In the articleDiosmin induces caspase-dependent apoptosis in human glioblastoma cells, with DOI number:http://dx.doi.org/10.1590/0001-3765201920191031, published in the journalAnais da Academia Brasileira de Ciências, 91(4): e20191031, page 9
Reads:
Figure 4
Diosmin induced morphological alterations and reduced GBM95 and GBM02 cellularity. Phase contrast (gray scale) and vimentin immunostaining (red) were used to evaluate cell morphology and cytoskeleton. DAPI (blue) was used for nuclei staining to count the total number of cells. GBM95 and GBM02 were treated with diosmin (at 50 and 100 μM) for 24 and 48 hours. DMSO was used as control. Phase contrast and vimentin representative images of GBM95 (a) and GBM02 (b), representative graphs of the length (micrometers) of GBM95 (c) and GBM02 (e), and representative graphs of total number of GBM95 (d) and GBM02 (f) cells are shown. Results represent mean ± SEM of three independent experiments, each performed in duplicate (*p <0.05; **p <0.01; ***p <0.001). Scale bar: 50 μm.
Diosmin induced morphological alterations and reduced GBM95 and GBM02 cellularity. Phase contrast (gray scale) and vimentin immunostaining (red) were used to evaluate cell morphology and cytoskeleton. DAPI (blue) was used for nuclei staining to count the total number of cells. GBM95 and GBM02 were treated with diosmin (at 50 and 100 μM) for 24 and 48 hours. DMSO was used as control. Phase contrast and vimentin representative images of GBM95 (a) and GBM02 (b), representative graphs of the length (micrometers) of GBM95 (c) and GBM02 (e), and representative graphs of total number of GBM95 (d) and GBM02 (f) cells are shown. Results represent mean ± SEM of three independent experiments, each performed in duplicate (*p <0.05; **p <0.01; ***p <0.001). Scale bar: 50 μm.
Should read:
Figure 4
Diosmin induced morphological alterations and reduced GBM95 and GBM02 cellularity. Phase contrast (gray scale) and vimentin immunostaining (red) were used to evaluate cell morphology and cytoskeleton. DAPI (blue) was used for nuclei staining to count the total number of cells. GBM95 and GBM02 were treated with diosmin (at 50 and 100 μM) for 24 and 48 hours. DMSO was used as control. Phase contrast and vimentin representative images of GBM95 (a) and GBM02 (b), representative graphs of the length (micrometers) of GBM95 (c) and GBM02 (e), and representative graphs of total number of GBM95 (d) and GBM02 (f) cells are shown. Results represent mean ± SEM of three independent experiments, each performed in duplicate (*p <0.05; **p <0.01; ***p <0.001). Scale bar: 50 μm.
Diosmin induced morphological alterations and reduced GBM95 and GBM02 cellularity. Phase contrast (gray scale) and vimentin immunostaining (red) were used to evaluate cell morphology and cytoskeleton. DAPI (blue) was used for nuclei staining to count the total number of cells. GBM95 and GBM02 were treated with diosmin (at 50 and 100 μM) for 24 and 48 hours. DMSO was used as control. Phase contrast and vimentin representative images of GBM95 (a) and GBM02 (b), representative graphs of the length (micrometers) of GBM95 (c) and GBM02 (e), and representative graphs of total number of GBM95 (d) and GBM02 (f) cells are shown. Results represent mean ± SEM of three independent experiments, each performed in duplicate (*p <0.05; **p <0.01; ***p <0.001). Scale bar: 50 μm.
Publication Dates
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Publication in this collection
09 Mar 2020 -
Date of issue
2020
