Abstracts
We provide notes on the design and construction of an Electric Fish Finder - a portable differential amplifier for detecting the electrostatic fields of gymnotiform knifefishes. The device can be adapted to locate electric fishes in any kind of aquatic environment. It is rugged, water resistant, and powered by alkaline batteries. This contribution is part of a series of technical papers designed to encourage students of neotropical ichthyology to explore the ecology, systematics, and electric signaling of gymnotiform fishes.
Electric Organ Discharge; Gymnotiformes; Knifefishes; Method
Nós fornecemos o esquema e as instruções para a construção de um Detector de Peixes Elétricos - um amplificador portátil de banda larga utilizado para detectar os campos elétricos de peixes gymnotiformes. Este aparelho pode ser adaptado para encontrar peixes elétricos em diversos ambientes aquáticos. É resistente, à prova d'água e energizado por baterias alcalinas. Esta contribuição faz parte de uma série de artigos técnicos que pretende encorajar estudantes de ictiologia neotropical a explorar a ecologia, sistemática e comunicação elétrica de peixes gymnotiformes.
SCIENTIFIC NOTE
Design and construction of an Electric Fish Finder
William G. R. CramptonI; Jonathan K. WellsII; Charles SmythII; Steven A. WalzI
IUniversity of Central Florida, Department of Biology, 4000 Central Florida Blvd., Orlando, FL, 32816-2368
IIUniversity of Florida, Department of Electrical and Computer Engineering, PO Box 116200, Gainesville, FL, 32611
ABSTRACT
We provide notes on the design and construction of an Electric Fish Finder - a portable differential amplifier for detecting the electrostatic fields of gymnotiform knifefishes. The device can be adapted to locate electric fishes in any kind of aquatic environment. It is rugged, water resistant, and powered by alkaline batteries. This contribution is part of a series of technical papers designed to encourage students of neotropical ichthyology to explore the ecology, systematics, and electric signaling of gymnotiform fishes.
Key words: Electric Organ Discharge, Gymnotiformes, Knifefishes, Method.
RESUMO
Nós fornecemos o esquema e as instruções para a construção de um Detector de Peixes Elétricos - um amplificador portátil de banda larga utilizado para detectar os campos elétricos de peixes gymnotiformes. Este aparelho pode ser adaptado para encontrar peixes elétricos em diversos ambientes aquáticos. É resistente, à prova d'água e energizado por baterias alcalinas. Esta contribuição faz parte de uma série de artigos técnicos que pretende encorajar estudantes de ictiologia neotropical a explorar a ecologia, sistemática e comunicação elétrica de peixes gymnotiformes.
Introduction
Wells & Crampton (2006) provide a detailed description of the design, construction and calibration of a bio-amplifier for research on electric fishes. This device was designed primarily for laboratory investigations of the repetition rate and waveform structure of gymnotiform electric organ discharges (EODs). Here we provide notes on the design and construction of a simpler and more rugged device intended to function as an 'Electric Fish Finder' (EFF) in the field. The EFF amplifies electric fish signals from a submerged dipole electrode and converts them into sound via an integrated loud speaker. The EFF is an indispensable tool for the rapid localization and targeted capture of electric fishes, especially in habitats where they are relatively uncommon.
Specifications. The EFF described here is a differential amplifier designed to accept signals from a submerged, grounded dipole electrode via a differential input. Amplification is controlled by a three position switch: off; low-gain (x 100); and high-gain (x 500). For either low or high-gain, the signal is further amplified (x 20) and converted into sound via an integrated loud speaker.
Power is provided by two 9V alkaline (or Ni-Mh rechargeable) batteries, allowing the unit to operate continuously for up to 24 hours at the low-gain setting, and 14 hours at high-gain. The unit weighs 0.55 kg (0.63 kg with batteries). The circuit board and battery bay are contained within a watertight aluminum enclosure, and the loud speaker, dipole connection, and switch are all also sealed so that the unit is resistant to dust, splashes, and short periods of total immersion in water. The unit can also survive a drop of up to 1 m onto a hard surface with no damage to the components. Swivels allow the attachment of a carrying strap so that the unit can be hung around the neck.
Field tests demonstrated that the EFF picks up clear signals from gymnotiform fishes with all known signal types (see review in Crampton & Albert, 2006). These included those with EODs containing low frequencies, near the frequency sensitivity of small loudspeakers (e.g. Electrophorus electricus and Sternopygus macrurus, and also those with EODs containing high frequencies, such as Hypopygus lepturus and Sternarchella schotti. The EFF will also clearly amplify and broadcast the weak EODs of small post-larval gymnotiform fishes (e.g. ca. 20 mm Gymnotus carapo where the peak-to-peak amplitude is < 10 mVcm-1@10 cm).
Circuit Design. The circuit plan is illustrated in Fig. 1, with stages A - E annotated (see below). A list of components is provided in Table 1. The signal enters the amplifier via the grounded dipole electrode input. A 3-pin Lemo input is preferred for its extremely rugged construction and water resistance, although a 3-pin microphone connector would suffice. In stage A the signal is differentially amplified 100 times and converted to a single ended output. Stage B provides an optional 5 x gain (x 500 total) only when the switch is set to high-gain. Stage C provides a further 20 x gain to drive the magnetic loud-speaker. Section D provides the ground for the circuit by dividing the voltage difference of V+ and V- between two equal value resistors. The node between the resistors can thus be considered a reference or 0V. It is important to select two 12K resistors whose resistance lies within 2% of each other (the value need not be precisely 12K). This will ensure that the reference will be precisely centered between V+ and V-. The 22uF capacitor between V+ and V- eliminates large fluctuations in the supply voltage. If there is a sudden demand for current and the battery cannot supply this fast enough, stored charge in the capacitor will provide the deficit, thus preventing a precipitous voltage drop. Section E illustrates the 3-way toggle-switch schematic. No calibration or maintenance of the circuit is required. A standard electronic textbook (e.g. Sinclair, 2005) is all that is needed to follow the circuit plan.
Construction. The EFF is built in an aluminum box (152 x 83 x 50 mm) with an external black polymer coating and hermetically sealed removable lid (Fig. 2). Holes are drilled at opposite ends of the box for the dipole connector and toggle-switch. All components are soldered to the main circuit board and connected with circuit wire. An alternative approach is to have the circuit board professionally etched and each component soldered in place. This allows several boards to be made automatically, and reduces assembly effort, but does not result in improved performance. The finished circuit board is glued to the base of the box at each corner with large beads of clear silicone sealing rubber. These rubberized attachments protect the circuit board and its components from physical shock if the unit is dropped. Should the board need to be repaired, these attachments can be pulled off and remade.
Holes drilled in a pattern on the lid allow sound from the loudspeaker to exit the box. The speaker is glued using silicone sealant to the lid. The Mylar polymer cone of the loudspeaker and the silicone sealant prevent water entering the box via the holes. The leads to the loudspeaker should be long enough to allow the lid to be taken off and placed aside the box during battery changes. The battery bay is lined with a layer of packing foam to provide a tight fit for the 9V batteries. The dipole socket and toggle switch are water resistant. The Jig-latch bolts for the swivels are each secured in position with a hex nut, locking washer and a bonded steel/rubber sealing washer to provide a watertight seal.
Tests demonstrated that no water will enter the unit during heavy rain or splashing, or during complete submersion for ten seconds. For normal field use (avoiding any more than momentary accidental submersion) a small sachet of silica gel kept inside the box will keep the components completely dry. This sachet can be recharged at the end of each day in the field. Should the unit be submerged for longer it should be opened and if necessary dried in strong sunlight and then left for 6 hours in a desiccating chamber.
Operation and probe types. For most field applications, the fish finder is connected via a grounded dipole connector to a 2 2.5 m microphone cable (Figs. 3, 4). The two channels corresponding to the differential inputs in Stage A are stripped from the main body of the end of the cable to the desired length and left in their plastic shields, exposing only the terminal 2-3 mm of metal as contacts with the water. The grounded shield (which wraps around the channels) is exposed, cut, and twisted to form a small stub near the main cable where the two channels depart (Fig. 3). The combination of the two exposed contacts and the ground is hereafter called the 'dipole'.
For locating electric fishes in marginal roots and vegetation, or leaf litter banks of small streams, the separated channels of the dipole can be firmly attached with duct tape to the end of a straight pole or cut branch (Fig. 4). The terminals should be separated by approximately 10 15 cm, with the ground in any position that will be submerged at the same time as the exposed wires (somewhere in the middle is preferable). The position of a gymnotiform can be determined in the field by sweeping movements of the dipole, with the EFF switched to the high-gain setting. On locating a fish, the EFF should then be switched to the low-gain setting to allow a determination of its exact position by more confined movements of the dipole. In low-conductivity water (5 20 µScm-1) a 15 cm pulse-type gymnotiform (e.g. a Gymnotus) is typically detectable at a range of up to 3 m with the high-gain setting, and 1 m with the low-gain setting. In higher conductivity water these ranges are reduced. Following detection and localization, gymnotiforms can be captured using a dip net (Fig. 4). Typically the net brings up sand, mud and organic material. The net can be lifted from the water and briefly touched with the dipole to determine if the fish is in the net or not. A machete is often required to cut away dense vegetation or root mats in order to dislodge gymnotiforms.
In very shallow water (< 10 cm) the dipole terminals are best mounted at the ends of the prongs of a forked stick. The ground should be positioned near the end of one of the prongs to ensure that it is also submerged. For locating electric fishes in dense rafts of floating macrophytes, the best results are achieved by attaching the two channels to the ends of two parallel prongs mounted on the end of a pole. Such a fork can be constructed from narrow PVC tubing. The fish finder can also be used to detect gymnotiform fishes in deep water by utilizing a long shielded microphone cable, with the electrode contacts attached to a weighted cable. To avoid damage by stretching or abrasion, the microphone cable should be attached to the weighted cable with duct tape or cable ties at 1 m intervals. Tests in the Amazon River in Peru confirmed that the fish finder described here can readily detect the signals of gymnotiforms at depths of 50 m, or more, permitting surveys of even the deepest sections of large rivers.
Acknowledgements
This material is based upon work supported by the National Science Foundation (NSF) under Grants 0614334 and 0724280 to WGRC (Principal Investigator). SW received funding from a NSF Research Experience for Undergraduates summer internship (Grant 0724280). We thank J. Mol, A. Caputi, and L. Verdi for assistance in the field, and two anonymous reviewers.
Literature Cited
Submitted April 2007
Accepted August 2007
- Crampton, W. G. R. & J. S. Albert. 2006. Evolution of electric signal diversity in gymnotiform fishes. I. Phylogenetic systematics, ecology and biogeography. Pp. 647-696, 718-731. In: Ladich, F., S. P. Collin, P. Moller & B. G Kapoor (Eds.). Communication in Fishes. Enfield, N.H. Science Publishers Inc.
- Sinclair, I. R. 2005. Practical Electronics Handbook. Oxford, Newnes Press.
- Wells, K. & W. G. R. Crampton. 2006. A portable bio-amplifier for electric fish research: design and construction. Neotropical Ichthyology 4(2): 295-299.
Publication Dates
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Publication in this collection
29 Oct 2007 -
Date of issue
Sept 2007
History
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Received
Apr 2007 -
Accepted
Aug 2007