Corallus hortulanus testes histology: morphological and reproductive aspects

In general snakes show differentiate anatomical, biological and behavioral particularities compared to other species. Basic information about the snakes anatomy, physiology and reproductive biology is scarce in several species, making the reproduction a challenge. Thus, the present work aims to evaluate morphological aspects of the Corallus hortulanus testes, correlating these findings with environmental factors and reproductive aspects. The testes of three specimens of Corallus hortulanus were cut to a thickness of 3μm in microtome, stained with 1% toluidine blue, photo documented and described. Seasonality was observed in the sperm production of Corallus hortulanus, with the presence of mature spermatozoa in the wettest and hottest periods of the year, as well as the largest testicular volume in these periods.

Key words
snake; reproduction; spermatogenesis; testicular morphometry

Authorship

HEITOR JOSÉ BENTO

preparation of the study

writing and reviewing the manuscript

sample processing

analysis and interpretation of data

Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal de Mato Grosso - UFMT, Faculdade de Medicina Veterinária, Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, BrazilUniversidade Federal de Mato Grosso - UFMTBrazilCuiabá, MT, BrazilPrograma de Pós-Graduação em Ciências Veterinárias, Universidade Federal de Mato Grosso - UFMT, Faculdade de Medicina Veterinária, Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, Brazil

Universidade Federal de Mato Grosso - UFMT, Laboratório de Pesquisa em Animais de Zoológico (LPAZ), Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, Brazil Universidade Federal de Mato Grosso - UFMTBrazilCuiabá, MT, BrazilUniversidade Federal de Mato Grosso - UFMT, Laboratório de Pesquisa em Animais de Zoológico (LPAZ), Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, Brazil

https://orcid.org/0000-0002-8670-1515

ADELINA FERREIRA

preparation of the study

writing and reviewing the manuscript

analysis and interpretation of data

Universidade Federal de Mato Grosso - UFMT, Instituto de Biociências, Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, BrazilUniversidade Federal de Mato Grosso - UFMTBrazilCuiabá, MT, BrazilUniversidade Federal de Mato Grosso - UFMT, Instituto de Biociências, Av. Fernando Correa da Costa, 2367, Boa Esperança, 78060-900 Cuiabá, MT, Brazil

https://orcid.org/0000-0002-7999-4946

GABRIELLA A. IGLESIAS

sample processing

https://orcid.org/0000-0001-6578-0456

TATIANE G. DE ARAÚJO

analysis and interpretation of data.

Programa de Pós-graduação em Estatística Aplicada e Biometria, Universidade Federal de Alfenas - PPGEAB-UNIFAL, Rua Gabriel Monteiro da Silva, 700, Centro, 37130-001 Alfenas, MG, BrazilUniversidade Federal de Alfenas - PPGEAB-UNIFALBrazilAlfenas, MG, BrazilPrograma de Pós-graduação em Estatística Aplicada e Biometria, Universidade Federal de Alfenas - PPGEAB-UNIFAL, Rua Gabriel Monteiro da Silva, 700, Centro, 37130-001 Alfenas, MG, Brazil

https://orcid.org/0000-0002-0784-1828

FELIPE F. CURCIO

sample processing

https://orcid.org/0000-0003-1200-5354

REGINA CELIA R. DA PAZ

preparation of the study

writing and reviewing the manuscript

https://orcid.org/0000-0003-4567-0043

Correspondence to: Heitor José Bento E-mail: heitorjbento@gmail.com

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (3)

Thumbnail

Figure 1
Rainfall (mm) and temperature (°C) average of capture period in Weather Station Salto do Céu/A936 (specimens 7490), Weather Station Porto Estrela/A935 (specimens 11123) and Weather Station Cotriguaçu/A919 (specimen 9577). The arrow indicates the capture moment. (Source: BDMEP-INMET).

Thumbnail

Figure 2
Corallus hortulanus testicle histology (a) - Specimen 7490 (stage 6), captured in high temperatures and rainfall period. Seminiferous tubules with sperm (arrowhead), and absence of cellular remains inside. Cells in different phases of gametogenesis in the germ epithelium. Toluidine blue 1%, obj. 40x. (b) - Specimen 11123 (stage 6), captured in high rainfall and temperatures. Seminiferous tubules with evident lumen, with sperm (arrowhead) and cellular debris (arrow) inside. The germ epithelium presents cells in different phases of gametogenesis, and evident interstitial tissue with interstitial Leydig cells. Toluidine blue 1%, obj. 40x. (c) - Specimen 9577 (stage 8), captured in a lower rainfall and temperature period. Testicles with few seminiferous tubules, lumen absence, no sperm and cellular remains inside. Germ cells in apoptosis process after the reproductive period is observed in the seminiferous tubules, which are characterized by homogeneous aspect, with condensed chromatin and cytoplasm in fragmentation and collapse, no evident Sertoli cells. Interstitial tissue is evident, with interstitial Leydig cells grouped. Toluidine blue 1%, obj. 40x. Legend: (S) Sertoli cells, (E1) spermatogonia type A, (E2) spermatogonia type B, (ES1) primary spermatocyte, (ES2) secondary spermatocyte, (EP) spermatid, (ST) seminiferous tubule, (*) Leydig interstitial cells.

Thumbnail

Figure 3
Corallus hortulanus morphometric analysis in different reproductive periods: germinal epithelium height and seminiferous tubules diameter. The boxes represent the mean values (middle line) and standard deviation, and the lines above and below represent the upper and lower bounds, respectively.

Figure 1 Rainfall (mm) and temperature (°C) average of capture period in Weather Station Salto do Céu/A936 (specimens 7490), Weather Station Porto Estrela/A935 (specimens 11123) and Weather Station Cotriguaçu/A919 (specimen 9577). The arrow indicates the capture moment. (Source: BDMEP-INMET).

Figure 2 Corallus hortulanus testicle histology (a) - Specimen 7490 (stage 6), captured in high temperatures and rainfall period. Seminiferous tubules with sperm (arrowhead), and absence of cellular remains inside. Cells in different phases of gametogenesis in the germ epithelium. Toluidine blue 1%, obj. 40x. (b) - Specimen 11123 (stage 6), captured in high rainfall and temperatures. Seminiferous tubules with evident lumen, with sperm (arrowhead) and cellular debris (arrow) inside. The germ epithelium presents cells in different phases of gametogenesis, and evident interstitial tissue with interstitial Leydig cells. Toluidine blue 1%, obj. 40x. (c) - Specimen 9577 (stage 8), captured in a lower rainfall and temperature period. Testicles with few seminiferous tubules, lumen absence, no sperm and cellular remains inside. Germ cells in apoptosis process after the reproductive period is observed in the seminiferous tubules, which are characterized by homogeneous aspect, with condensed chromatin and cytoplasm in fragmentation and collapse, no evident Sertoli cells. Interstitial tissue is evident, with interstitial Leydig cells grouped. Toluidine blue 1%, obj. 40x. Legend: (S) Sertoli cells, (E1) spermatogonia type A, (E2) spermatogonia type B, (ES1) primary spermatocyte, (ES2) secondary spermatocyte, (EP) spermatid, (ST) seminiferous tubule, (*) Leydig interstitial cells.

Figure 3 Corallus hortulanus morphometric analysis in different reproductive periods: germinal epithelium height and seminiferous tubules diameter. The boxes represent the mean values (middle line) and standard deviation, and the lines above and below represent the upper and lower bounds, respectively.

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Corallus hortulanus testes histology: morphological and reproductive aspects

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[1] Correspondence to: Heitor José Bento E-mail: heitorjbento@gmail.com