Serum protein concentrations in Wistar rats experimentally infected with Trypanosoma evansiThis study established the electrophoretic profile of serum proteins of Wistar rats experimentally infected with Tripanosoma evansi. For such, 40 rats were allocated into eight groups of five animals. A group was kept as control (G1) and the others (G2 to G8) were intraperitoneally inoculated with 1.0 x 10³ tripomastigote of T. evansi. Blood samples were collected at 5th (G2), 10th (G3), 15th (G4), 30th (G5), 45th (G6), 60th (G7), and 75th (G1 and G8) days after inoculation (DAI). The serum protein concentrations were determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-one distinct proteins were identified, seven of these were identified as acute phase proteins: ceruloplasmin (110KD), hemopexin (83KD), transferrin (75KD), albumin (66KD), antitrypsin (60KD), haptoglobin (44KD), and acid glycoprotein (38KD). The proteins with molecular weights 12KD; 22KD; 25KD; 28KD; 32,5KD; 35KD; 53,5KD; 63KD, and 72KD were found only in infected rats.
rat; protein; electrophoresis; Trypanosoma evansi
