Intestinal microbiome characterization of adult Brazilian men with psoriasis compared to omnivore and vegetarian controls

Polo, Tatiana Cristina Figueira; Lai, Mariana Righetto de Ré; Miot, Luciane Donida Bartoli; Bento, Giovana Fernanda Cosi; Silva, Márcia Guimarães da; Marques, Silvio Alencar; Miot, Hélio Amante

doi:10.1016/j.abd.2022.08.008

Acessibilidade / Reportar erro

Brasil

Anais Brasileiros de Dermatologia

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Article • An. Bras. Dermatol. 98 (5) • Sep-Oct 2023 • https://doi.org/10.1016/j.abd.2022.08.008 copy

Intestinal microbiome characterization of adult Brazilian men with psoriasis compared to omnivore and vegetarian controls^☆ ☆ Study conducted at the Department of Dermatology, Botucatu Faculty of Medicine, Universidade Estadual Paulista, Botucatu, SP, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Background

Psoriasis is a chronic inflammatory disease associated with systemic inflammation and comorbidities. Changes in the composition of the intestinal microbiome are involved in the pathogenesis of inflammatory diseases and metabolic syndrome. Characterizing the intestinal microbiome of patients with psoriasis may be relevant for the understanding of its clinical course and comorbidity prevention.

Objective

To characterize the intestinal microbiome of men with psoriasis compared to omnivore and vegetarian controls (without psoriasis).

Method

Cross-sectional study of 42 adult males: 21 omnivores with psoriasis; and controls: 14 omnivores and 7 vegetarian individuals. The characterization of the intestinal microbiome was performed by metagenomic analysis. Serum levels of lipopolysaccharide-binding protein (LPB) and C-reactive protein (CRP) were evaluated.

Results

The groups differed from each other regarding nutritional aspects and microbiome; individuals with psoriasis had a higher consumption of protein and lower consumption of fibers. Levels of LPB, CRP, and the Firmicutes/Bacteroidetes ratio were higher in the group with psoriasis than in the vegetarian group (p < 0.05). The genera Prevotella, Mogibacterium, Dorea, Bifidobacterium and Coprococcus, differed in the group with psoriasis compared to vegetarians; the genera Mogibacterium, Collinsella and Desulfovibrio differed from omnivores. A microbiome pattern linked to psoriasis (plsPSO) was identified, which was associated with higher LPB levels (rho = 0.39; p = 0.02), and lower dietary fiber intake (rho = −0.71; p < 0.01).

Study limitations

Only adult men were evaluated.

Conclusion

A difference was identified in the intestinal microbiome of adult men with psoriasis when compared to healthy omnivores and vegetarian controls. The identified microbiome pattern was correlated with dietary fiber intake and serum levels of LPB.

Keywords
Diet; Western diet; Gastrointestinal microbiome; Microbiota; Obesity; Psoriasis; Vegetarian diet

	Omnivore controls (n = 14)	Vegetarian controls (n = 7)	Psoriasis	p-Value
Age (years) ^* d p ≤ 0.05 - Compared to vegetarians.	41.6 (11.3)^a a p ≤ 0.05 - Compared to vegetarians.	25.4 (2.5)	51.1 (11.1)^a a p ≤ 0.05 - Compared to vegetarians.	<0.001
BMI (kg/m²) ^* d p ≤ 0.05 - Compared to vegetarians.	31.1 (9.2)^a a p ≤ 0.05 - Compared to vegetarians.	24.4 (2.5)	29.5 (6.2)^a a p ≤ 0.05 - Compared to vegetarians.	<0.001
Energy (cal) ^* d p ≤ 0.05 - Compared to vegetarians.	3.859.4 (1297.5)^a a p ≤ 0.05 - Compared to vegetarians.	2.314.3 (129.9)	3.058.0 (512.0)^a a p ≤ 0.05 - Compared to vegetarians. ,^b b p ≤ 0.05 - Compared to omnivores.	<0.001
Carbohydrates (g) ^* d p ≤ 0.05 - Compared to vegetarians.	539.5 (185.7)^a a p ≤ 0.05 - Compared to vegetarians.	362.2 (22.8)	466.9 (86.8)^a a p ≤ 0.05 - Compared to vegetarians.	<0.001
Proteins (g) ^* d p ≤ 0.05 - Compared to vegetarians.	198.9 (76.0)^a a p ≤ 0.05 - Compared to vegetarians.	58.4 (21.6)	133.4 (39.3)^a a p ≤ 0.05 - Compared to vegetarians. ,^b b p ≤ 0.05 - Compared to omnivores.	<0.001
Lipids (g) ^* d p ≤ 0.05 - Compared to vegetarians.	101.2 (45.5)^a a p ≤ 0.05 - Compared to vegetarians.	72.0 (9.7)	73.9 (23.2)	0.022
Fibers (g) ^* d p ≤ 0.05 - Compared to vegetarians.	21.1 (9.3)^a a p ≤ 0.05 - Compared to vegetarians.	42.4 (7.1)	13.8 (7.4)^a a p ≤ 0.05 - Compared to vegetarians. ,^b b p ≤ 0.05 - Compared to omnivores.	<0.001
LPB (mg/mL) ^* d p ≤ 0.05 - Compared to vegetarians.	6.1 (2.9)	4.8 (1.5)	7.6 (2.9)^a a p ≤ 0.05 - Compared to vegetarians.	0.007
hs-CRPqt (mg/dL) ^* d p ≤ 0.05 - Compared to vegetarians.	2.6 (3.2)	1.0 (0.6)	5.2 (9.2)^a a p ≤ 0.05 - Compared to vegetarians. ,^b b p ≤ 0.05 - Compared to omnivores.	0.003

	Omnivore controls (n = 14)	Vegetarian controls (n = 7)	Psoriasis (n = 21)	p-Value^a a p-value adjusted for age and Body Mass Index.b Mean (standard deviation); c Median (P25-P75).
Relative abundance (10³)^b b p ≤ 0.05 - Compared to omnivores.	62.0 (12.6)	63.7 (13.7)	63.6 (11.0)	0.989
Hill's Diversity^b b p ≤ 0.05 - Compared to omnivores.
q = 0 (richness)	141.2 (53.6)	136.6 (21.1)	138.5 (49.8)	0.353
q = 1 (Shannon)	38.0 (18.0)	33.6 (15.2)	39.4 (18.1)	0.507
q = 3 (Simpson)	13.4 (6.7)	11.8 (7.3)	14.5 (7.5)	0.813
Abundance according to phylum^c
Firmicutes (10³)	40.1 (37.9-46.6)^d d p ≤ 0.05 - Compared to vegetarians.	35.1 (29.6-37.0)	39.1 (33.1-48.8)^d d p ≤ 0.05 - Compared to vegetarians.	0.049
Bacteroidetes (10³)	17.7 (6.5-23.1)	32.0 (10.9-40.4)	13.1 (6.8-23.5)	0.335
F/B ratio	2.5 (1.8-6.0)	1.0 (0.9-3.5)	3.1 (2.2-5.9)^d d p ≤ 0.05 - Compared to vegetarians.	0.045

Sociedade Brasileira de Dermatologia Av. Rio Branco, 39 18. and., 20090-003 Rio de Janeiro RJ, Tel./Fax: +55 21 2253-6747 - Rio de Janeiro - RJ - Brazil
E-mail: revista@sbd.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Corresponding author. E-mail:tatiana.figueira@yahoo.com.br (T.C. Polo).

Brasil

Brasil

Intestinal microbiome characterization of adult Brazilian men with psoriasis compared to omnivore and vegetarian controls☆ ☆ Study conducted at the Department of Dermatology, Botucatu Faculty of Medicine, Universidade Estadual Paulista, Botucatu, SP, Brazil.

Abstract

Background

Objective

Method

Results

Study limitations

Conclusion

Intestinal microbiome characterization of adult Brazilian men with psoriasis compared to omnivore and vegetarian controls^☆ ☆ Study conducted at the Department of Dermatology, Botucatu Faculty of Medicine, Universidade Estadual Paulista, Botucatu, SP, Brazil.