TESES E DISSERTAÇÕES/THESIS AND DISSERTATIONS
TITLE: Regeneração de calos de arroz selecionados para resistência ao NaCl
AUTHOR: Kátia Helena Lipp João
DATE: April 15, 1987
PLACE: Botany - UFRGS - Porto Alegre
LEVEL: Master
EXAMINER BOARD:
José A. Peters (Co-adviser)
Alfredo G. Ferreira (Adviser)
Regina R. Termignoni - UFRGS
Maria T. Schifino Wittmann - UFRGS
Gilberto B. Kerbauy - USP
Eliane A. Oliveira - EMBRAPA
ABSTRACT - High calli percentage were induced from mature embryos of BR-IRGA 410, BR-IRGA 411 and Bluebelle rice cultivars. The basic culture medium, MS-62, was supplemented with three combinations of growth regulators: (1) 1mg/1 2,4-D + 0.2mg/l K + 0.2mg/l AIA; (2) 0.5mg/l 2,4-D + 0.2mg/l K + 0.2mg/l AIA; (3) 1mg/12,4-D + 0.2mg/l K. Bluebelle had the lowest calli induction rate. The medium 3 was the most efficient for calli induction and growth in all the cultivars. The calli were subcultured into the same media supplemented with 0, 0.5, 1.0 and 1.5% of NaCl for two passages (about 60 days). High survival rates were obtained and transferred to four shoot regeneration media (2mg/l K + 0.2 AIA; 3mg/l K + 0.2 AIA; 4mg/l K + 0.2 AIA; and 150mg/l glutamine + 0.02 K). The best regeneration media contained 2 or 3mg/l kinetine. The resulted shoots were transferred to a rooting medium (MS-62 + 0.2mg/l AIA). From a total of 2,072 inoculated calli, 200 planteets were regenerated and 166 completed their life cycles. Morphologic variations (extralong grains and awns) were observed for 77% of Bluebelle regenerated plants. Variations in acid phosphatase and esterase isozymes were detected for 91% of regenerates from all cultivars. The morphological and isozymic variations, as well as the possible calli salinity resistance were probably induced through somaclonal variations.
Publication Dates
-
Publication in this collection
17 June 2011 -
Date of issue
Dec 1988
