The survey is a inventory floristic of Eunotiaceae family from Guaraguaçu river, one of the important lotic ecosystems of Litoranea watershed, State of Paraná, South Brazil. Samples were collected on April and October of 2003 from plankton and periphyton at six localities along the river. Forty species were identified and 16 taxa were registered for first time to State of Paraná: Eunotia anamargaritae,E. auriculata, E. batavica, E. convexa var. convexa f. impressa, E. dacostae,E. denticulata,E. fallax var. groenlandica, E. herzogii,E. meridiana, E. muscicola var. tridentula,E. nymanniana, E. pseudoindica, E. trinacria, Eunotia sp. 2, Eunotia sp. 3 and Eunotia sp. 4.
diatoms; taxonomy; littoral basin; plankton; periphyton