Body Surface Potential Mapping during Ventricular Depolarization in Rats after Acute Exhaustive Exercise

Ivonin, Alexey G.; Smirnova, Svetlana L.; Roshchevskaya, Irina M.

Alexey G. Ivonin

Department of Comparative Cardiology – Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, Syktyvkar – Russian Federation

https://orcid.org/0000-0001-6140-1252

Svetlana L. Smirnova

Department of Comparative Cardiology – Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, Syktyvkar – Russian Federation

Irina M. Roshchevskaya

Department of Comparative Cardiology – Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, Syktyvkar – Russian Federation

Laboratory of Pharmacological Screening – Research Zakusov Institute of Pharmacology, Moscow – Russian Federation

Mailing Address: Alexey G. Ivonin • Department of Comparative Cardiology – Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences – 24 Kommunisticheskaya str. Syktyvkar 167982 – Russian Federation E-mail: alexivonin@mail.ru

Author Contributions

Conception and design of the research, Acquisition of data and Critical revision of the manuscript for important intellectual content: Ivonin AG, Smirnova SL, Roshchevskaya IM; Analysis and interpretation of the data: Ivonin AG, Roshchevskaya IM; Statistical analysis and Writing of the manuscript: Ivonin AG.

Potential Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Figures (5)
Tables (2)

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Figure 1
Positions of electrodes on the rat’s body surface using 64 unipolar torso leads. Four rows of needle electrodes were placed on the ventral, and four rows on the dorsal surface of the body (eight electrodes in each row). When using 32 unipolar torso leads, the number of electrode rows on the ventral and dorsal body surfaces decreased twice.

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Figure 2
Body surface equipotential maps during ventricular depolarization before and after acute exhaustive treadmill exercise in the same rat. The areas of positive (filled) and negative potentials are shown. The signs, plus and minus, show the location of the positive and negative extrema, respectively. Below each map, the time relative to the RII-wave peak and the amplitudes of the positive and negative extrema are shown. Near to each map, ECGII is shown with a time marker (vertical line). The isopotential contour spacing is 0.2 mV. In each map, the left part represents the ventral body surface, and the right represents the dorsal body surface. Pre-Ex: before exhaustive exercise; Post-Ex 5: 5 minutes after exhaustive exercise; Post-Ex 10: 10 minutes after exhaustive exercise.

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Figure 3
Temporal parameters of BSPMs during ventricular depolarization, before and after acute exhaustive treadmill exercise. The time is shown relative to the RII-wave peak. Data are expressed as mean ± standard deviation (n = 20). Pre-Ex: before exhaustive exercise; Post-Ex 5: 5 minutes after exhaustive exercise; Post-Ex 10: 10 minutes after exhaustive exercise. Repeated measures ANOVA and Dunnett’s post-hoc test; *p < 0.05 vs. Pre-Ex.

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Figure 4
Duration of individual phases and total duration of ventricular depolarization on BSPMs before and after acute exhaustive treadmill exercise. Data are expressed as mean ± standard deviation (n = 20). PreEx: before exhaustive exercise; Post-Ex 5: 5 minutes after exhaustive exercise; Post-Ex 10: 10 minutes after exhaustive exercise. Repeated measures ANOVA and Dunnett’s post-hoc test; *p < 0.05 vs. Pre-Ex.

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Figure 5
Maximum amplitudes of positive (A) and negative (B) extrema on BSPMs during ventricular depolarization, and the time when positive and negative extrema reached maximum amplitudes (C and D, respectively) before and after acute exhaustive treadmill exercise. The time is shown relative to the RIIwave peak. Data are expressed as median, first and third quartiles, minimum and maximum (n = 20). PreEx: before exhaustive exercise; Post-Ex 5: 5 minutes after exhaustive exercise; Post-Ex 10: 10 minutes after exhaustive exercise. Friedman test, p > 0.05.

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Table 1
ECGII parameters before and after acute exhaustive treadmill exercise

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Table 2
Amplitudes of BSPM extrema at different time moments during ventricular depolarization before and after acute exhaustive treadmill exercise

	Pre-Ex	Post-Ex 5	Post-Ex 10
HR, bpm	480.3 ± 23.3	484.5 ± 25.2	490.7 ± 28.8
QRS_IIduration, ms	16.0 ± 1.1	15.3 ± 1.2*	15.8 ± 1.7
R_II-wave duration, ms	9.7 ± 1.0	9.1 ± 0.9*	9.4 ± 1.0
S_II-wave duration, ms	6.3 ± 1.4	6.2 ± 1.5	6.3 ± 1.7
R_II-wave amplitude, mV	0.62 ± 0.16	0.65 ± 0.15	0.63 ± 0.14
S_II-wave amplitude, mV	-0.28 ± 0.15	-0.35 ± 0.18*	-0.35 ± 0. 19*
Sum of RII- and SII-wave amplitudes, mV	0.90 ± 0.19	0.99 ± 0.20*	0.98±0.20*

		Time moment, ms
		-5	-4	-3	-2	-1	0	1	2	3	4
Positive extremum, mV	Pre-Ex	0.19 (0.06; 0.31)	0.44 (0.19; 0.54)	0.67 (0.41; 0.81)	0.89 (0.80; 1.09)	1.06 (0.90; 1.20)	0.92 (0.79; 1.16)	0.74 (0.54; 0.91)	0.45 (0.17; 0.79)	0.41 (0.29; 0.63)	0.4 (0.33; 0.52)
	Post-Ex 5	0.14 (0.05; 0.24)	0.34 (0.19; 0.46)	0.57 (0.43; 0.73)	0.88 (0.67; 1.05)	1.02 (0.78; 1.20)	0.95 (0.77; 1.15)	0.76 (0.39; 0.9)	0.33 (0.22; 0.59)	0.44 (0.19; 0.59)	0.34 (0.20; 0.46)
	Post -Ex 10	0.19 (0.06; 0.28)	0.41 (0.19; 0.50)	0.67 (0.44; 0.77)	0.84 (0.56;1.02)	0.95 (0.75; 1.16)	0.95 (0.76; 1.15)	0.61 (0.28; 0.91)	0.39 (0.18; 0.57)	0.41 (0.21; 0.58)	0.4 (0.2; 0.53)
Negative extremum, mV	Pre-Ex	-0.14 (-0.11; -0.21)	-0.28 (-0.16; -0.35)	-0.40 (-0.26; -0.52)	-0.58 (-0.38; -0.64)	-0.64 (-0.51; -0.73)	-0.71 (-0.62; -0.85)	-0.58 (-0.49; -0.74)	-0.42 (-0.29; -0.56)	-0.28 (-0.20; -0.40)	-0.16 (-0.09; -0.27)
	Post -Ex 5	-0.14 (-0.07; -0.19)	-0.26 (-0.18; -0.35)	-0.42 (-0.27; -0.50)	-0.57 (-0.41;-0.67)	-0.65 (-0.58; -0.78)	-0.77 (-0.68; -0.92)*	-0.65 (-0.44; -0.78)	-0.39 (-0.22; -0.44)	-0.21 (-0.14; -0.30)*	-0.08 (-0.05; -0.189)*
	Post -Ex 10	-0.16 (-0.08; -0.20)	-0.29 (-0.17; -0.35)	-0.45 (-0.30; -0.53)	-0.60 (-0.40; -0.69)	-0.66 (-0.55; -0.75)	-0.75 (-0.60; -0.90)	-0.67 (-0.45; -0.73)	-0.39 (-0.20; -0.46)	-0.23 (-0.13; -0.36)	-0.12 (-0.06; -0.23)

[1] Mailing Address: Alexey G. Ivonin • Department of Comparative Cardiology – Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences – 24 Kommunisticheskaya str. Syktyvkar 167982 – Russian Federation E-mail: alexivonin@mail.ru

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Body Surface Potential Mapping during Ventricular Depolarization in Rats after Acute Exhaustive Exercise

Abstract

Background

Objective

Methods

Results

Conclusion