these circumstances it is plausible to propose that the explanation for the absence of an increase in magnitude of the HR response in two-legs versus one-leg conditions might be due to saturation of central irradiation over cardiovascular bulbar centers. Finally, we should take into consideration that the differences between our results and those of other studies (7,8) concerning the relationship of EMG signal and HR may also be related to diversity of experimental protocols, since most of them were conducted on muscle groups of different type and size, at levels usually below 100% MVC and with longer time contraction.

In conclusion, our data support the idea that under conditions of maximum isometric effort the main modulating mechanism of the HR response is related to the specificity of the central command for the muscle groups with their corresponding pattern of fiber recruitment.

1. Martin CE, Shaver JA, Leon DF, Thompson ME, Reddy PS & Leonard JJ (1974). Autonomic mechanisms in hemodynamic responses to isometric exercise. Journal of Clinical Investigation, 54: 104-115.

2. Maciel BC, Gallo Jr L, Marin-Neto JA & Martins LEB (1987). Autonomic nervous control of the heart rate during isometric exercise in normal man. Pflügers Archives, European Journal of Physiology, 408: 173-177.

3. Ramos UM, Mundale MO, Awad EA, Witsoe DA, Cole TM, Olson M & Kottke FJ (1973). Cardiovascular effects of spread of excitation during prolonged isometric exercise. Archives of Physical Medicine and Rehabilitation, 54: 496-504.

4. Mitchell JH, Pyne HR, Saltin B & Schibye B (1980). The role of muscle mass in the cardiovascular response to static contractions. Journal of Physiology, 309: 45-54.

5. Seals DR, Washburn RA, Hanson PG, Painter PL & Nagle FJ (1983). Increased cardiovascular response to static contraction of larger muscle groups. Journal of Applied Physiology: Respiratory Environmental and Exercise Physiology, 54: 434-437.

6. McCloskey DI & Streatfeild KA (1975). Muscular reflex stimuli to the cardiovascular system during isometric contractions of muscle groups of different mass. Journal of Physiology, 250: 431-441.

7. Williams CA (1991). Effect of muscle mass on the pressor response in man during isometric contractions. Journal of Physiology, 435: 573-584.

8. NG AV, Agre JC, Hanson P, Harrington MS & Nagle FJ (1994). Influence of muscle length and force on endurance and pressor responses to isometric exercise. Journal of Applied Physiology, 76: 2561-2569.

9. Basmajian JV & De Luca CJ (1985). Muscle Alive, Their Functions Revealed by Electromyography. 5th edn. Williams & Wilkins, Baltimore.

10. Monteiro-Pedro V (1995). Atividade eletromiográfica do músculo vasto medial oblíquo em esforços isométricos e isotônicos das articulações do quadril e do joelho. Doctoral thesis, Faculdade de Odontologia de Piracicaba, UNICAMP, Piracicaba.

11. Silva E, Catai AM, Trevelin LC, Guimarães JO, Silva Jr LP, Silva LMP, Oliveira L, Milan LA, Martins LEB & Gallo Jr L (1994). Design of computerized system to evaluate the cardiac function during dynamic exercise. Physics in Medicine and Biology, 33: 409 (Abstract).

12. Siegel S (1956). Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, Tokyo.

13. Erim Z, De Luca CJ, Mineo K & Aoki T (1996). Rank-ordered regulation of motor units. Muscle and Nerve, 19: 563-573.

14. Polgar J, Johnson MA, Weigtman D & Appleton D (1973). Data on fibre size in thirty-six human muscles - an autopsy study. Journal of Neurological Sciences, 19: 307-318.

15. Johnson MA, Weightman D & Applenton D (1973). Data on the distribution of fibre types in thirty-six human muscles. Journal of Neurological Sciences, 18: 111-129.

The authors are deeply grateful to Vera Regina Fernandes da Silva Marães and Carlos Augusto Lemos Mega for technical assistance.

Address for correspondence: E. Silva, Laboratório de Fisioterapia Cardiovascular, Departamento de Fisioterapia, UFSCar, Via Washington Luiz, km 235, 13565-905 São Carlos, SP, Brasil. Fax: [phone]. E-mail: [e-mail]

Research supported by CNPq (No. 520.686/95-0). Publication supported by FAPESP. Received July 20, 1998. Accepted October 28, 1998.

Acknowledgments

References

• 1. Martin CE, Shaver JA, Leon DF, Thompson ME, Reddy PS & Leonard JJ (1974). Autonomic mechanisms in hemodynamic responses to isometric exercise. Journal of Clinical Investigation, 54: 104-115.
• 2. Maciel BC, Gallo Jr L, Marin-Neto JA & Martins LEB (1987). Autonomic nervous control of the heart rate during isometric exercise in normal man. Pflügers Archives, European Journal of Physiology, 408: 173-177.
• 3. Ramos UM, Mundale MO, Awad EA, Witsoe DA, Cole TM, Olson M & Kottke FJ (1973). Cardiovascular effects of spread of excitation during prolonged isometric exercise. Archives of Physical Medicine and Rehabilitation, 54: 496-504.
• 4. Mitchell JH, Pyne HR, Saltin B & Schibye B (1980). The role of muscle mass in the cardiovascular response to static contractions. Journal of Physiology, 309: 45-54.
• 5. Seals DR, Washburn RA, Hanson PG, Painter PL & Nagle FJ (1983). Increased cardiovascular response to static contraction of larger muscle groups. Journal of Applied Physiology: Respiratory Environmental and Exercise Physiology, 54: 434-437.
• 6. McCloskey DI & Streatfeild KA (1975). Muscular reflex stimuli to the cardiovascular system during isometric contractions of muscle groups of different mass. Journal of Physiology, 250: 431-441.
• 7. Williams CA (1991). Effect of muscle mass on the pressor response in man during isometric contractions. Journal of Physiology, 435: 573-584.
• 8. NG AV, Agre JC, Hanson P, Harrington MS & Nagle FJ (1994). Influence of muscle length and force on endurance and pressor responses to isometric exercise. Journal of Applied Physiology, 76: 2561-2569.
• 10. Monteiro-Pedro V (1995). Atividade eletromiográfica do músculo vasto medial oblíquo em esforços isométricos e isotônicos das articulaçőes do quadril e do joelho. Doctoral thesis, Faculdade de Odontologia de Piracicaba, UNICAMP, Piracicaba.
• 11. Silva E, Catai AM, Trevelin LC, Guimarăes JO, Silva Jr LP, Silva LMP, Oliveira L, Milan LA, Martins LEB & Gallo Jr L (1994). Design of computerized system to evaluate the cardiac function during dynamic exercise. Physics in Medicine and Biology, 33: 409 (Abstract).
• 12. Siegel S (1956). Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, Tokyo.
• 13. Erim Z, De Luca CJ, Mineo K & Aoki T (1996). Rank-ordered regulation of motor units. Muscle and Nerve, 19: 563-573.
• 14. Polgar J, Johnson MA, Weigtman D & Appleton D (1973). Data on fibre size in thirty-six human muscles - an autopsy study. Journal of Neurological Sciences, 19: 307-318.
• 15. Johnson MA, Weightman D & Applenton D (1973). Data on the distribution of fibre types in thirty-six human muscles. Journal of Neurological Sciences, 18: 111-129.

Correspondence and Footnotes

Publication Dates

• Publication in this collection
  06 Jan 1999
• Date of issue
  Jan 1999

History

• Accepted
  28 Oct 1998
• Received
  20 July 1998