Effects of different extracts of the mushroom <b>Agaricus
                  blazei</b> Murill on the hematologic profile of mice with Ehrlich
               tumor

Verçosa Júnior, D.; Ferraz, V.P.; Duarte, E.R.; Oliveira, N.J. F.; Soto-Blanco, B.; Cassali, G.D.; Melo, M.M.

doi:10.1590/1678-4162-7595

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Veterinary Medicine • Arq. Bras. Med. Vet. Zootec. 67 (3) • May-Jun 2015 • https://doi.org/10.1590/1678-4162-7595 copy

Effects of different extracts of the mushroom Agaricus blazei Murill on the hematologic profile of mice with Ehrlich tumor

Efeitos de diferentes extratos do cogumelo Agaricus blazei Murill sobre o perfil hematológico de camundongos com tumor de Ehrlich

Authorship SCIMAGO INSTITUTIONS RANKINGS

The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage of CD4+ and CD8+ lymphocytes in Ehrlich tumor-bearing mice. The treatment with the ABM extract in water bath and ultrasound at pH 4 resulted in lower lymphocyte counts, regardless of tumor presence, and greater granulocyte values in mice with Ehrlich tumor than in controls. We concluded that different fractions and methods of extraction of A. blazei produced differing blood profiles in mice inoculated with the Ehrlich tumor.

mouse; Ehrlich solid tumor; polysaccharides; hematological profile

Treatments for four experiments with two tumor groups with four treatments
Experiment 1: GI – without tumor
A	Distilled water
B	Total solution at pH 7 extracted in a water bath
C	Polysaccharide solution at pH 7 extracted in a water bath
D	Supernatant solution at pH 7 extracted in a water bath
Experiment 1: GII – with Ehrlich tumor
A	Distilled water
B	Total solution at pH 7 extracted in a water bath
C	Polysaccharide solution at pH 7 extracted in a water bath
D	Supernatant solution at pH 7 extracted in a water bath

Experiment 2: GI – without tumor
E	Distilled water
F	Total solution at pH 4 extracted in a water bath
G	Polysaccharide solution at pH 4 extracted in a water bath
H	Supernatant solution at pH 4 extracted in a water bath
Experiment 2: GII – with Ehrlich tumor
E	Distilled water
F	Total solution at pH 4 extracted in a water bath
G	Polysaccharide solution at pH 4 extracted in a water bath
H	Supernatant solution at pH 4 extracted in a water bath

Experiment 3: GI – without tumor
I	Distilled water
J	Total solution at pH 7 extracted in an ultrasonic bath
K	Polysaccharide solution at pH 7 extracted in an ultrasonic bath
L	Supernatant solution at pH 7 extracted in an ultrasonic bath
Experiment 3: GII – with Ehrlich tumor
I	Distilled water
J	Total solution at pH 7 extracted in an ultrasonic bath
K	Polysaccharide solution at pH 7 extracted in an ultrasonic bath
L	Supernatant solution at pH 7 extracted in an ultrasonic bath

Experiment 4: GI – without tumor
M	Distilled water
N	Total solution at pH 4 extracted in an ultrasonic bath
O	Polysaccharide solution at pH 4 extracted in an ultrasonic bath
P	Supernatant solution at pH extracted in an ultrasonic bath
Experiment 4: GII – with Ehrlich tumor
M	Distilled water
N	Total solution at pH 4 extracted in an ultrasonic bath
O	Polysaccharide solution at pH 4 extracted in an ultrasonic bath
P	Supernatant solution at pH 4 extracted in an ultrasonic bath

Extraction method		Polymerizationdegree
water bath at pH 7	3.151	18
water bath at pH 4	2.348	13
ultrasonic bath at pH 7	1.864	10
ultrasonic bath at pH 4	1.763	10

Treatments			CD4+ lymphocytes %	CD8+ lymphocytes %
Experiment 1: pH 7 water bath extract
GI-A		Distilled water	31.26	10.83
GII-A		Distilled water	31.62	18.12
GII-B		Total solution	32.11	20.24
GII-C		Polysaccharide solution	35.07	24.02
GII-D		Supernatant solution	37.35	18.40

Experiment 2: pH 4 water bath extract
GI-E		Distilled water	31.26	10.80
GII-E		Distilled water	31.62	18.00
GII-F		Total solution	26.79	22.21
GII-G		Polysaccharide solution	27.81	22.08
GII-H		Supernatant solution	29.17	20.31

Experiment 3: pH 7 ultrasonic bath extract
GI-I		Distilled water	31.26	10.83
GII-I		Distilled water	31.62	18.30
GII-J		Total solution	31.73	24.45
GII-K		Polysaccharide solution	28.32	16.94
GII-L		Supernatant solution	31.24	17.99

Experiment 4: pH 4 ultrasonic bath extract
GI-M	Distilled water		31.26	10.87
GII-M	Distilled water		31.62	18.27
GII-N	Total solution		34.05	19.68
GII-O	Polysaccharide solution		30.96	17.76
GII-P	Supernatant solution		28.81	24.37

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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