Summary
The importance of studying hemoglobin behaviour in fish is discussed, noting the exceptional oportunity for this aboard RV Alpha Helix. Several examples are given of ligand binding under equilibria. Several possibilities for further research are also discussed, such as association and dissociation in ligand binding, the phenomenon of regularized phases, fluctuations of protein conformation, division of the molecule between two or more ligands and the effect of organic phosphates and other substances. Also discussed are ligands in resting state and under transition, as well as the possibility of using antigenic reactions in phylogenetic research.