Use of F18 bioglass putty for induced membrane technique in segmental bone defect of the radius in rabbits

Ten female Norfolk at 24 months of age were used. The animals were randomly separated based on postoperative time points: five rabbits at 21 and four at 42 days. A 1-cm segmental bone defect was created in both radii. The bone defects were filled with an F18 bioglass putty.

Results:

Immediate postoperative radiographic examination revealed the biomaterial occupying the segmental bone defect as a well-defined radiopaque structure with a density close to bone tissue. At 21 and 42 days after surgery, a reduction in radiopacity and volume of the biomaterial was observed, with particle dispersion in the bone defect region. Histologically, the induced membrane was verified in all animals, predominantly composed of fibrocollagenous tissue. In addition, chondroid and osteoid matrices undergoing regeneration, a densely vascularized tissue, and a foreign body type reaction composed of macrophages and multinucleated giant cells were seen.

Conclusions:

the F18 bioglass putty caused a foreign body-type inflammatory response with the development of an induced membrane without expansion capacity to perform the second stage of the Masquelet technique.

Key words
Histology; Bone Substitutes; Bone Development; Materials Testing

Authorship

José Ivaldo Siqueira Silva Júnior

Design the study

Technical procedures

Acquisition of data

Critical revision

Final approval the version to be published

PhDs

Universidade Estadual Paulista – School of Veterinary Medicine and Animal Science – Department of Veterinary Surgery and Animal Reproduction – Botucatu (SP), Brazil.Universidade Estadual PaulistaBrazilBotucatu, SP, BrazilUniversidade Estadual Paulista – School of Veterinary Medicine and Animal Science – Department of Veterinary Surgery and Animal Reproduction – Botucatu (SP), Brazil.

https://orcid.org/0000-0002-8758-0647

Sheila Canevese Rahal ^** Corresponding author: sheila.canevese-rahal@unesp.br

Design the study

Critical revision

Final approval the version to be published

PhDs

https://orcid.org/0000-0002-9211-4093

Jennifer Gabriela Figueroa Coris

Technical procedures

Acquisition of data

PhDs

https://orcid.org/0000-0002-1757-5682

Bruna Martins da Silva

Technical procedures

PhDs

https://orcid.org/0000-0002-9944-8643

Felipe Cesar da Silva Brasileiro

Technical procedures

MScs

Universidade Estadual Paulista – School of Veterinary Medicine and Animal Science Department of Veterinary Clinics – Botucatu (SP), Brazil.Universidade Estadual PaulistaBrazilBotucatu, SP, BrazilUniversidade Estadual Paulista – School of Veterinary Medicine and Animal Science Department of Veterinary Clinics – Botucatu (SP), Brazil.

https://orcid.org/0000-0003-3394-991X

Diana Nascimento

Technical procedures

DVM

https://orcid.org/0000-0003-1520-8720

Zara Alves Lacerda

Technical procedures

MScs

https://orcid.org/0000-0002-1042-9690

Jeana Pereira da Silva

Acquisition of data

PhDs

https://orcid.org/0000-0002-2852-6288

Maria Jaqueline Mamprim

Technical procedures

PhDs

https://orcid.org/0000-0002-2238-5584

Marina Trevelin Souza

Design the study

Critical revision

Final approval the version to be published

PhDs

Vetra – Produtos Cerâmicos de Alta Tecnologia – Ribeirão Preto (SP), Brazil.Vetra – Produtos Cerâmicos de Alta TecnologiaBrazilRibeirão Preto, SP, BrazilVetra – Produtos Cerâmicos de Alta Tecnologia – Ribeirão Preto (SP), Brazil.

https://orcid.org/0000-0002-0291-6034

* Corresponding author: sheila.canevese-rahal@unesp.br

Section editor: Natalia Lausada https://orcid.org/0000-0003-1686-6573

Conflict of interest

Nothing to declare.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (5)

Thumbnail

Figure 1
(a) F18 bioglass putty molded to be used in a 1-cm segmental radial defect in the rabbit. (b) Bone defect filled with F18 bioglass putty.

Thumbnail

Figure 2
Mediolateral (a, c) and craniocaudal (b, d) radiographic views of the rabbit forelimbs. Observe well-defined radiopaque structure with a density close to bone tissue in the immediate postoperative (a, b). Note a reduction in radiopacity and volume of the biomaterial at 42 days (c, d) after surgery.

Thumbnail

Figure 3
Photomicrographs of the induced membrane with F18 bioglass putty at 21 days after surgery. (a) Proliferation of intense, disorganized and highly vascularized fibrocollagenous tissue (arrows), and inflammatory infiltrate (star), encompassing vitreous particles (asterisks). HE, 20X. (b) Tissue composition predominantly of disorganized fibrocollagenous tissue, associated with intense mononuclear inflammatory infiltrate (star), focal areas of cartilage (asterisks), and presence of epithelioid cells and multinucleated giant cells (arrow). HE, 20X. (c) Presence of a pronounced chondroid matrix (star) and osteoid matrix (asterisks) in initial organization, fibrocollagenous tissue, and intense inflammatory infiltrate (arrows). HE, 5X.

Thumbnail

Figure 4
Photomicrographs of the induced membrane with F18 bioglass 42 days after surgery. (a) Proliferation of fibrocollagenous tissue with areas with glassy particles of F18 bioglass (asterisks). HE, 20X. (b) Proliferation of disorganized fibrocollagenous tissue and mononuclear inflammatory infiltrate (star) encompassing areas containing F18 bioglass (asterisks). HE, 40X. (c). Intense and well-organized osteoid matrix (stars), and presence of loose fibrocollagenous tissue (asterisks). HE, 20X.

Thumbnail

Figure 5
Photomicrographs of the induced membrane with F18 bioglass putty stained with Masson trichrome at 21 days (a,b) and 42 days after surgery (c,d). (a) Focal areas of collagen fibers stained blue interspersed with fibrous tissue (star), and presence of vitreous particles (asterisks), 20X. (b) Diffuse presence of disorganized collagenous tissue (star), 40X. (c) Areas of chondroid matrix marked diffusely (star), 20X. (d) Proliferation of chondroid matrix and osteoid matrix both well-organized (star), 40X.

Figure 1 (a) F18 bioglass putty molded to be used in a 1-cm segmental radial defect in the rabbit. (b) Bone defect filled with F18 bioglass putty.

Figure 2 Mediolateral (a, c) and craniocaudal (b, d) radiographic views of the rabbit forelimbs. Observe well-defined radiopaque structure with a density close to bone tissue in the immediate postoperative (a, b). Note a reduction in radiopacity and volume of the biomaterial at 42 days (c, d) after surgery.

Figure 3 Photomicrographs of the induced membrane with F18 bioglass putty at 21 days after surgery. (a) Proliferation of intense, disorganized and highly vascularized fibrocollagenous tissue (arrows), and inflammatory infiltrate (star), encompassing vitreous particles (asterisks). HE, 20X. (b) Tissue composition predominantly of disorganized fibrocollagenous tissue, associated with intense mononuclear inflammatory infiltrate (star), focal areas of cartilage (asterisks), and presence of epithelioid cells and multinucleated giant cells (arrow). HE, 20X. (c) Presence of a pronounced chondroid matrix (star) and osteoid matrix (asterisks) in initial organization, fibrocollagenous tissue, and intense inflammatory infiltrate (arrows). HE, 5X.

Figure 4 Photomicrographs of the induced membrane with F18 bioglass 42 days after surgery. (a) Proliferation of fibrocollagenous tissue with areas with glassy particles of F18 bioglass (asterisks). HE, 20X. (b) Proliferation of disorganized fibrocollagenous tissue and mononuclear inflammatory infiltrate (star) encompassing areas containing F18 bioglass (asterisks). HE, 40X. (c). Intense and well-organized osteoid matrix (stars), and presence of loose fibrocollagenous tissue (asterisks). HE, 20X.

Figure 5 Photomicrographs of the induced membrane with F18 bioglass putty stained with Masson trichrome at 21 days (a,b) and 42 days after surgery (c,d). (a) Focal areas of collagen fibers stained blue interspersed with fibrous tissue (star), and presence of vitreous particles (asterisks), 20X. (b) Diffuse presence of disorganized collagenous tissue (star), 40X. (c) Areas of chondroid matrix marked diffusely (star), 20X. (d) Proliferation of chondroid matrix and osteoid matrix both well-organized (star), 40X.

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Use of F18 bioglass putty for induced membrane technique in segmental bone defect of the radius in rabbits

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[1] * Corresponding author: sheila.canevese-rahal@unesp.br