Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.

Keywords:
Collagen; Microscopy, polarization; Skin

Authorship

Polyana Galvão Bernardes Coelho Mailing address: Polyana Galvão Bernardes Coelho. E-mail: polyanagalvao@gmail.com

Department of Veterinary, Universidade Federal de Viçosa (UFV), Viçosa (MG), Brazil.Universidade Federal de ViçosaBrazilViçosa, MG, BrazilDepartment of Veterinary, Universidade Federal de Viçosa (UFV), Viçosa (MG), Brazil.

https://orcid.org/0000-0002-8145-8173

Maria Verônica de Souza

https://orcid.org/0000-0002-2656-3333

Lissandro Gonçalves Conceição

https://orcid.org/0000-0001-7898-8766

Marlene Isabel Vargas Viloria

https://orcid.org/0000-0002-3045-3241

Sirley Adriana Ortiz Bedoya

2 Scholarship holder of the Program Estudantes-Convênio de Pós-Graduação (PEC-PG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) - Brazil.

https://orcid.org/0000-0001-8349-2597

Mailing address: Polyana Galvão Bernardes Coelho. E-mail: polyanagalvao@gmail.com

Conflict of interest: None.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (5)

Thumbnail

Figure 1
Photomicrographs of healthy canine (A, C, E) and equine (B, D, F) skin stained with picrosirius red, with a standardized program for image capture. Skin evaluated without polarized microscopy (A and B) and with polarized light, allowing for the observation of all its constituents (C and D) (x40). Birefringence of hair (green tones) and collagen (E and F) (x100). Ep: epidermis; D: dermis; Gs: sebaceous glands; Gp: sweat glands; *: hairs; arrowhead: arrector pili muscle

Thumbnail

Figure 2
Photomicrographs of dog skin stained with picrosirius red and analyzed in three different ways. Microscopy with non-polarized light (A) and polarized light microscopy with the program QCapture Pro 6 standardized by the authors and with automatic configuration, respectively (B and C) (picrosirius red, x40)

Thumbnail

Figure 3
Photomicrographs of equine (A, C and E) and canine (B, D and F) skin sections during light polarization with the rotation of the filter U-POT. Approximate angle of 60º (A and B), 80º (C and D) and 90º (E and F). During this process, we can observe the gradual change in the background (*) and of the birefringence (arrow) (picrosirius red, x200)

Thumbnail

Figure 4
Photomicrographs showing inversion (fine arrow) and loss of birefringence (thick arrow) of the collagen fiber. Photomicrographs of canine (A and B) and equine (C, D, E and F) skin stained with picrosirius red obtained by polarization in a standardized program (x200). Note the change in the tone of the birefringence of the collagen fibers with a rotation above 90º of the filter U-POT (B, D and F), whereas in the remaining images (A, C and E) the rotation corresponds to 90º

Thumbnail

Figure 5
Folded histologic section in equine skin. Photomicrograph evaluated under polarization with an approximate rotation of 60º (A) and 90º (B) of the filter U-POT. Note the absence of birefringence (thick arrow) (picrosirius red, x200)

Figure 1 Photomicrographs of healthy canine (A, C, E) and equine (B, D, F) skin stained with picrosirius red, with a standardized program for image capture. Skin evaluated without polarized microscopy (A and B) and with polarized light, allowing for the observation of all its constituents (C and D) (x40). Birefringence of hair (green tones) and collagen (E and F) (x100). Ep: epidermis; D: dermis; Gs: sebaceous glands; Gp: sweat glands; *: hairs; arrowhead: arrector pili muscle

Figure 2 Photomicrographs of dog skin stained with picrosirius red and analyzed in three different ways. Microscopy with non-polarized light (A) and polarized light microscopy with the program QCapture Pro 6 standardized by the authors and with automatic configuration, respectively (B and C) (picrosirius red, x40)

Figure 3 Photomicrographs of equine (A, C and E) and canine (B, D and F) skin sections during light polarization with the rotation of the filter U-POT. Approximate angle of 60º (A and B), 80º (C and D) and 90º (E and F). During this process, we can observe the gradual change in the background (*) and of the birefringence (arrow) (picrosirius red, x200)

Figure 4 Photomicrographs showing inversion (fine arrow) and loss of birefringence (thick arrow) of the collagen fiber. Photomicrographs of canine (A and B) and equine (C, D, E and F) skin stained with picrosirius red obtained by polarization in a standardized program (x200). Note the change in the tone of the birefringence of the collagen fibers with a rotation above 90º of the filter U-POT (B, D and F), whereas in the remaining images (A, C and E) the rotation corresponds to 90º

Figure 5 Folded histologic section in equine skin. Photomicrograph evaluated under polarization with an approximate rotation of 60º (A) and 90º (B) of the filter U-POT. Note the absence of birefringence (thick arrow) (picrosirius red, x200)

How to cite

copy

Sociedade Brasileira de Dermatologia Av. Rio Branco, 39 18. and., 20090-003 Rio de Janeiro RJ, Tel./Fax: +55 21 2253-6747 - Rio de Janeiro - RJ - Brazil
E-mail: revista@sbd.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org