Toxicity of commercial atrazine in rattus novergicus organs as a function of concentration: histopathological, ultrastructural and hematological evaluation

RIERA, JOSÉ; MATUS, ERICKA; MATUS, LORENA; MOLINO, JAY

doi:10.1590/0001-3765202220201125

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CELLULAR AND MOLECULAR BIOLOGY • An. Acad. Bras. Ciênc. 94 (2) • 2022 • https://doi.org/10.1590/0001-3765202220201125 copy

Toxicity of commercial atrazine in rattus novergicus organs as a function of concentration: histopathological, ultrastructural and hematological evaluation

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The effect of commercial Atrazine (ATR) on Rattus Novergicus organs was determined for a concentration of c_ATR of 1, 3, 13, 30, and 50 ppb. ATR was dissolved in drinking water. The rats were allowed to drink from it ad libitum for an uninterrupted period of 28 days as established by the Office of Environmental Protection protocol under the number EPA OPPTS 870.3050. In the 28 days of the test, data on the behavior of the medicated animals was recorded before the extraction of sample tissues (heart, liver, spleen, brain, and testicles) for histological analysis. A direct correlation of c_ATR and organ damage was found. The study showed that even for the smallest doses (1ppb), commercial ATR produced several lesions in the studied animals. The rats showed hepatic periacinar necrosis with acute coagulation, hepatocyte lipidosis, severe portal lymphocytic inflammation, coronary periarteritis, and lymphocytic meningoencephalitis for high concentrations. In the male reproductive system, testicular degeneration with mild acute tubular necrosis was observed.

Key words
Atrazine; microscope alterations; River la Villa; organ damage; herbicide; toxicity

	1 µg L ^-1						3 µg L ^-1						\|10 µg L ^-1						30 µg L ^-1						50 µg L ^-1
	♀			♂			♀			♂			♀			♂			♀			♂			♀			♂
DAYS	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14	0	7	14
Head	193	204	218	185			199	200	214	200	217	215	200	205	210	207	197	201	200	204	215	204	210	220	200	205		207
Foreleg	190	185	215	195	200	215	204	197	221	195	199	214	197	220	209	199	200	190	204	185	212	220	229	233	207	215	224	204	203	209
Back	197	202	224	200	217	227	200	207	210	212	220	232	203	227	213	200	205	212	209	202	219	217	225	232	211	219	228	211	207	221
Hind leg	200	197	217	205	208	216	218	216	230	204	216	220	211	215	220	209	213	224	211	197	221	212	220	229	213	220	232	205	210	222
Tail				193	201	216	197	210	214	199	206	217	207	220	215	197	207	211	207	220	215	200	212	218	217	223	228	202	212	221

Parameter	Control group	1 µg L^-1	3 µg L^-1	13 µg L^-1	30 µg L^-1	50 µg L^-1
Red Blood Cells	8020 mm³	8020 mm³	8020 mm³	7500 mm³	7210 mm³	6550 mm³
Hemoglobin	12.54 gr	12.50 gr	12.50 gr	12.00 gr	11.45 gr	11,20 gr
Hematocrit	39.2 %	39.10 %	39.10 %	38.40 %	37.50%	37,10 %
MCHC	31.98 gr dl^-1	31.98 gr dl^-1	31.80 gr dl^-1	30.10 gr dl^-1	28.75 gr dl^-1	28,40 gr dl^-1
Segmented Neutrophils	40 %	40 %	37 %	35 %	34 %	33 %
Lymphocytes	60 %	60 %	63 %	65 %	66 %	67 %
Total bilirubin	0.995 mg dl^-1	0.995 mg dl^-1	0.995 mg dl^-1	0.995 mg dl^-1	0.995 mg dl^-1	0,995 mg dl^-1
Direct bilirubin	0.78 mg dl^-1	0.78 mg dl^-1	0.78 mg dl^-1	0.78 mg dl^-1	0.78 mg dl^-1	0,78 mg dl^-1
Indirect bilirubin	0.33 mg dl^-1	0.33 mg dl^-1	0.33 mg dl^-1	0.33 mg dl^-1	0.33 mg dl^-1	0,33 mg dl^-1
Total protein	8.05 gr dl^-1	8.05 gr dl^-1	8.05 gr dl^-1	8.05 gr dl^-1	9.10 gr dl^-1	9,25 gr dl^-1
Albumin	3.7 gr dl^-1	3.7 gr dl^-1	3.7 gr dl^-1	3.90 gr dl^-1	3.90 gr dl^-1	4,10 gr dl^-1
Globulin	4.3 gr dl^-1	4.3 gr dl^-1	4.3 gr dl^-1	4.80 gr dl^-1	4.80 gr dl^-1	5,00 gr dl^-1
A/G ratio	0.86	0.86	0.86	0.81	0.81	0,86
TGO	24 Ul	24 Ul	24 Ul	27 Ul	29 Ul	35 Ul
Tryglicerides	124 mg dl^-1	124 mg dl^-1	124 mg dl^-1	155 mg dl^-1	170 mg dl^-1	180 mg dl^-1
Cholesterol	129 mg dl^-1	129 mg dl^-1	129 mg dl^-1	145 mg dl^-1	155 mg dl^-1	165 mg dl^-1
Glycemia	142 mg dl^-1	142 mg dl^-1	142 mg dl^-1	160 mg dl^-1	165 mg dl^-1	160 mg dl^-1
Urea	100 mg dl^-1	100 mg dl^-1	110 mg dl^-1	115 mg dl^-1	120 mg dl^-1	125 mg dl^-1
Creatinine	0.94 mg dl^-1	0.94 mg dl^-1	0.94 mg dl^-1	1.10 mg dl^-1	1.15 mg dl^-1	1,20 mg dl^-1
Uric acid	4.0 mg dl^-1	4.0 mg dl^-1	4.0 mg dl^-1	4.15 mg dl^-1	4.75 mg dl^-1	5,10 mg dl^-1
TGP	22 µL	27 µL	28 µL	28 µL	30 µL	35 µL

		Hb	TG	HDL	Glycemia	UA	TGP
N		6	6	6	6	6	6
	Mean	12.0317	146.17	142.00	151.83	4.3333	28.33
	Stdev	.58789	25.553	15.582	10.926	0.47504	4.227
Máximas diferencias extremas	Absolute	.287	0.307	0.298	0.316	0.317	0.210
	Positive	.194	0.307	0.298	0.316	0.317	0.198
	Negative	-.287	-0.193	-0.202	-0.273	-0.241	-0.210
Test Statistic		.287	-0.307	0.298	0.316	0.317	0.210
Asymptotic significance (bilateral)		.133^a	-0.080^a	0.104 ^a	0.062^a	0.060^a	0.200^a,^b
Significance		0.080^b	0.080	0.104^b	0.062^b	0.060^b	0.200

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[1] Correspondence to: Jay Molino E:mail: jay.molino@udelas.ac.pa