Abstract
Background:
Syphilis in its different phases may be a difficult diagnosis in clinical and histopathological grounds.
Objectives:
The present study objectives were to evaluate the detection and tissue distribution of Treponema pallidum in skin lesions of syphilis.
Methods:
A blinded diagnostic accuracy study was performed with immunohistochemistry and Warthin-Starry silver staining in skin samples from patients with syphilis and other diseases. Patients attended two tertiary hospitals between 2000 and 2019. Prevalence ratios (PR) and 95% confidence intervals (95% CI) were calculated for the association between immunohistochemistry positivity and clinical-histopathological variables.
Results:
Thirty-eight patients with syphilis and their 40 biopsy specimens were included in the study. Thirty-six skin samples were used as non-syphilis controls. The Warthin-Starry technique was unable to accurately demonstrate bacteria in all samples. Immunohistochemistry showed spirochetes only in skin samples from patients with syphilis (24/40) with 60% sensitivity (95% CI 44.8-75.2). Specificity was 100% and accuracy, 78.9% (95% CI 69.8-88.1). Most cases had spirochetes in both dermis and epidermis and there was a high bacterial load.
Study limitations:
Correlation between immunohistochemistry and clinical or histopathological characteristics was observed but was limited statistically due to the small sample size.
Conclusions:
Spirochetes were promptly seen in an immunohistochemistry protocol, which can contribute to the diagnosis of syphilis in skin biopsy samples. On the other hand, the Warthin-Starry technique showed to be of no practical value.
KEYWORDS
Diagnosis; Histology; Immunohistochemistry; Syphilis; Treponema pallidum
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