Molecular characterization of Kappa-casein and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds

Bayraktar, M.

doi:10.1590/1678-4162-12497

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Animal Science and Technology and Inspection of Animal Products • Arq. Bras. Med. Vet. Zootec. 74 (01) • Jan-Feb 2022 • https://doi.org/10.1590/1678-4162-12497 linkcopy

Molecular characterization of Kappa-casein and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds

[Caracterização molecular dos genes Kappa-casein e β-lactoglobulina em gado negro anatoliano e raças Holstein]

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

κ-Cn and β-lactoglobulin are important candidate genes associated with milk yield and milk protein content. The present investigation is carried out to determine the polymorphisms status of κ-Cn and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds. PCR-RFLP technique was used to determine Kappa-Casein and β-lactoglobulin polymorphisms in both cattle breeds. The allele frequency of Anatolian Black cattle in terms of κ-Cn and β-lactoglobulin genes were 0.50 (A) 0.50 (B) and 0.20 (A) 0.80 (B) respectively, whereas in Holstein were 0.29 (A) 0.71 (B) and 0.44 (A) 0.56 (B) respectively. The chi-square test showed that each cattle breed was in Hardy-Weinberg equilibrium (P>0.05).

Keywords: κ-Cn; β-lactoglobulin; Anatolian Black; Holstein; polymorphism

Primer code	primer sequences	PCR bp	Sources	PCR conditions	RE
κ-Cn	F/5’-TGTGCTGAGTAGGTATCCTAGTTATGG-3’ R/5’GCGTTGTCTTCTTTGATGTCTCCTTAG-3’	453	Barroso et al., (1998)	95°C 5m, 95°C 10s, 62°C 20s, 72°C 30s, 35 cycles 72°C 5m	HinfI
β-lactoglobulin	F/5’-CGCTCCCCACCCCGTCCTCACC-3’ R/5’-CCCGTCCCCAGTCACCCACAGG-3’	434	Zhao et al., (1999)	94°C 3m, 94°C 30s, 60°C 40s, 72°C 1m, 39 cycles 72°C 10m	HaeIII

Cattle breed	N	Allele Frequency		Genotype frequency			χ²
Cattle breed	N	A	B	AA	AB	BB
Anatolian Black	100	0.50	0.50	0.30	0.40	0.30	2
Holstein	100	0.29	0.71	0.50	0.41	0.09	4.84
Allele and genotype frequencies for β-lactoglobulin gene
Anatolian Black	100	0.20	0.80	0.020	0.36	0.62	0.78
Holstein	100	0.44	0.56	0.26	0.36	0.38	3.63

	κ-CN gene
References	Breed	Allele frequency
Curi et al., (2005)	Canchim	0.77(A)	0.23(B)
Zhang et al. (2006)	Chinese Holstein	0.83(A)	0.17(B)
Rohallah et al. (2007)	Sistani cattle	0.63(A)	0.37(B)
Sulimova et al., (2007)	Kalmyk	0.68(A)	0.32(B)
Azevedo et al. (2008)	Sindhi	0.70(A)	0.30(B)
	Gyr	0.94(A)	0.06(B)
	Guzerat	0.86(A)	0.14(B)
	Nellore	0.82(A)	0.18(B)
Riaz et al. (2012)	Sahiwal	0.69(A)	0.31(B)
	Cholistani	0.90(A)	0.10(B)
	Red Sindhi	0.86(A)	0.14(B)
Farhadi et al. (2014)	Najdi cattle	0.54(A)	0.46(B)
Deb et al. (2014)	Frieswal heifers	0.79(A)	0.21(B)
Djedovic et al. (2015)	Simmental	0.67(A)	0.33(B)
	Crossbreds	0.87(A)	0.13(B)
	Busha	0.67(A)	0.33(B)
Lukač et al., (2015)	Holstein	0.88(A)	0.12(B)
Maletić et al. (2016)	Holstein	0.57(A)	0.43(B)
Maletić et al. (2016)	Busha	0.72(A)	0.28(B)
Ozdemir et al., (2018)	Holstein	0.82(A)	0.18(B)
Tyulkin et al., (2018)	Black Motley	0.77(A)	0.23(B)
Barbosa et al. (2019)	Girolando	0.86(A)	0.14(B)
β-lactoglobulin
References	Breed	Allele frequency
Zhang et al. (2006)	Chinese Holstein	0.27(A)	0.73(B)
Rachagani and Gupta (2008)	Sahiwal	0.17(A)	0.83(B)
Rachagani and Gupta (2008)	Tharparkar	0.39(A)	0.61(B)
Karimi et al. (2009)	Najdi cattle	0.09(A)	0.91(B)
Zaglool et al. (2016)	Holstein	0.36(A)	0.64(B)
Čítek et al. (2019)	Czech	0.47(A)	0.53(B)
Ferreira et al. (2019)	Crossbred	0.40(A)	0.60(B)
Pavlova et al. (2019)	Kholmogory	0.61(A)	0.39(B)
	Simmental Austrian selection	0.42(A)	0.58(B)
	Simmental local selection	0.29(A)	0.71(B)
Barbosa et al. (2019)	Girolando	0.52(A)	0.48(B)

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[TFN1] x²;test of Hardy-Weingberg equilibrium; (P>0.05)