Molecular characterization of the progenetic metacercariae<i> Crocodilicola pseudostoma</i> parasitizing <i>Rhamdia quelen </i>(Siluriformes, Heptapteridae) in Brazil

NEGRELLI, DÉBORA C.; VIEIRA, DIEGO H.M.D.; ABDALLAH, VANESSA D.; AZEVEDO, RODNEY K.

doi:10.1590/0001-3765202020181388

DÉBORA C. NEGRELLI

Universidade Estadual Paulista “Júlio de Mesquita Filho”/UNESP, Instituto de Biociências, Departamento de Bioestatística, Biologia Vegetal, Parasitologia e Zoologia, Setor Parasitologia, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, 18618-689 Botucatu, SP, Brazil

https://orcid.org/0000-0001-7907-8609

DIEGO H.M.D. VIEIRA

Universidade Estadual Paulista “Júlio de Mesquita Filho”/UNESP, Instituto de Biociências, Departamento de Bioestatística, Biologia Vegetal, Parasitologia e Zoologia, Setor Parasitologia, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, 18618-689 Botucatu, SP, Brazil

https://orcid.org/0000-0002-6137-1353

VANESSA D. ABDALLAH

Centro Universitário CESMAC, Rua Prof. Ângelo Neto, 50, 57051-530 Maceió, AL, Brazil

https://orcid.org/0000-0001-6539-6091

RODNEY K. AZEVEDO

Centro Universitário CESMAC, Rua Prof. Ângelo Neto, 50, 57051-530 Maceió, AL, Brazil

https://orcid.org/0000-0002-0471-6079

Correspondence to: Rodney Kozlowiski de Azevedo E-mail: azevedork@gmail.com

AUTHOR CONTRIBUTIONS

Negrelli DC performed sampling analysis, study design, analyzed data, including morphometric data. Vieira DHMD performed the analysis and writing corresponding to molecular biology and phylogenetic analysis. Abdallah VD & Azevedo RK identified the parasite specimen, collaborated throughout the study, reviewed and were responsible for the development of the manuscript. All authors contributed to the writing and all stages of this manuscript.

		Present study	Ferrari-Hoeinghaus et al. (2007)FERRARI-HOEINGHAUS A, TAKEMOTO RM & PAVANELLI GC. 2007. Digenetic trematode parasites of Loricariichthys platymetopon (Loricariidae, Siluriformes) of the upper Paraná river floodplain, Brazil. Acta Sci Biol Sci 29: 327-329.	Armas de Conroy (1986)ARMAS DE CONROY G. 1986. Crocodilicola pseudostoma (Willemoes-Suhm, 1870) Poche, 1925 (Trematoda: Proterodiplostomatidae), endoparásito del bagre pimelódido Rhamdia hilarii Val., 1840 del Estado de São Paulo, Brasil. Rev Ibér Parasitol 46: 35-38.
Site of infection		Encysted in the liver and free in the swimming bladder, cavity, intestine and stomach	Digestive tract	Body cavity
Host		Rhamdia quelen	Loricariichthys platymetopon	Rhamdia hilarii
Locality		Jacaré-Pepira River, Brazil	Upper Paraná River floodplain, Brazil	Instituto de Pesca, Brazil
Body	Length	1250.0 – 3000.0 (2000.0) µm	2075.0 – 2575.0 (2364.0) µm	1336.0 – 1721.0 µm
Body	Width	250.0 – 580.0 (490.0) µm	500.0 – 800.0 (662.0) µm	722.0 – 845.0 µm
Oral sucker	Length	60.0 – 89.0 (71.0) µm	60.0 – 70.0 (64.0) µm	61.8 – 148.0 µm
Oral sucker	Width	54.0 – 86.0 (74.0) µm	50.0 – 70.0 (58.0) µm	63.0 – 80.0 µm
Pharynx	Length	61.0 – 112.0 (80.0) µm	50.0 – 70.0 (56.0) µm	54.8 – 64.8 µm
Pharynx	Width	36.0 – 60.0 (48.0) µm	50.0 – 60.0 (55.0) µm	43.8 – 53.8 µm
Acetabulum	Length	100.0 – 150.0 (122.0) µm	110.0 – 150.0 (124.0) µm	102.4 – 112.8 µm
Acetabulum	Width	116.0 – 170.0 (136.0) µm	110.0 – 190.0 (145.0) µm	132.2 – 185.6 µm
Tribocytic organ	Length	140.0 – 252.0 (212.0) µm	170.0 – 260.0 (206.0) µm	50.4 – 78.0 µm
Tribocytic organ	Width	93.0 – 189.0 (136.0) µm	150.0 – 230.0 (182.0) µm	98.0 – 147.2 µm
Eggs	Length	99.0 – 131.0 (116.0) µm	80.0 – 120.0 (96.0) µm	111.2 – 124.0 µm
Eggs	Width	43.0 – 74.0 (60.0) µm	50.0 – 80.0 (74.0) µm	54.4 – 68.0 µm

Parasite	Host	Locality	Reference
(MF398317) Crocodilicola pseudostoma	Rhamdia guatemalensis	Mexico	Hernández-Mena et al. 2017HERNÁNDEZ-MENA DI, GARCÍA-VARELA M & LEÓN GPP. 2017. Filling the gaps in the classification of the Digenea Carus, 1863: systematic position of the Proterodiplostomidae Dubois, 1936 within the superfamily Diplostomoidea Poirier, 1886, inferred from nuclear and mitochondrial DNA sequences. Syst Parasitol 94: 833-848.
(MF398318) Crocodilicola pseudostoma	Rhamdia guatemalensis	Mexico	Hernández-Mena et al. 2017HERNÁNDEZ-MENA DI, GARCÍA-VARELA M & LEÓN GPP. 2017. Filling the gaps in the classification of the Digenea Carus, 1863: systematic position of the Proterodiplostomidae Dubois, 1936 within the superfamily Diplostomoidea Poirier, 1886, inferred from nuclear and mitochondrial DNA sequences. Syst Parasitol 94: 833-848.
(GQ292490) Diplostomum huronense	Multiple species	Canada	Locke et al. 2010aLOCKE SA, MCLAUGHLIN JD, DAYANANDAN S & MARCOGLIESE DJ. 2010a. Diversity and specificity in Diplostomum spp. metacercariae in freshwater fishes revealed by cytochrome c oxidase I and internal transcribed spacer sequences. Int J Parasitol 40: 333-343.
(FJ477196) Diplostomum indistinctum	-	Canada	Moszczynska et al. 2009MOSZCZYNSKA A, LOCKE SA, MCLAUGHLIN JD, MARCOGLIESE DJ & CREASE TJ. 2009. Development of primers for the mitochondrial cytochrome c oxidase I gene in digenetic trematodes (Platyhelminthes) illustrates the challenge of barcoding parasitic helminths. Mol Ecol Resour 9: 75-82.
(HM064679) Diplostomum sp.	Multiple species	Canada	Locke et al. 2010bLOCKE SA, MCLAUGHLIN JD & MARCOGLIESE DJ. 2010b. DNA barcodes show cryptic diversity and a potential physiological basis for host specificity among Diplostomoidea (Platyhelminthes: Digenea) parasitizing freshwater fishes in the St. Lawrence River, Canada. Mol Ecol 19: 2813-2827.
(JQ639170) Diplostomum pseudospathaceum	Perca fluviatilis	Germany	Behrmann-Godel 2013BEHRMANN-GODEL J. 2013. Parasite identification, succession and infection pathways in perch fry (Perca fluviatilis): new insights through a combined morphological and genetic approach. Parasitology 140: 509-520.
(JX986909) Tylodelphys clavata	Perca fluviatilis	Germany	Georgieva et al. 2013GEORGIEVA S, SOLDÁNOVÁ M, PÉREZ-DEL-OLMO A, DANGEL RD, SITKO J, SURES B & KOSTADINOVA A. 2013. Molecular prospecting for European Diplostomum (Digenea: Diplostomidae) reveals cryptic diversity. Int J Parasitol 43: 57-72.
(KC685329) Tylodelphys mashonensis	Clarias gariepinus	Tanzania	Chibwana et al. 2013CHIBWANA FD, BLASCO-COSTA I, GEORGIEVA S, HOSEA KM, NKWENGULILA G, SCHOLZ T & KOSTADINVOA A. 2013. A first insight into the barcodes for African diplostomids (Digenea: Diplostomidae): Brain parasites in Clarias gariepinus (Siluriformes: Clariidae). Infect Genet Evol 17: 62-70.
(KM115882) Austrodiplostomum ostrowskiae	Cichlasoma trimaculatum	Mexico	García-Varela et al. 2015GARCÍA-VARELA M, SERENO-URIBE AL, PINACHO-PINACHO CD, DOMÍNGUEZ-DOMÍNGUEZ O & PÉREZ-PONCE DE LEÓN G. 2015. Molecular and morphological characterization of Austrodiplostomum ostrowskiae Dronen, (Digenea: Diplostomatidae), a parasite of cormorants in the Americas. J Helminthol 90: 174-185.
(KR271494) Tylodelphys jenynsiae	Cnesterodon decemmaculatus	South America	Locke et al. 2015LOCKE AS ET AL. 2015. Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes. Int J Parasitol 45: 841-855.
(FJ477223) Tylodelphys scheuringi	-	Canada	Moszczynska et al. 2009MOSZCZYNSKA A, LOCKE SA, MCLAUGHLIN JD, MARCOGLIESE DJ & CREASE TJ. 2009. Development of primers for the mitochondrial cytochrome c oxidase I gene in digenetic trematodes (Platyhelminthes) illustrates the challenge of barcoding parasitic helminths. Mol Ecol Resour 9: 75-82.
(KR271481) Tylodelphys immer	Multiple species	South America	Locke et al. 2015LOCKE AS ET AL. 2015. Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes. Int J Parasitol 45: 841-855.
(JX977780) Apharyngostrigea cornu	Nyctycorax nyctycorax	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(HM064887) Apaharyngostrigea pipientis	-	Canada	Locke et al. 2010bLOCKE SA, MCLAUGHLIN JD & MARCOGLIESE DJ. 2010b. DNA barcodes show cryptic diversity and a potential physiological basis for host specificity among Diplostomoidea (Platyhelminthes: Digenea) parasitizing freshwater fishes in the St. Lawrence River, Canada. Mol Ecol 19: 2813-2827.
(JX977731) Parastrigea diovadena	Eudocimus albus	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(JX977776) Parastrigea plataleae	Platalea ajaja	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(JX977760) Parastrigea cincta	Eudocimus albus	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(JX977781) Cotylurus gallinulae	Aythya affinis	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(KY513232) Cotylurus cornutus	Gyraulus acronicus	Norway	Soldánová et al. 2017SOLDÁNOVÁ M ET AL. 2017. Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake. Int J Parasitol 47: 327-345.
(KY513232) Cotylurus cornutus	Radix balthica	Norway	Soldánová et al. 2017SOLDÁNOVÁ M ET AL. 2017. Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake. Int J Parasitol 47: 327-345.
(KT831347) Cotylurus gallinulae	Physella gyrina	Canada	Gordy et al. 2016GORDY MA, KISH L, TARRABAIN M & HANINGTON PC. 2016. A comprehensive survey of larval digenean trematodes and their snail hosts in central Alberta, Canada. Parasitol Res 115: 3867-3880.
(JX977784) Cardiocephaloides sp.	Larus occidentalis	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(JX977782n) Cardiocephaloides medioconiger	Larus sp.	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.
(JX977782) Cardiocephaloides medioconiger	Larus sp.	Mexico	Hernández-Mena et al. 2014HERNÁNDEZ-MENA DI, GARCÍA-PRIETO, L & GARCÍA-VARELA M. 2014. Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63: 315-323.

[1] Correspondence to: Rodney Kozlowiski de Azevedo E-mail: azevedork@gmail.com

Brasil

Brasil

Molecular characterization of the progenetic metacercariae Crocodilicola pseudostoma parasitizing Rhamdia quelen (Siluriformes, Heptapteridae) in Brazil

Abstract