Evaluation of a collagen matrix in a mandible defect in rats submitted to the use of bisphosphonates

Cunha, Vanessa Vasconcelos; Silva, Paulo Goberlânio de Barros; Lemos, José Vitor Mota; Martins, Joyce Ohana Lima; Freitas, Milena Oliveira; Avelar, Rafael Linard

doi:10.1590/s0102-865020200100000005

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Acta Cirúrgica Brasileira

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Original Article, Experimental Surgery • Acta Cir. Bras. 35 (10) • 2020 • https://doi.org/10.1590/s0102-865020200100000005 copy

Evaluation of a collagen matrix in a mandible defect in rats submitted to the use of bisphosphonates ¹ 1 Research performed at School of Dentistry, Centro Universitário Christus (UNICHRISTUS), Fortaleza-CE, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Purpose:

To assess the effect of a collagen matrix (Mucograft^®) on the inflammatory process in a semi-critical experimental defect model in rats treated with bisphosphonates.

Methods:

Eighteen Wistar rats were randomly divided into three groups: saline (CG), alendronate (ALD) 5mg/kg (AG) or zoledronic acid (ZA) 0.2mg/kg (ZG). ALD was administered orally for 10 weeks and ZA was administered intravascularly on days 0, 7 and 14 and 49. On day 42, a 2mm defect was created and filled with Mucograft^® collagen matrix. The contralateral side was filled with a clot (control side). The animals were euthanized 70 days after the beginning of the experiment and the hemimandibles were radiographically and histologically (counting of empty osteocyte lacunae (%), apoptotic (%) and total osteoclasts, neutrophil and mononuclear inflammatory cells) analyzed. The variables were submitted to ANOVA/Bonferroni and t test (parametric data) (p <0.05, GraphPad Prism 5.0).

Results:

Significant bone repair occurred in the groups treated with Mucograft^®. High number of total inflammatory cells and neutrophils cells were showed in AG (p=0.026 and p=0.035) and AZ groups (p=0.005, p=0.034) on the control sides associated with delayed bone repair and the presence of devitalized bone tissue in AG and ZG on the Mucograft^® side.

Conclusion:

Mucograft^® collagen matrix attenuated the inflammatory process in a mandible defect in rats submitted to the use of bisphosphonates (AG and ZG).

Key words:
Collagen; Osteonecrosis; Biphosphonates; Bone and Bones; Rats

		Experimental group
		Saline	AG	ZG	p-V=value ^a a ANOVA/Bonferroni test;
Empty osteocyte lacunae (%)
	Control	13.2±0.5	42.9±4.3 ^* * p <0.05 vs. saline;	46.8±10.7 ^* * p <0.05 vs. saline;	0.025
	Mucograft^®	11.0±2.3	48.1±5.6 ^* * p <0.05 vs. saline;	41.1±5.3 ^* * p <0.05 vs. saline;	0.003
	p-value ^b	0.269	0.540	0.732
Osteoclasts (n)
	Control	2.0±1.5	2.7±1.2	1.8±0.7	0.842
	Mucograft^®	2.0±0.9	2.5±0.5	3.0±1.7	0.829
	p-value ^b	1.000	0.892	0.523
Apoptotic osteoclasts (%)
	Control	0.0±0.0	22.5±13.1	62.5±21.6 ^* * p <0.05 vs. saline;	0.036
	Mucograft^®	0.0±0.0	33.3±20.1	62.6±18.5 ^* * p <0.05 vs. saline;	0.034
	p-value ^b	1.000	0.722	1.000
Neutrophil polymorphonuclear
	Control	0.2±0.1	44.3±12.5 ^* * p <0.05 vs. saline;	41.6±14.0 ^* * p <0.05 vs. saline;	0.004
	Mucograft^®	0.0±0.0	16.7±5.4 ^* * p <0.05 vs. saline;	18.6±1.6 ^* * p <0.05 vs. saline;	<0.001
	p-value ^b	1.000	0.035	0.034
Mononuclear (n)
	Control	0.0±0.0	15.7±8.1	16.5±10.2	0.446
	Mucograft^®	0.0±0.0	14.0±8.0	19.8±5.9	0.071
	p-value ^b	1.000	0.925	0.777
Inflammatory cells (n)
	Control	0.2±0.1	86.7±23.7 ^* * p <0.05 vs. saline;	85.0±17.3 ^* * p <0.05 vs. saline;	<0.001
	Mucograft^®	0.0±0.0	30.7±10.5 ^* * p <0.05 vs. saline;	38.4±5.4 ^* * p <0.05 vs. saline;	<0.001
	p-value ^b	1.000	0.026	0.005

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[1] Correspondence: Rafael Linard Avelar, Rua Adolfo Gurgel, 133, 60140120 Fortaleza – CE Brasil. Tel.: (55 85)99925-8858 rafael.linard@hotmail.com

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Evaluation of a collagen matrix in a mandible defect in rats submitted to the use of bisphosphonates 1 1 Research performed at School of Dentistry, Centro Universitário Christus (UNICHRISTUS), Fortaleza-CE, Brazil.

Abstract

Purpose:

Methods:

Results:

Conclusion:

Evaluation of a collagen matrix in a mandible defect in rats submitted to the use of bisphosphonates ¹ 1 Research performed at School of Dentistry, Centro Universitário Christus (UNICHRISTUS), Fortaleza-CE, Brazil.