The application of Compont gel in chronic obstructive jaundice rats model

90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.

Results:

Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.

Conclusion:

Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.

Key words:
Jaundice Obstructive; Fibrosis; Liver; Rats

Group	AST	ALB	ALP	TB	γ-GT
1 w
Group A	449.00±150.64	36.60±0.90^a	183.67±10.07^a	10.63±4.26^a	26.33±3.22^a
Group B	478.33±182.17^c	33.30±3.30^d	424.55±141.65^d	42.88±14.38^c	70.67±15.14^c
Group C	118.00±18.68	36.73±4.25	50.67±17.89	0.80±0.62	14.67±5.86
2 w
Group A	306.67±63.69	38.83±1.63^a	125.33±54.24^b	1.10±0.70^a	27.00±9.17^a
Group B	506.83±154.12	34.80±3.23	806.25±147.08^d	40.87±14.39^c	93.67±25.15^c
Group C	95.33±13.87	35.67±3.51	99.67±41.76	0.50±0.20	14.00±4.58
3 w
Group A	406.00±98.80	38.90±1.97^a	107.67±2.52^b	0.20±0.10	15.67±3.22
Group B	285.50±131.53	35.42±4.51	337.20±85.57^d	4.80±1.77	38.50±9.03
Group C	103.33±14.05	35.60±4.77	162.33±44.23	0.53±0.31	18.33±6.43
4 w
Group A	318.67±30.09	39.93±3.06^b	136.33±45.79	0.27±0.15	20.33±10.21
Group B	257.83±127.60	31.08±3.01^c	186.91±115.83	0.60±0.42	26.00±13.01
Group C	113.67±17.79	32.03±1.21	108.67±36.69	0.27±0.21	14.33±3.22
5 w
Group A	225.67±51.62	31.90±1.05	108.67±17.01	0.80±0.10	17.00±5.29
Group B	105.75±10.94^d	30.98±4.27	123.00±51.28	0.45±0.30	18.25±5.56
Group C	34.00±5.29	31.20±5.21	99.33±12.90	0.17±0.11	8.00±2.65

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[1] Correspondence:
Shui-ping Yu No. 6 Shuangyong Road Nanning, Guan-xi China spyu2019@163.com

Brasil

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The application of Compont gel in chronic obstructive jaundice rats model1 1 Research performed at Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People’s Republic of China.

Abstract

Purpose:

Methods:

Results:

Conclusion:

The application of Compont gel in chronic obstructive jaundice rats model¹ 1 Research performed at Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People’s Republic of China.