Mycological Diversity Description II

Here, Diaporthe myracrodruonis is introduced as new species from Brazil, isolated as endophyte from Myracrodruon urundeuva. Asterina mandaquiensis is epitypified and ilustrated for the first time. Serpula similis is reported as new to the Neotropics, while Perenniporia centrali-africana is reported for the first time as endophyte and Preussia africana as endophyte from Spondias tuberosa in Caatinga in Brazil.

Keywords:
Asterinales; Boletales; CaM; Diaporthales; ITS rDNA; LSU rDNA; Pleosporales; Polyporales; tef1-α; TUB2

Authorship

Rejane Maria Ferreira da Silva ^** Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com

Departamento de Micologia, Universidade Federal de Pernambuco, 50740-600, Recife, PE, Brazil Universidade Federal de PernambucoBrazilRecife, PE, BrazilDepartamento de Micologia, Universidade Federal de Pernambuco, 50740-600, Recife, PE, Brazil

https://orcid.org/0000-0002-5024-0286

Adriene Mayra Soares ^** Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com

https://orcid.org/0000-0002-1143-0706

Ana Patrícia Sousa Lopes de Pádua

https://orcid.org/0000-0002-0058-8831

André Luiz Firmino ^** Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com

Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, 38500-000, Monte Carmelo, MG, BrazilUniversidade Federal de UberlândiaBrazilMonte Carmelo, MG, BrazilInstituto de Ciências Agrárias, Universidade Federal de Uberlândia, 38500-000, Monte Carmelo, MG, Brazil

https://orcid.org/0000-0003-4632-9584

Cristina Maria de Souza-Motta

https://orcid.org/0000-0002-0964-8271

Gladstone Alves da Silva

https://orcid.org/0000-0002-5652-7800

Helio Longoni Plautz Jr

Laboratório de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110, Belém, PA, BrazilUniversidade Federal do ParáBrazilBelém, PA, Brazil Laboratório de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110, Belém, PA, Brazil

https://orcid.org/0000-0002-8030-4420

Jadson Diogo Pereira Bezerra ^** Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com

https://orcid.org/0000-0002-7782-8602

Laura Mesquita Paiva

https://orcid.org/0000-0001-9720-9887

Leif Ryvarden

Institute of Biological Sciences, University of Oslo, N-0316, Oslo, NorwayUniversity of OsloNorwayOslo, Norway Institute of Biological Sciences, University of Oslo, N-0316, Oslo, Norway

https://orcid.org/0000-0002-4670-7306

Luisa Cunha Oliani

Laboratório de Microbiologia, Universidade Federal do Vale do São Francisco, 56304-917, Petrolina, PE, BrazilUniversidade Federal do Vale do São FranciscoBrazilPetrolina, PE, Brazil Laboratório de Microbiologia, Universidade Federal do Vale do São Francisco, 56304-917, Petrolina, PE, Brazil

https://orcid.org/0000-0001-5613-0419

Mayara Alice Correia de Mélo

https://orcid.org/0000-0003-2980-4080

Oliane Maria Correia Magalhães

https://orcid.org/0000-0001-7275-5304

Olinto L. Pereira

Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, BrazilUniversidade Federal de ViçosaBrazilViçosa, MG, Brazil Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil

https://orcid.org/0000-0002-0274-4623

Rafael José Vilela de Oliveira

https://orcid.org/0000-0002-8854-9449

Tatiana Baptista Gibertoni

https://orcid.org/0000-0003-4499-7650

Thays Gabrielle Lins de Oliveira

https://orcid.org/0000-0001-5415-3916

Virginia Michelle Svedese

https://orcid.org/0000-0001-6487-7651

Xinlei Fan

The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, 100083, Beijing, ChinaBeijing Forestry UniversityChinaBeijing, China The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, 100083, Beijing, China

https://orcid.org/0000-0002-4946-4442

* Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com

SCIMAGO INSTITUTIONS RANKINGS

Institute of Biological Sciences, University of Oslo, N-0316, Oslo, NorwayUniversity of OsloNorwayOslo, Norway Institute of Biological Sciences, University of Oslo, N-0316, Oslo, Norway

Figures

Figures (10)

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Figure 1
Perenniporia centrali-africana. A-B. Culture on PDA after 10 days (B. reverse); C. Skeletal hyphae. D-F. Chlamydospores-like structures. G-H. Generative hyphae with clamp connection (arrowed). Scale bars: 10 µm.

Thumbnail

Figure 2
Phylogenetic tree of the Perenniporia constructed using ITS rDNA sequences, showing the position of P. centrali-africana isolated from watermelon leaves. The sequence obtained in this study is in boldface. Support values are from Bayesian inference and maximum likelihood analyses, respectively.

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Figure 3
Serpula similis(URM 89847): A. Fresh basidiomata. B. Hymenial Surface. C. Generative hyphae with clamp connections. D. Yellow basidisopores. Scale bars: a, b=10 cm. c, d= 5 μm. Figure: A.M.S. Soares and R. Alvarenga. The macro- (morphology, color, consistency, size) analyses were performed on both fresh and dehydrated basidiomata. Free-hand sections from hymenium and context were visualized under optical microscopy using 3 % KOH + 1 % phloxine, and Melzer’s reagent (Ryvarden 1991Ryvarden L. 1991. Genera of polypores, nomenclature and taxonomy. Synopsis Fungorum 5: 1-363.) Color designation followed Watling (1969Watling R. 1969. Colour identification chart. Edinburgh, Her Majesty`s Stationery Office.).

Thumbnail

Figure 4
Phylogenetic tree inferred from ITS and LSU rDNA gene sequences using Bayesian and maximum likelihood analyses with the GTR + G evolution model retrieved by PartitionFinder v1.1.0 (Lanfear et al. 2012Lanfear R, Calcott B, Ho SYW, Guindon S. 2012. PartitionFinder: combined selection of partitioning schemes. Molecular Biology and Evolution 29: 1695-1701.). Bayesian and likelihood trees showed the same topology displayed here. Branch lengths reflect estimated number of 0.2 changes per site. Bayesian analysis was performed with MrBayes 3.2.1 software (Ronquist et al. 2012Ronquist F, Teslenko M, Mark P, et al. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 1-4.) for 10 million generations with four Markov chains. Likelihood analysis was performed with the same evolutionary model under 1000 rearrangements using RAxML (Stamatakis 2014Stamatakis A. 2014. Raxml version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312-1313.). Bootstrap support (BS) values after 1000 rearrangements and posterior probabilities are indicated on tree nodes. Branches with PP > 0.95 and BS from 90 % are considered as strongly supported.

Thumbnail

Figure 5
Asterina mandaquiensis (VIC42824, epitype). A. Colonies on leaf surface. B. Colony with open thyriothecia and surface mycelium. C. Thyriothecia opened by a central star-shaped fissure. D. Thyriothecium fringed at margins. E. Branched mycelium with appressoria. F. Alternate to unilateral appressoria. G. Ovoid ascus with immature hyaline ascospores. H. Brown and smooth mature bicelular ascospores. Scale bars: A= 4 mm; B= 200 μm; C-E= 50 μm; D-F-G= 20 μm; H= 10 μm.

Thumbnail

Figure 6
Phylogenetic tree obtained from Bayesian Inference analysis using the sequences of the LSU rDNA obtained from data set of 17 taxa including representatives of Asterinales stricto sensu, lato sensu and the epitype of Asterina mandaquiensis (highlighted in bold). Capnodium coaratum MFLUCC 10006 and Capnodium coffeicola MFLUCC 150206 were used as outgroup.

Thumbnail

Figure 7
Preussia africana (URM 7936). A. Ascoma, asci and ascospores. B-C. Developing asci with young ascospores. D. Asci and ascospores. Scale bars: A: 20 µm; B-D: 10 µm.

Thumbnail

Figure 8
Bayesian inference tree obtained using ITS rDNA sequences of 10 representative species of Preussia performed in MrBayes on XSEDE in the CIPRES science gateway (Miller et al. 2010Miller MA, Pfeiffer W, Schwartz T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE) 14 Nov 2010. New Orleans, Institute of Electrical and Electronics Engineers. p. 1-8.) using the model SYM+G as estimated by the MrModelTest v. 2.3 (Nylander 2004Nylander JAA. 2004. MrModelTest v2. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University.). The new sequence obtained in this study is in bold. Stemphylium botryosum (CBS 116596) was used as an outgroup.

Thumbnail

Figure 9
Diaporthe myracrodruonis (URM 7972 - ex-type culture). A-B. Colony on PDA after 15 days. C-F. Conidiogenous cells. G. Alpha conidia. H. Alpha and beta conidia. Scale bars: 10 µm.

Thumbnail

Figure 10
Bayesian inference tree obtained using ITS rDNA, CaM, tef1-α and TUB2 sequences of species of Diaporthe performed using MrBayes on XSEDE in the CIPRES science gateway with the substitution models GTR+I+G (ITS) and HKY+G (CaM, tef1-α and TUB2). The Maximum likelihood analysis using the model GTR+I+G was conducted in RAxML in CIPRES using the combined dataset. BPP and ML-BS above 0.95 and 70 %, respectively, are shown near nodes. The new species is in bold face. Diaporthella corylina (CBS 121124) was used as an outgroup.

Figure 1 Perenniporia centrali-africana. A-B. Culture on PDA after 10 days (B. reverse); C. Skeletal hyphae. D-F. Chlamydospores-like structures. G-H. Generative hyphae with clamp connection (arrowed). Scale bars: 10 µm.

Figure 2 Phylogenetic tree of the Perenniporia constructed using ITS rDNA sequences, showing the position of P. centrali-africana isolated from watermelon leaves. The sequence obtained in this study is in boldface. Support values are from Bayesian inference and maximum likelihood analyses, respectively.

Figure 3 Serpula similis(URM 89847): A. Fresh basidiomata. B. Hymenial Surface. C. Generative hyphae with clamp connections. D. Yellow basidisopores. Scale bars: a, b=10 cm. c, d= 5 μm. Figure: A.M.S. Soares and R. Alvarenga. The macro- (morphology, color, consistency, size) analyses were performed on both fresh and dehydrated basidiomata. Free-hand sections from hymenium and context were visualized under optical microscopy using 3 % KOH + 1 % phloxine, and Melzer’s reagent (Ryvarden 1991Ryvarden L. 1991. Genera of polypores, nomenclature and taxonomy. Synopsis Fungorum 5: 1-363.) Color designation followed Watling (1969Watling R. 1969. Colour identification chart. Edinburgh, Her Majesty`s Stationery Office.).

Figure 4 Phylogenetic tree inferred from ITS and LSU rDNA gene sequences using Bayesian and maximum likelihood analyses with the GTR + G evolution model retrieved by PartitionFinder v1.1.0 (**Lanfear et al. 2012Lanfear R, Calcott B, Ho SYW, Guindon S. 2012. PartitionFinder: combined selection of partitioning schemes. Molecular Biology and Evolution 29: 1695-1701.). Bayesian and likelihood trees showed the same topology displayed here. Branch lengths reflect estimated number of 0.2 changes per site. Bayesian analysis was performed with MrBayes 3.2.1 software (Ronquist et al. 2012Ronquist F, Teslenko M, Mark P, et al. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 1-4.) for 10 million generations with four Markov chains. Likelihood analysis was performed with the same evolutionary model under 1000 rearrangements using RAxML (Stamatakis 2014**Stamatakis A. 2014. Raxml version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312-1313.). Bootstrap support (BS) values after 1000 rearrangements and posterior probabilities are indicated on tree nodes. Branches with PP > 0.95 and BS from 90 % are considered as strongly supported.

Figure 5 Asterina mandaquiensis (VIC42824, epitype). A. Colonies on leaf surface. B. Colony with open thyriothecia and surface mycelium. C. Thyriothecia opened by a central star-shaped fissure. D. Thyriothecium fringed at margins. E. Branched mycelium with appressoria. F. Alternate to unilateral appressoria. G. Ovoid ascus with immature hyaline ascospores. H. Brown and smooth mature bicelular ascospores. Scale bars: A= 4 mm; B= 200 μm; C-E= 50 μm; D-F-G= 20 μm; H= 10 μm.

Figure 6 Phylogenetic tree obtained from Bayesian Inference analysis using the sequences of the LSU rDNA obtained from data set of 17 taxa including representatives of Asterinales stricto sensu, lato sensu and the epitype of Asterina mandaquiensis (highlighted in bold). Capnodium coaratum MFLUCC 10006 and Capnodium coffeicola MFLUCC 150206 were used as outgroup.

Figure 7 Preussia africana (URM 7936). A. Ascoma, asci and ascospores. B-C. Developing asci with young ascospores. D. Asci and ascospores. Scale bars: A: 20 µm; B-D: 10 µm.

Figure 8 Bayesian inference tree obtained using ITS rDNA sequences of 10 representative species of Preussia performed in MrBayes on XSEDE in the CIPRES science gateway (**Miller et al. 2010Miller MA, Pfeiffer W, Schwartz T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE) 14 Nov 2010. New Orleans, Institute of Electrical and Electronics Engineers. p. 1-8.) using the model SYM+G as estimated by the MrModelTest v. 2.3 (Nylander 2004**Nylander JAA. 2004. MrModelTest v2. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University.). The new sequence obtained in this study is in bold. Stemphylium botryosum (CBS 116596) was used as an outgroup.

Figure 9 Diaporthe myracrodruonis (URM 7972 - ex-type culture). A-B. Colony on PDA after 15 days. C-F. Conidiogenous cells. G. Alpha conidia. H. Alpha and beta conidia. Scale bars: 10 µm.

Figure 10 Bayesian inference tree obtained using ITS rDNA, CaM, tef1-α and TUB2 sequences of species of Diaporthe performed using MrBayes on XSEDE in the CIPRES science gateway with the substitution models GTR+I+G (ITS) and HKY+G (CaM, tef1-α and TUB2). The Maximum likelihood analysis using the model GTR+I+G was conducted in RAxML in CIPRES using the combined dataset. BPP and ML-BS above 0.95 and 70 %, respectively, are shown near nodes. The new species is in bold face. Diaporthella corylina (CBS 121124) was used as an outgroup.

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[1] * Corresponding authors: re.biologicas@gmail.com; adriene_soares@yahoo.com.br; andrefirmino@ufu.br; jadsondpb@gmail.com