ABSTRACT
The perfluorocarbon liquids (PFCLs) are a group of compounds with a specific gravity greater than water that are used as an adjunct in the surgical management of complicated vitreoretinal diseases. Thirty-two pigmented rabbits underwent vitrectomy with injection of one of three PFCLs or saline into the vitreous cavity: eight eyes received perfluorotributylamine (PFTA); eight eyes received perfluorodecaline (PFDC); eight eyes received perfluoropolyether (PFPE); the remaining eight eyes, which served as controls, received saline. One hour after placement in the eyes the PFCLs were removed from the vitreous cavity and the eyes were filled with saline. The rabbits were then euthanized and the eyes enucleated and processed for histologic examination. Light microscopy demonstrated no significant difference between eyes with the PfCLs compared with those of control animais. Electron microscopy revealed only minor changes, consisting of a few irregulary shaped defects in the outer segment discs of photoreceptors, in the PFDC group only. These findings suggest that short-term intraoperative use of PFTA, PFPE, and PFDC, at least in this experimental animal model, is not associated with any significant retinal pathology.
Key words:
Experimental; intraoperative; perfluorocarbon liquids; vitrectomy
RESUMO
Perfluorocarbonos líquidos (PFCLs) são compostos com uma gravidade específica maior que a água e utilizados como adjuntos no tratamento cirúrgico de casos complicados de descolamento de retina. Trinta e dois coelhos pigmentados foram submetidos a vitrectomia com injeção de PFCL dentro da cavidade vítrea: oito receberam perfluorotributilamino (PFTA); oito receberam perfluorodecalino (PFDC); oito receberam perfluoropoliéter (PFPE); os restantes oito olhos, que serviram de controle, receberam solução salina. Uma hora depois das injeções foi realizada troca PFCI/solução salina. Os animais foram então sacrificados, os olhos enucleados e processados para exames histológicos. À microscopia óptica nenhuma diferença significativa foi demonstrada entre os olhos com PFCLs comparando com os controles. A microscopia eletrônica revelou somente pequenas alterações, consistindo de alguns defeitos irregulares na forma dos segmentos externos dos fotorreceptores no grupo do PFDC. Estes fatos sugerem que o uso intra-operatório do PFTA, PFPE e PFDC, pelo menos em nosso modelo animal, não é associado com nenhuma patologia retiniana significativa.
Full text available only in PDF format.
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Presented in part at the annual meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Florida, April 30, 1991.
ACKNOWLEDGEMENTS
The authors thank Christine Spee for her skilled technical assistance. The editorial assistance of Ann Dawson is greatly appreciated.
REFERENCES
-
1ARMALY MF: Ocular tolerance to silicones. I. Replacement of aqueous and vitreous by silicone fluids. Arch Ophthalmol 68:390-395, 1962.
-
2LEE P, DONOVAN RH, MUKAI N, SCHEPENS CL, FREEMAN HM: Intravitreous injection of silicone: an experimental study. I. Clinical picture and histology of the eye. Ann Ophthalmol 1(2):15-25, 1969.
-
3MUKAI N, LEE P, SCHEPENS CL: Intravitreous injection of silicone: an experimental study. II. Histochemistry and electron microscopy. Ann Ophthalmol 4:273-287, 1972.
-
4LABELLE'P, OKUN E: Ocular tolerance to liquid silicone: an experimental study. Can J Ophthalmol 7:199-204, 1972.
-
5MUKAI N, LEE P, OGURI M, SCHEPENS CL: A long-term evaluation of silicone retinopathy in monkeys. Can J Ophthalmol 10:391-402, 1975.
-
6OBER RR, BLANKS JC, OGDEN TE, PICKFORD M, MINCKLER OS, RYAN SJ: Experimental retinal tolerance to liquid silicone. Retina 3: 77-85, 1983.
-
7CHAN C, OKUN E: The question of ocular tolerance to intravitreal liquid silicone: long-term analysis. Ophthalmology 93:651-660, 1986.
-
8GONVERS M, HORNUNG JP, DE COURTEN C: The effect of liquid silicone on the rabbit retina: histologic and ultrastructural study. Arch Ophthalmol 104: 1057-1062, 1986.
-
9PAREL JM: Silicone oils: physicochemical properties. In: Retina, Vol. 3, Glaser BM & Michels RG, editors; Ryan SJ, editor-in-chief. St. Louis, CV Mosby, pp. 261-277, 1989.
-
10LEAN JS: Use of silicone oil as an additional technique in vitreoretinal surgery. In: Retina, Vol. 3, Glaser BM & Michaels RG, editors; Ryan SJ, editor-in-chief. St. Louis, CV Mosby, pp. 279-292, 1989.
-
11HAIDT SJ, CLARK LC JR, GINSBERG J: Liquid perfluorocarbon replacement of the eye. ARVO Abstracts. Invest Ophthalmol Vis Sci 22(Suppl.): 233, 1982.
-
12MIYAMOTO K, REFOJO MF, TOLENTINC FI, FOURNIER GA, ALBERT DM: Perfluoroether liquid as a long-term vitreous substitute: An esperimental study. Retina 4:264-268, 1984.
-
13MIYAMOTO K, REFOJO MF, TOLENTINO FI, FOURNIER GA, ALBERT DM: Fluorinated oils as experimental vitreous substitutes. Arch Ophthalmol 104:1053-1056, 1986.
-
14CHANG S, ZIMMERMAN NJ, IWAMOTO T, ORTIZ R, FARIS D: Experimental vitreous replacement with perfluorotributylamine. Am J Ophthalmol 103:29-37, 1987.
-
15HAMMER ME, RINDER DF, HICKS EL, YANG CH, HORNUNG CA: Tolerance of perfluorocarbons, fluorosilicone, and silicone liquids in the vitreous. In: Proliferative vitreoretinopathy (PVR). Freeman HM & Tolentino FI, editors, New York, Springer-Verlag, pp. 156-161, 1988.
-
16TERAUCHI H, OKINAMI S, KOZAKI Z, TANIHARA H, NAGATA M, SEGAWA Y: Experimental study on the effects of a replacement of the vitreous body with perfluorotributylamine on the rabbit eye. Nippon Ganka Gankai Zasshi 93:294-301, 1989.
-
17NABIH M, PEYMAN GA, CLARK LC JR, HOFFMAN RE, MICELI M, ABOUSTEIT M, TAWAKOL M, LIU KR.: Experimental evaluation of perfluorophenathrene as a high specific gravity vitreous substitute: a´preliminary report. Ophthalmic Surg 20:286-293, 1989.
-
18FLORES-AGUILAR M, CRAPOTTA JA, MUNGUIA D, BERGERON-LYNN G, LONG D, WILEY CA, FREEMAN WR: Perfluorooctylbromide (PFOB) as a temporary vitreous substitute. ARVO Abstracts. Invest Ophthalmol Vis Sci 32(Suppl.):1225, 1991.
-
19CHANG S: Low viscosity liquid fluorochemicals in vitreous surgery. Am J Ophthalmol 103:38-43, 1987.
-
20CHANG S, REPPUCCI V, ZIMMERMAN NJ, HEINEMANN MH, COLEMAN DJ: Perfluorocarbon liquids in the management of traumatic retinal detachments. Ophthalmology 96:785-792, 1989.
-
21CHANG S, LINCOFF H, ZIMMERMAN NJ, FUCHS W: Giant retinal tears: Surgical techniques and results using perfluorocarbon liquids. Arch Ophthalmol 107:761-766, 1989.
-
22CHANG S, OZMERT E, ZIMMERMAN NJ: Intraoperative perfluorocarbon liquids in the management of proliferative vitreoretinopathy. Am J Ophthalmol 106:668-674, 1988.
-
23MC CUEN BW II, DE JUAN E JR, LANDERS MB III, MACHEMER R: Silicone oil in vitreoretinal surgery part 2: Results and complications. Retina 5:198-205, 1985.
-
24CASSWELL AG, GREGOR ZJ: Silicone oil removal. I. The effect on the complications of silicone oil. Br J Ophthalmol 71:893-897, 1987.
-
25LEWIS H, BURKE JM, ABRAMS GW, AABERG TM: Persilicone proliferation after vitrectomy for proliferative vitreoretinopathy. Ophthalmology 95:583-591, 1988.
-
26BIRO GP, BLAIS P: Perfluorocarbon blood substitutes. Crit Rev. Oncol Hematol 6:311-374, 1986.
-
27GEYGER RP: Whole animal perfusion with fluorocarbon dispersions. Fed Proc 29: 1758-1763, 1970.
-
28RIESS JG, LE BLANC M: Perfluoro compounds as blood substitutes. Angewandte Chemie 17:621-634, 1978.
Publication Dates
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Publication in this collection
May-Jun 1992