Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

Pereira, B.A.; Rocha, L.G.P.; Teles, M.C.; Silva, W.E.; Barbosa, J.A.; Rabelo, S.S.; Uchoa, A.S.; Rodrıguez-Gil, J. E.; Pereira, L.J.; Zangeronimo, M.G.

doi:10.1590/1678-4162-10415

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Veterinary Medicine • Arq. Bras. Med. Vet. Zootec. 71 (02) • Mar-Apr 2019 • https://doi.org/10.1590/1678-4162-10415 linkcopy

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

Adição de ácido clorogênico e cafeína durante o processamento do sêmen suíno resfriado

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

Keywords: antioxidant; boar semen storage; metabolic activator; methylxanthine; polyphenol

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[TFN1] * Incubation period differ by F test (P <0.05)

[TFN2] ^a
^b Within each incubation time, means followed by different upper letters in the column and lower case letters in the line differ by the F-test (P <0.05)

Incubation time	Chlorogenic acid	Caffeine		SEM	P =
Incubation time	Chlorogenic acid	without	with	SEM	CA	CH	CH x CA
- Before storage (diluted semen) -
10 minutes	without	81.0 *	82.5 *	1.03	0.23	<0.01	0.24
	with	82.0 *	84.5 *
120 minutes	without	72.5 A	71.0 A
	with	79.0 B	81.0 B
- 24 hours of storage -
10 minutes	without	81.0 *	82.5 *	1.03	0.23	<0.01	0.24
	with	82.0 *	84.5 *
120 minutes	without	72.5 A	71.0 A
	with	79.0 B	81.0 B
- 72 hours of storage -
10 minutes	without	57.5 Aa*	61.5 Ab*	1.00	0.34	<0.01	0.10
	with	67.5 Ba*	70.6 Bb*
120 minutes	without	47.5 A	46.5 A
	with	64.0 B	61.5 B

Incubation time	Chlorogenic acid	Caffeine		SEM	P =
Incubation time	Chlorogenic acid	without	with	SEM	CA	CH	CH x CA
- Before storage (diluted semen) -
10 minutes	without	38.6 Aa*	40.7 b*	0.40	<0.01	<0.01	0.09
	with	40.2 B*	41.2
120 minutes	without	36.5 Aa	38.5 Ab
	with	38.5 Ba	40.8 Bb
- 24 hours of storage -
10 minutes	without	38.4 Aa	40.9 Ab*	0.49	<0.01	<0.01	0.88
	with	41.2 Ba*	43.2 Bb*
120 minutes	without	37.6 A	38.7 A
	with	39.6 B	41.0 B
- 72 hours of storage -
10 minutes	without	35.4 Aa*	37.4 Ab*	0.47	<0.01	<0.01	0.12
	with	37.8 B	39.1 B
120 minutes	without	33.1 Aa	35.5 Ab
	with	36.6 Ba	38.6 Bb

Chlorogenic acid	Caffeine		SEM	P =
Chlorogenic acid	without	with	SEM	CA	CH	CH x CA
- Before storage (diluted semen) -
Without	2.27	2.05	0.10	0.68	0.88	0.55
With	2.10	2.15
- 72 hours of storage -
Without	5.17Aa	5.93Ab	0.15	0.01	<0.01	0.66
With	4.33B	4.88B

Incubation time	Chlorogenic acid	Caffeine		SEM	P =
Incubation time	Chlorogenic acid	without	with	SEM	CA	CH	CH x CA
- Before storage (diluted semen) -
10 minutes	without	92.8	92.7	0.74	0.14	0.22	0.28
	with	93.6	94.3
120 minutes	without	93.2	91.4
	with	93.3	92.3
- 24 hours of storage -
10 minutes	without	89.4 A*	88.6 A*	0.70	0.41	<0.01	0.01
	with	93.1 B*	91.2 B*
120 minutes	without	81.4 Aa	83.7 Ab
	with	90.0 B	89.1 B
- 72 hours of storage -
10 minutes	without	57.5 Aa*	61.5 Ab*	1.00	0.34	<0.01	<0.01
	with	67.0 Ba*	70.5 Bb*
120 minutes	without	47.5 A	46.5 A
	with	64.0 B	61.5 B

Incubation time	Chlorogenic acid	Caffeine		SEM	P =
Incubation time	Chlorogenic acid	without	with	SEM	CA	CH	CH x CA
- Before storage (diluted semen) -
10 minutes	without	97.1 a	96.0 Ab	0.32	0.79	<0.01	<0.01
	with	97.8 *	98.3 B
120 minutes	without	96.3	95.4 A
	with	96.8 b	98.0 Ba
- 24 hours of storage -
10 minutes	without	93.8	95.3 *	0.62	0.07	<0.01	0.04
	with	95.0 *	95.8
120 minutes	without	92.7	91.0 A
	with	92.5 a	94.3 Bb
- 72 hours of storage -
10 minutes	without	89.8 A*	90.4 A*	0.41	0.15	<0.01	0.18
	with	92.3 B*	91.8 B*
120 minutes	without	88.5 Aa	85.8 Ab
	with	90.2 B	90.6 B