Antiproliferative effect of guava fruit extracts in MDA-MB-435 and MCF-7 human breast cancer cell lines

CORREA, MARIANA G.; COUTO, JESSICA S.; TRINDADE, BRUNO B.; ABREU, JOEL P.; NAKAJIMA, VANIA M.; OLIVEIRA, FELIPE L.; FARAH, ADRIANA; TEODORO, ANDERSON J.

doi:10.1590/0001-3765202020191500

MARIANA G. CORREA

Universidade Federal do Estado do Rio de Janeiro, Departamento de Ciência dos Alimentos, Laboratório de Alimentos Funcionais (LAAF), Av. Pasteur, 296, 22290-240 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0003-4778-858X

JESSICA S. COUTO

Universidade Federal do Estado do Rio de Janeiro, Departamento de Ciência dos Alimentos, Laboratório de Alimentos Funcionais (LAAF), Av. Pasteur, 296, 22290-240 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0001-9484-2388

BRUNO B. TRINDADE

Universidade Federal do Estado do Rio de Janeiro, Departamento de Ciência dos Alimentos, Laboratório de Alimentos Funcionais (LAAF), Av. Pasteur, 296, 22290-240 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0003-4871-5817

JOEL P. ABREU

Universidade Federal do Estado do Rio de Janeiro, Departamento de Ciência dos Alimentos, Laboratório de Alimentos Funcionais (LAAF), Av. Pasteur, 296, 22290-240 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0003-2994-7823

VANIA M. NAKAJIMA

Universidade Federal Fluminense, Departamento de Nutrição e Dietética, Rua Mário Santos Braga, 28, 24110-010 Niterói, RJ, Brazil

https://orcid.org/0000-0003-0705-0708

FELIPE L. OLIVEIRA

Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Av Carlos Chagas Filho, 393, 21941-902 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0001-6193-1616

ADRIANA FARAH

Universidade Federal do Rio de Janeiro, Instituto de Nutrição, Av. Carlos Chagas Filho, 373, 21941-902 Cidade Universitária, Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0002-7584-5564

ANDERSON J. TEODORO

Universidade Federal do Estado do Rio de Janeiro, Departamento de Ciência dos Alimentos, Laboratório de Alimentos Funcionais (LAAF), Av. Pasteur, 296, 22290-240 Rio de Janeiro, RJ, Brazil

https://orcid.org/0000-0002-0949-9528

Correspondence to: Anderson Junger Teodoro E-mail: atteodoro@gmail.com

AUTHOR CONTRIBUTIONS

Conceptualization, M.G.C. and A.J.T.; methodology, M.G.C., J.S.C., B.B.T., J.P.A., F.L.O. and A.J.T.; data curation, M.G.C., J.S.C., B.B.T., J.P.A., F.L.O., V.M.N., A.F. and A.J.T.; writing—original draft preparation, M.G.C., J.S.C., B.B.T., J.P.A., F.L.O., V.M.N., A.F. and A.J.T.; writing—review and editing, M.G.C., J.S.C., B.B.T., J.P.A., F.L.O., V.M.N., A.F. and A.J.T.

Figures (5)
Tables (2)

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Figure 1
Effect of Tsumori (TS), Pedro Sato (PS), and Hitígio (HI) guava extracts (15-5000µg /mL) on MDA-MB -435 cells viability after 24 and 48 hrs treatment. Results were compared by One- way ANOVA test and Tukey test (* p<0.05; ** p <0.01). CT= Results of untreated (CT) and treated cells.

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Figure 2
Effect of 24 and 48 hrs incubation of MCF-7 cells with Tsumori (TS), Pedro Sato (PS) and Hitígio (HI) guava cultivar extracts (15-5000μg /mL) on cells viability. Results were compared by One- way ANOVA followed by Tukey test Significant differences are indicated as * p<0.05 and ** p <0.01.

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Figure 3
Effect of 48h incubation of MDA-MB 435 cells with 1000 and 5000 μg/ mL Pedro Sato (PS), Tsumori (TS) and Hitígio (HI) guava cultivar extracts on cell cycle progression. Cell cycle phases are illustrated in (a). Quantitative results are shown in graph (b). Results are expressed as mean ± standard deviation of two experiments. Significant differences between untreated (CT) and treated cells were compared by One- way ANOVA followed byTukey test Significant differences are indicated as * p<0.05 and ** p <0.01.

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Figure 4
Effect of 24hrs-incubation of MCF-7 cells with 1000 and 5000 μg/ mL of Pedro Sato (PS), Tsumori (TS) and Hitígio (HI) guava cultivars extracts on cell cycle progression. Cell cycle phases are illustrated in (a). Quantitative results are shown in graph (b). Results are expressed as mean ± standard deviation of two experiments. Results of untreated (CT) and treated cells were compared by One- way ANOVA followed by Tukey test. Significant differences are indicated as * p<0.05 and ** p <0.01.

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Figure 5
Increased relative rate of apoptosis in MDA-MB 435(a and b) treated for 48 hrs, with 1000 and 5000 μg/ mL of Pedro Sato (PS), Tsumori (TS) and Hitigio (HI) guava cultivars extracts. Results were compared by One- way ANOVA followed by Tukey test for significance (* p<0.05; p <0.01). CT= Results of untreated (CT) and treated cells.

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Table I
Physical and chemical characterization of guava cultivar extracts. Significant differences are indicated as a, b and c.

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Table II
Effect of Pedro Sato(PS), Tsumori(TS) and Hitigio(HI) guava cultivars extracts on death process stages in MDA-MB-435 and MCF-7. Significant differences are indicated as *, **p<0.01, ***p<0.001.

Physical parametres				Chemical and antioxidant parameters
	PS	TS	HI		PS	TS	HI
Weight (g)	220.16±40.08^a	297.52±79.06^c	158.16±15.43^b	Total sugar (g%)	12.33±0.45^a	13.12±1.73^a	10.95±.1.22^a
Dimension (cm)	8.34±0.51^a	8.78±1.01^c	7.27±0.40^b	Soluble Solids (°Brix)	10.11±0.02^a	11.08±0.01^a	9.47±0.01^a
CIELAB scale (peel)				Titratable Acidity (g%)	0.36±0.01^a	0.47±0.03^b	0.39±0.01^a
L*	65.56±1.72^a	55.49±10.06^b	59.90±3.71^a.^b	Ascorbic acid (mg%)	81.81±2.04^a	80.36±1.87 ^a	79.35±3.30^a
a*	-0.40±2.22^b	0.90±4.59^b	-5.18±2.32^a	Lycopene (mg/100g)	5.43±0.32^a	2.34±0.43^c	3.21±0.85^b
b*	42.30±3.16^a	28.59±9.12^b	39.59±3.08^a	β-carotene (mg/100g)	0.13±0.08^a	1.54±0.98^c	0.50±0.12^b
CIELAB scale (pulp)				Total phenolics (mg GAE/100 g)	200.88±1.44^a	125.56±8.74^c	156.64±1.14^b
L*	55.21±1.68^a	61.83±4.59^a	58.53±1.72^a	DPPH (% reduction)	76.96±2.35	56.84±1.04	55.91 ±0.80
a*	32.25±1.17^a	22.45±1.97^b	30.99±1.24^a	ABTS (µmol Trolox/g)	208.05±9.69	151.07±1.93	129.91±3.04
b*	21.69±2.02^a	21.01±1.16^a	21.81±0.51^a	FRAP (µmol ferrous sulfate/g)	41.84±0.84	30.41±0.46	23.73±0.20

		MDA-MB 435			MCF-7
Guava Cultivars extract	Stages of cell death	CT	1000µg/mL	5000µg/mL	CT	1000µg/mL	5000µg/mL
PS	Viable cells (Annexin V- PI-)	86.03±3.21	39.10±4.10***	44.25±1.77**	93.60±1.34	89.75±1.64*	73.55±10.92**
	Early apoptosis (Annexin V+ PI-)	3.77±1.88	46.25±0.21***	17.85±0.96***	0.84±0.78	4.29±0.39**	8.58±3.69***
	Late apoptosis (Annexin V+ PI+)	3.81±0.99	16.75±0.35***	36.15±1.20***	0.25±0.57	2.93±0.11*	3.90±0.86*
	Non-apoptotic cells (Annexin V- PI+)	6.42±1.52	0.61±0.16***	2.25±1.06**	4.29±1.13	5.15±1.90	5.36±0.78
TS	Viablecells (Annexin V- PI-)	82.60±4.81	39.10±4.20**	44.25±1.77**	91.85±2.19	90.30±1.45	90.35±1.34
	Early apoptosis (Annexin V+ PI-)	3.27±0.76	17.58±2.39***	2.20±1.70	2.51±0.78	1.77±0.39	0.94±0.34*
	Late apoptosis (Annexin V+ PI+)	5.05±1.80	16.75±2.33***	36.15±1.20***	3.57±0.85	2.38±0.30	2.87±1.90
	Non-apoptotic cells (Annexin V- PI+)	9.06±3.73	1.03±0.63***	2.25±1.06**	4.04±1.06	7.15±1.90*	8.87±2.60*
HI	Viable cells (Anexin V- PI-)	80.28±1.35	47.45±9.00**	31.15±1.83***	92.10±4.12	81.35±4.74*	83.70±2.38*
	Early apoptosis (Annexin V+ PI-)	2.73±1.74	42.55±19.16**	1.30±1.00	3.78±1.47	7.62±2.52**	8.24±0.72**
	Late apoptosis (Anexin V+ PI+)	6.32±3.20	9.78±2.01	50.55±0.78***	2.42±2.62	7.98±0.57**	6.36±1.25**
	Non-apoptotic cells (Annexin V- PI+)	11.72±2.98	0.30±0.16***	13.55±3.64	3.71±1.21	2.08±1.64	2.94±0.98

[1] Correspondence to: Anderson Junger Teodoro E-mail: atteodoro@gmail.com

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Antiproliferative effect of guava fruit extracts in MDA-MB-435 and MCF-7 human breast cancer cell lines

Abstract