Lipopolysaccharide effects on activation and apoptosis of primordial ovarian follicles in heifers

Maffi, A.S.; Rincón, J.A. Alvarado.; Barbosa, A.A.; Weiller, M.A.; Mondadori, R.G.; Gasperin, B.G.; Rovani, M.T.; Cruz, L.A.X.; Freitas, K.C.; Schneider, A.; Corrêa, M.N.; Feijó, J.O.; Pino, F.A.B. Del; Rabassa, V.R.; Marins, L.; Brauner, C.C.

doi:10.1590/1678-4162-12848

Acessibilidade / Reportar erro

Brasil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Veterinary Medicine and Technology and Inspection of Animal Products • Arq. Bras. Med. Vet. Zootec. 75 (03) • May-Jun 2023 • https://doi.org/10.1590/1678-4162-12848 copy

Lipopolysaccharide effects on activation and apoptosis of primordial ovarian follicles in heifers

[Efeitos de lipopolissacarídeos na ativação e apoptose de folículos ovarianos primordiais em novilhas]

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The objective of the present study was to evaluate the effect of lipopolysaccharide (LPS) administration on activation and apoptosis of primordial follicles. There was no difference in the total number of follicles as well as in the different types of follicles. Furthermore, the LPS challenge didn’t modulate the expression of genes related with ovarian reserve (HAM), oocyte survival (Survivin), activation rate (Pten, KIT, KITL1, KITL2, AKT1, SIRT1), and follicular abnormalities. Therefore, the LPS exposure with 24h interval had no effect on activation rate and primordial follicles abnormalities, and also had no effect on expression of anti-apoptotic genes and genes related with ovarian reserve, oocyte survival, activation rate, and primordial follicles abnormalities.

Keywords:
endotoxin; follicle; inflammation; oocyte; reproduction

Gene	Primer sequence 5´ → 3´	NCBI Code	Reference
H2AC6	F: GAGGAGCTGAACAAGCTGTTG	NM_001205596.1	[55]
	R: TTGTGGTGGCTCTCAGTCTTC	NM_001205596.1	[55]
RN18S1	F: CCTTCCGCGAGGATCCATTG	NR_036642.1	[56]
	R: CGCTCCCAAGATCCAACTAC	NR_036642.1	[56]
UXT	F: TGTGGCCCTTGGATGGATGGTT	NM_001037471.2	[57]
	R: GGTTGTCGCTGAGCTGAGCTG	NM_001037471.2	[57]
GAPDH	F: GATTGTCAGCAATGCCTCCT	NM_001034034.2	[56]
	R: GGTCATAAGTCCCTCCACGA	NM_001034034.2	[56]
KIT	F: ATCATGAAGACCTGCTGGGATGCT	NM_001166484.1	[58]
	R: GGGCTGCAGTTTGCTAAGTTGGAA	NM_001166484.1	[58]
KITL1	F: GTGTGATTTCCTCAACATCAAGTCC	NM_174375.2	[58]
	R: TGCTACTGCTGTCATTCCTAAGGG	NM_174375.2	[58]
KITL2	F: AAGGGAAGGCCTCAAATTCCATTGAAGA	NM_174375.2	[58]
	R: AGCAAACCCGATCACAAGAGA	NM_174375.2	[58]
AMH	F: ACACCGGCAAGCTCCTCAT	NM_173890.1	[59]
	R: CACCATGTTTGGGACGTGG	NM_173890.1	[59]
MTOR	F: TCCTTGTCACGAGGCAACAA	XM_002694043.6	This study
	R: GGCGTATCAATTCTTGCAATGA	XM_002694043.6	This study
AKT1	F: GATTCTTCGCCAGCATCGTG	NM_173986.2	[60]
	R: GGCCGTGAACTCCTCATCAA	NM_173986.2	[60]
PTEN	F: GCCACAAAGTGCCTCGTTTACC	NM_001319898.1	[61]
	R: AGAAGGCAACTCTGCCAAACAC	NM_001319898.1	[61]
SIRT1	F: CAACGGTTTCCATTCGTGTG	NM_001192980.3	[62]
	R: GTTCGAGGATCTGTGCCAAT
PIK3R1	F: ACACAGCTGACGGGACCTTT	NM_174575.1	[61]
PIK3R1	R: CCATATTTCCCATCTCGGTGA	NM_174575.1	[61]

Group / Phase	Control	LPS	P-value
Primordial	15872± 3.225 (15.83%)	16557± 6395 (16.07%)	0.92
Transition	23916 ± 3786 (30.92%)	21285 ± 4461 (18.05%)	0.67
Primary	4369 ± 1476 (3.70%)	6235 ± 1498 (5.53%)	0.40
Secondary	1416± 441 (1.07%)	1292 ± 406 (0.96%)	0.84
Abnormal primordial	7308 ± 1874(10.02%)	15042±6939 (18.51%)	0.31
Abnormal transition	13236± 2339 (33.30%)	22245 ± 13755 (28.12%)	0.90
Abnormal primary	3422 ± 977 (3.92%)	11067± 6426 (10.72%)	0.16
Abnormal secondary	1400 ± 206 (1.24%)	2167 ± 595 (2.01%)	0.25

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] Corresponding author: andressamaffi@gmail.com