Mycological Diversity Description I

Here, Quambalaria fabacearum and Neopestalotiopsis brasiliensis are introduced as new species from Brazil, isolated as endophyte from Mimosa tenuiflora and causing post-harvest rot disease on fruits of Psidium guajava, respectively. Diaporthe inconspicua is emended to include a more detailed morphological description. Neopestalotiopsis egyptiaca is reported as new to the Americas and as causing post-harvest rot disease on fruits of Psidium guajava, while Umbelopsis isabellina is reported as endophyte.

Keywords:
Amphisphaeriales; CaM; Diaporthales; H3; ITS rDNA; LSU rDNA; Microstromatales; tef1-α; TUB2; Umbelopsidales

Authorship

Jadson Diogo Pereira Bezerra ^** Corresponding author: JDPB: jadsondpb@gmail.com, OLP: oliparini@ufv.br

Departamento de Micologia, Universidade Federal de Pernambuco, 50670-901, Recife, PE, BrazilUniversidade Federal de PernambucoBrazilRecife, PE, BrazilDepartamento de Micologia, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil

https://orcid.org/0000-0002-7782-8602

Alexandre Reis Machado

https://orcid.org/0000-0001-7440-3097

André Luiz Firmino

Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, 38500-000, Monte Carmelo, MG, BrazilUniversidade Federal de UberlândiaBrazilMonte Carmelo, MG, BrazilInstituto de Ciências Agrárias, Universidade Federal de Uberlândia, 38500-000, Monte Carmelo, MG, Brazil

https://orcid.org/0000-0003-4632-9584

André Wilson Campos Rosado

Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, BrazilUniversidade Federal de ViçosaBrazilViçosa, MG, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil

https://orcid.org/0000-0003-4781-2033

Carlos Alberto Fragoso de Souza

https://orcid.org/0000-0002-8388-4897

Cristina Maria de Souza-Motta

https://orcid.org/0000-0002-0964-8271

Karla Torres Lins de Sousa Freire

https://orcid.org/0000-0002-4692-5258

Laura Mesquita Paiva

https://orcid.org/0000-0001-9720-9887

Oliane Maria Correia Magalhães

https://orcid.org/0000-0001-7275-5304

Olinto Liparini Pereira ^** Corresponding author: JDPB: jadsondpb@gmail.com, OLP: oliparini@ufv.br

https://orcid.org/0000-0002-0274-4623

Pedro W. Crous

Westerdijk Fungal Biodiversity Institute, 3508 AD, Utrecht, The Netherlands Westerdijk Fungal Biodiversity InstituteThe NetherlandsUtrecht, The NetherlandsWesterdijk Fungal Biodiversity Institute, 3508 AD, Utrecht, The Netherlands

https://orcid.org/0000-0001-9085-8825

Thays Gabrielle Lins de Oliveira

https://orcid.org/0000-0001-5415-3916

Vanessa Pereira de Abreu

Departamento de Microbiologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil Universidade Federal de ViçosaBrazilViçosa, MG, BrazilDepartamento de Microbiologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil

https://orcid.org/0000-0002-4708-3814

Xinlei Fan

The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, 100083, Beijing, ChinaBeijing Forestry UniversityChinaBeijing, ChinaThe Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, 100083, Beijing, China

https://orcid.org/0000-0002-4946-4442

* Corresponding author: JDPB: jadsondpb@gmail.com, OLP: oliparini@ufv.br

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (9)

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Figure 1
Quambalaria fabacearum (URM 7756 - ex-type culture). A. Culture on PDA. B. Culture on MEA. C. Detail of the colony on MEA. D. Conidiophores and conidia. E. Conidia forming acropetal chains. F. Primary conidia forming secondary conidia. Scale bars: D-E: 10 µm; F: 5 µm.

Thumbnail

Figure 2
Bayesian Inference tree obtained from data set of 15 taxa including representatives of genera Quambalaria and a potential new species comparing ITS rDNA and LSU rDNA (concatenated) with Microstroma phylloplanum (Microstromatales) as outgroup.

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Figure 3
Diaporthe inconspicua (URM 7775). A-B. Culture on PDA after 15d. C-D. Pycnidial conidiomata on PDA after 30d. E-J. Conidiophores, conidiogenous cells and beta conidia. L. Alpha conidia. M. Beta conidia. N. Alpha and beta conidia. Scale bars: 10 µm.

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Figure 4
Bayesian Inference tree obtained from concatenated dataset of ITS rDNA, tef1-α and TUB2 sequences of 24 species of the genera Diaporthe with Diaporthella corylina (CBS 121124) as outgroup. Posterior probabilities from BI, ML and MP bootstrap support values above 70 %, respectively, are shown at nodes. Strains obtained in the current study are in green.

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Figure 5
Neopestalotiopsis brasiliensis (COAD 2166 - ex-type culture). A. Colony on MEA after seven days at 25 ºC. B. Colony on PDA after seven days at 25 ºC. C. Conidiomata sporulating on PDA. D. Conidial masses. E. Conidia formation in vitro. F. Detail of conidial formation in vitro (arrow). G. Conidium. H. Pathogenicity test seven days after inoculation. Scale bars: D: 50µm; E-G: 20µm.

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Figure 6
Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined sequences of the ITS rDNA, TUB2 and tef1-α. The Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Pseudopestalotiopsis theae MFLUCC 12-0055. The species in this study are highlighted in bold. Neopestalotiopsis spp. Clade 4, Clade 10, Clade 15, Clade 20 and Clade 26 is according to Maharachchikumbura et al. (2014Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW. 2014. Pestalotiopsis revisited. Studies in Mycology 79: 121-186.).

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Figure 7
Neopestalotiopsis egyptiaca (COAD 2167). A. Colony on MEA after seven days at 25 ºC. B. Colony on PDA after seven days at 25 ºC. C. Conidioma sporulating on PDA. D. Conidial masses. E. Conidiogenous cells. F. Detail of annellidic conidiogenous cell (arrow). G. Conidium. H. Pathogenicity test seven days after inoculation. Scale bars: D: 50µm; E, G: 20µm; F: 10µm.

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Figure 8
Umbelopsis isabellina (URM 7862). A. Colony surface on MEA after seven days. B. Simple sporangiophore with sporangium. C. Branched simple sporangiophores arising from the same point. D. Simple sporangiophores with inconspicuous columellae (arrows). E. Sporangiospores. Scale bars: 16, 25, 30 and 10 µm, respectively.

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Figure 9
Maximum Likelihood (ML) tree obtained with MEGA 7 using ITS rDNA sequences of Umbelopsis species with Spinellus fusiger (CBS 405.63) and Phycomyces blakesleeanus (CBS 270.32) as outgroups. ML bootstrap values above 60 % are shown at nodes. Isolates obtained in the current study are in green.

Figure 1 Quambalaria fabacearum (URM 7756 - ex-type culture). A. Culture on PDA. B. Culture on MEA. C. Detail of the colony on MEA. D. Conidiophores and conidia. E. Conidia forming acropetal chains. F. Primary conidia forming secondary conidia. Scale bars: D-E: 10 µm; F: 5 µm.

Figure 2 Bayesian Inference tree obtained from data set of 15 taxa including representatives of genera Quambalaria and a potential new species comparing ITS rDNA and LSU rDNA (concatenated) with Microstroma phylloplanum (Microstromatales) as outgroup.

Figure 3 Diaporthe inconspicua (URM 7775). A-B. Culture on PDA after 15d. C-D. Pycnidial conidiomata on PDA after 30d. E-J. Conidiophores, conidiogenous cells and beta conidia. L. Alpha conidia. M. Beta conidia. N. Alpha and beta conidia. Scale bars: 10 µm.

Figure 4 Bayesian Inference tree obtained from concatenated dataset of ITS rDNA, tef1-α and TUB2 sequences of 24 species of the genera Diaporthe with Diaporthella corylina (CBS 121124) as outgroup. Posterior probabilities from BI, ML and MP bootstrap support values above 70 %, respectively, are shown at nodes. Strains obtained in the current study are in green.

Figure 5 Neopestalotiopsis brasiliensis (COAD 2166 - ex-type culture). A. Colony on MEA after seven days at 25 ºC. B. Colony on PDA after seven days at 25 ºC. C. Conidiomata sporulating on PDA. D. Conidial masses. E. Conidia formation in vitro. F. Detail of conidial formation in vitro (arrow). G. Conidium. H. Pathogenicity test seven days after inoculation. Scale bars: D: 50µm; E-G: 20µm.

Figure 6 Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined sequences of the ITS rDNA, TUB2 and tef1-α. The Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Pseudopestalotiopsis theae MFLUCC 12-0055. The species in this study are highlighted in bold. Neopestalotiopsis spp. Clade 4, Clade 10, Clade 15, Clade 20 and Clade 26 is according to **Maharachchikumbura et al. (2014**Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW. 2014. Pestalotiopsis revisited. Studies in Mycology 79: 121-186.).

Figure 7 Neopestalotiopsis egyptiaca (COAD 2167). A. Colony on MEA after seven days at 25 ºC. B. Colony on PDA after seven days at 25 ºC. C. Conidioma sporulating on PDA. D. Conidial masses. E. Conidiogenous cells. F. Detail of annellidic conidiogenous cell (arrow). G. Conidium. H. Pathogenicity test seven days after inoculation. Scale bars: D: 50µm; E, G: 20µm; F: 10µm.

Figure 8 Umbelopsis isabellina (URM 7862). A. Colony surface on MEA after seven days. B. Simple sporangiophore with sporangium. C. Branched simple sporangiophores arising from the same point. D. Simple sporangiophores with inconspicuous columellae (arrows). E. Sporangiospores. Scale bars: 16, 25, 30 and 10 µm, respectively.

Figure 9 Maximum Likelihood (ML) tree obtained with MEGA 7 using ITS rDNA sequences of Umbelopsis species with Spinellus fusiger (CBS 405.63) and Phycomyces blakesleeanus (CBS 270.32) as outgroups. ML bootstrap values above 60 % are shown at nodes. Isolates obtained in the current study are in green.

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E-mail: acta@botanica.org.br

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[1] * Corresponding author: JDPB: jadsondpb@gmail.com, OLP: oliparini@ufv.br