Copaiba oleoresin presents anti-obesogenic effect and mitigates inflammation and redox imbalance in adipose tissue

TELLES, Luciana Ortega; SILVA, Bianca Sulzbacher da; PAULINO, Angélica Macedo Borges; MENDONÇA, Sabrina Trigueiro; SINHORIN, Valéria Dornelles Gindri; LIMA, Milena Campelo Freitas de; VEIGA JUNIOR, Valdir Florêncio; ANDRIGHETTI, Carla Regina; NASCIMENTO, André Ferreira do; BOMFIM, Gisele Facholi; LUVIZOTTO, Renata Azevedo Melo

doi:10.1590/1809-4392202201411

Luciana Ortega TELLES

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Bianca Sulzbacher da SILVA

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Angélica Macedo Borges PAULINO

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Sabrina Trigueiro MENDONÇA

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Valéria Dornelles Gindri SINHORIN

Universidade Federal de Mato Grosso (UFMT), Instituto de Ciências Naturais, Humanas e Sociais, Sinop, MT, Brazil

Milena Campelo Freitas de LIMA

Universidade Federal do Amazonas (UFAM), Departamento de Química, Manaus, AM, Brazil

Valdir Florêncio VEIGA JUNIOR

Instituto Militar de Engenharia, Departamento de Engenharia Química, Rio de Janeiro, RJ, Brazil

Carla Regina ANDRIGHETTI

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

André Ferreira do NASCIMENTO

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Gisele Facholi BOMFIM

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

Renata Azevedo Melo LUVIZOTTO ^** Corresponding author: renata.nascimento@ufmt.br

Universidade Federal de Mato Grosso (UFMT), Instituto Ciências da Saúde, Sinop, MT, Brazil

https://orcid.org/0000-0001-9548-6338

* Corresponding author: renata.nascimento@ufmt.br

ASSOCIATE EDITOR:

Emiliano Barreto

Figures (2)
Tables (4)

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Figure 1
Protein concentration of cytokines in epididymal adipose tissue of rats subjected to three treatments for eight weeks. C: control; S: sucrose solution; S+OC: sucrose solution supplemented with copaiba oleoresin (200 mg kg^-1 day^-1). A - Interleukin (IL)-6; B - IL-1β; C - IL-10. Columns represent the mean and bars the standard deviations, n = 8. Different letters above the bars indicate significant differences between groups according to a Student-Newman-Keuls test. This figure is in color in the electronic version.

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Figure 2
Determination of redox status in epididymal adipose tissue of rats subjected to three treatments for eight weeks. C: control; S: sucrose solution; S+OC: sucrose solution supplemented with copaiba oleoresin (200 mg kg^-1 day^-1). A - reduced glutathione (GSH); B - glutathione S-transferase (GST); C - superoxide dismutase (SOD); D - ascorbic acid (Vit C); E - protein carbonylation; F - thiobarbituric acid reactive substances (TBARS). Columns represent the mean and bars the standard deviations, n = 8. Different letters above the bars indicate significant differences between groups according to a Student-Newman-Keuls test.

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Table 1
Nutritional composition of the diet fed to the rats.

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Table 2
Chemical composition of copaiba (Copaifera sp.) oleoresin from Cotriguaçu, Mato Grosso state, Brazil, determined by HRGC-MS analyses.

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Table 3
Caloric intake and morphological characteristics of rats subjected to three treatments for eight weeks. C: control; S: sucrose solution; S+OC: sucrose solution supplemented with copaiba oleoresin (200 mg kg^-1 day^-1). Values are the mean ± SD, n = 8. Different superscript letters in a line indicate significant differences between groups according to a Student-Newman-Keuls test. The caloric intake includes energy from sucrose in the drinking water (1.2 kcal mL^-1) in the S and S+CO groups.

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Table 4
Plasma values of fasting glucose and lipid profile of rats subjected to three treatments for eight weeks. C: control; S: sucrose solution; S+OC: sucrose solution supplemented with copaiba oleoresin (200 mg kg^-1 day^-1). HDL: high density lipoprotein; LDL: low density lipoprotein; VLDL: very low-density lipoprotein; TG: triglycerides. Values are the mean ± SD, n = 8. Different superscript letters in a line indicate significant differences between groups according to a Student-Newman-Keuls test.

Components	Commercial chow Nuvilab® Cr-1
Carbohydrate (g kg^-1)	655
Protein (g kg^-1)	220
Fat (g kg^-1)	40
Fibers (g kg^-1)	40
Vitamin mixture (g kg^-1)	10
Mineral mixture (g kg^-1)	35
Total (g kg^-1)	1000
Energy (kcal g^-1)	3.8

Compound	Retention index	Concentration (%)
α-cubebene	1348	0.49
Cyclosativene	1369	0.28
α-copaene	1374	8.26
β-cubebene	1387	2.02
β-elemene	1389	4.10
Cyperene	1398	0.24
β-caryophyllene	1417	0.49
trans-α-bergamotene	1432	20.24
cis-β-farnesene	1440	1.27
epi-β-santalene	1445	0.29
α-humulene	1452	0.09
trans-β-farnesene	1454	1.09
allo-aromadendrene	1458	0.61
4,5-di-epi-aristolochene	1471	0.13
γ-muurolene	1478	0.13
Germacrene D	1480	1.86
β-selinene	1489	11.48
α-selinene	1498	5.69
cis-α-bisabolene	1506	1.17
β-bisabolene	1505	8.80
7-epi-α-selinene	1520	0.38
δ-cadinene	1522	2.06
Junenol	1618	0.19
Selin-11-en-4-α-ol	1658	1.08
epi-α-bisabolol	1683	0.12
β-copaen-4-α-ol	1590	0.39
Kaur-16-en acid methyl ester	-	0.45
Kaurenoic acid methyl ester	-	7.80
Copalic acid methyl ester	-	1.78
Daniellic acid methyl ester	-	0.58
Identified constituents (%)		83.55
Sesquiterpene content (%)		72.94
Diterpenes (%)		10.60

Variable	Treatments
Variable	C	S	S+CO
Body weight initial (g)	323 ± 23^a	323 ± 24^a	323 ± 26^a
Body weight final (g)	448 ± 25^a	498 ± 24^b	444 ± 28^a
Body weight gain (g)	125 ± 21^a	175 ± 24^b	121 ± 37^a
Caloric intake (kcal day^-1)	101 ± 1.9^a	108 ± 7.1^b	105 ± 0.9^b
Epididymal fat (g)	9.84 ± 0.9^a	12.8 ± 3.5^a	10.1 ± 2.4^a
Mesenteric fat (g)	6.18 ± 0.8^a	12.9 ± 3.2^b	6.84 ± 2.4^a
Retroperitoneal fat (g)	14.1 ± 1.8^a	19.5 ± 3.5^b	13.6 ± 3.8^a
Visceral fat (g)	30.1 ± 3.0^a	45.3 ± 9.3^b	30.7 ± 7.2^a
Adiposity index (%)	6.82 ± 0.6^a	9.26 ± 1.6^b	6.95 ± 1.3^a

Variable	Treatments
Variable	C	S	S+CO
Fasting glucose (mg dL^-1)	97.4 ± 12^a	112 ± 9^b	105 ± 6^ab
Total cholesterol (mg dL^-1)	184 ± 66^a	306 ± 117^b	227 ± 61^ab
HDL (mg dL^-1)	31.6 ± 11^a	32.9 ± 10^a	31.1 ± 8^a
LDL (mg dL^-1)	91 ± 41^a	172 ± 71^b	126 ± 41^ab
VLDL (mg dL^-1)	60.9 ± 22^a	102 ± 39^b	69.8 ± 23^a
Triglycerides (mg dL^-1)	304 ± 109^a	508 ± 194^b	349 ± 115^a
TG/HDL ratio	10.3 ± 3.2^a	17.1 ± 4.3^b	12.6 ± 2.1^a

[1] * Corresponding author: renata.nascimento@ufmt.br

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Copaiba oleoresin presents anti-obesogenic effect and mitigates inflammation and redox imbalance in adipose tissue

ABSTRACT