Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

Recent studies have suggested that therapies with stem cells and amniotic membrane can modulate the inflammation following an ischemic injury in the heart. This study evaluated the effects of bone-marrow mononuclear cells (BMMC) and acellular human amniotic membrane (AHAM) on cardiac function and NLRP3 complex in a rat model of heart failure.On the 30th day,the echocardiographic showed improvements on ejection fraction and decreased pathological ventricular remodeling on BMMC and AHAM groups.Oxidative stress analysis was similar between the three groups,and the NLRP3 inflammasome activity were not decreased with the therapeutic use of both BMMC and AHAM,in comparison to the control group.

Key words
heart failure; Bone-marrow mononuclear cells; Amniotic membrane; NLRP3 inflammasome; oxidative stress

Authorship

ALINE L. TAKEJIMA

drafting and revising the manuscript

final approval of the version to be published.

Pontifícia Universidade Católica do Paraná (PUCPR), Experimental Laboratory of Institute of Biological and Health Sciences, 1555 Imaculada Conceição Street, 80215-901 Curitiba, PR, BrazilPontifícia Universidade Católica do Paraná (PUCPR)BrazilCuritiba, PR, BrazilPontifícia Universidade Católica do Paraná (PUCPR), Experimental Laboratory of Institute of Biological and Health Sciences, 1555 Imaculada Conceição Street, 80215-901 Curitiba, PR, Brazil

https://orcid.org/0000-0002-2608-0171

PAULO ANDRÉ B. MACHADO-JÚNIOR

acquisition

analysis and interpretation of data

https://orcid.org/0000-0003-1577-9195

GUSTAVO G. BLUME

acquisition

analysis and interpretation of data

https://orcid.org/0000-0002-2936-8541

ROSSANA BAGGIO SIMEONI

acquisition

analysis and interpretation of data

https://orcid.org/0000-0002-9976-5809

JULIO CESAR FRANCISCO

drafting and revising the manuscript

final approval of the version to be published

https://orcid.org/0000-0003-1970-6399

MURILO S. TONIAL

acquisition

analysis and interpretation of data

https://orcid.org/0000-0001-6302-7014

LUIS FELIPE B. MARQUEZE

acquisition

analysis and interpretation of data

Pontifícia Universidade Católica do Paraná (PUCPR), Laboratory of Exercise Biochemistry in Health, School of Medicine, 1555 Imaculada Conceição Street, Prado Velho, 80215-901 Curitiba, PR, BrazilPontifícia Universidade Católica do Paraná (PUCPR)BrazilCuritiba, PR, BrazilPontifícia Universidade Católica do Paraná (PUCPR), Laboratory of Exercise Biochemistry in Health, School of Medicine, 1555 Imaculada Conceição Street, Prado Velho, 80215-901 Curitiba, PR, Brazil

https://orcid.org/0000-0002-4738-1440

LUCIA NORONHA

acquisition

analysis and interpretation of data

https://orcid.org/0000-0003-0310-7164

MARCIA OLANDOSKI

acquisition

analysis and interpretation of data

https://orcid.org/0000-0002-0979-3604

ELTYEB ABDELWAHID

drafting and revising the manuscript

final approval of the version to be published

Northwestern University, Feinberg School of Medicine, Feinberg Cardiovascular Research Institute, 303 E. Chicago Ave., Tarry 14-725, 60611 Chicago, IL, USANorthwestern UniversityUSAChicago, IL, USANorthwestern University, Feinberg School of Medicine, Feinberg Cardiovascular Research Institute, 303 E. Chicago Ave., Tarry 14-725, 60611 Chicago, IL, USA

https://orcid.org/0000-0002-2964-6927

KATHERINE A.T. DE CARVALHO

drafting and revising the manuscript

final approval of the version to be published

The Pelé Pequeno Príncipe Institute, Cell Therapy and Biotechnology in Regenerative Medicine Department, Child and Adolescent Health Research & Pequeno Príncipe Faculties, 1632 Silva Jardim Ave., Água Verde, 80240-020 Curitiba, PR, BrazilThe Pelé Pequeno Príncipe InstituteBrazilCuritiba, PR, BrazilThe Pelé Pequeno Príncipe Institute, Cell Therapy and Biotechnology in Regenerative Medicine Department, Child and Adolescent Health Research & Pequeno Príncipe Faculties, 1632 Silva Jardim Ave., Água Verde, 80240-020 Curitiba, PR, Brazil

https://orcid.org/0000-0003-4860-2855

RICARDO A. PINHO

drafting and revising the manuscript

final approval of the version to be published

https://orcid.org/0000-0003-3116-4553

LUIZ CÉSAR GUARITA-SOUZA

drafting and revising the manuscript

final approval of the version to be published

https://orcid.org/0000-0003-2781-9705

Correspondence to: Aline Luri Takejima E-mail: alinetakejima@yahoo.com.br

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (5)

Thumbnail

Figure 1
Comparison of the echocardiography results on the 7th and 30th days after myocardial infarction. The results are presented as mean +/- standard deviation. p<0,05 denoted statistical significance versus control group (#) or intragroup baseline analysis (*). Sample size: control = 8; BMMC = 11; AHAM = 8. EF (ejection fraction, in %); LVESV (left ventricle end-systolic volume, in mL); LVEDV (left ventricle end-diastolic volume, in mL); BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Thumbnail

Figure 2
Effects of BMMC and AHAM on inflammatory parameters 30 days after myocardial infarction. The results of TNF-α (a) and NF-kβ (b) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance versus the control group (*). Sample size: control = 8; BMMC = 11; AHAM = 8. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Thumbnail

Figure 3
Effects of BMMC and AHAM on oxidative stress parameters after 30 days of myocardial infarction. The values of DCF (a), total GSH (b), GSSG (c) and GSH/GSSG ratio (d) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance. Sample size: control = 8; BMMC = 11; AHAM = 8. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Thumbnail

Figure 4
Effects of BMMC and AHAM on the NLRP3 inflammasome activity 30 days after myocardial infarction. The values of NALP3 (a), ASC (b), Caspase-1 (c) and interleukin-1β (d) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance. Sample size for ASC, Caspase-1, NALP3 and interleukin- 1β: control = 8; BMMC = 11; AHAM = 8.

Thumbnail

Figure 5
Representative images of histological analysis (cross sections) of the hearts of animals subjected to myocardial infarction. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane). Acquisition of images in 10x objective.

Figure 1 Comparison of the echocardiography results on the 7th and 30th days after myocardial infarction. The results are presented as mean +/- standard deviation. p<0,05 denoted statistical significance versus control group (#) or intragroup baseline analysis (*). Sample size: control = 8; BMMC = 11; AHAM = 8. EF (ejection fraction, in %); LVESV (left ventricle end-systolic volume, in mL); LVEDV (left ventricle end-diastolic volume, in mL); BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Figure 2 Effects of BMMC and AHAM on inflammatory parameters 30 days after myocardial infarction. The results of TNF-α (a) and NF-kβ (b) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance versus the control group (*). Sample size: control = 8; BMMC = 11; AHAM = 8. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Figure 3 Effects of BMMC and AHAM on oxidative stress parameters after 30 days of myocardial infarction. The values of DCF (a), total GSH (b), GSSG (c) and GSH/GSSG ratio (d) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance. Sample size: control = 8; BMMC = 11; AHAM = 8. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane).

Figure 4 Effects of BMMC and AHAM on the NLRP3 inflammasome activity 30 days after myocardial infarction. The values of NALP3 (a), ASC (b), Caspase-1 (c) and interleukin-1β (d) are presented as mean +/- standard deviation. p<0,05 denoted statistical significance. Sample size for ASC, Caspase-1, NALP3 and interleukin- 1β: control = 8; BMMC = 11; AHAM = 8.

Figure 5 Representative images of histological analysis (cross sections) of the hearts of animals subjected to myocardial infarction. BMMC (bone marrow mononuclear cells); AHAM (acellular human amniotic membrane). Acquisition of images in 10x objective.

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Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure

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[1] Correspondence to: Aline Luri Takejima E-mail: alinetakejima@yahoo.com.br