The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions

Özevren, Hüseyin; Deveci, Engin; Tuncer, Mehmet Cudi

doi:10.1590/s0102-865020200040000006

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Acta Cirúrgica Brasileira

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Sumário

ORIGINAL ARTICLE, Experimental Surgery • Acta Cir. Bras. 35 (4) • 2020 • https://doi.org/10.1590/s0102-865020200040000006 copy

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions ¹1. Scheff SW , Price DA , Hicks RR , Baldwin SA , Robinson S , Brackney C . Synaptogenesis in the hippocampal CA1 field following traumatic brain injury . J Neurotrauma . 2005 ; 22 : 719 - 32 . doi: 10.1089/neu.2005.22.719 .

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Purpose

To investigate the role of Rosmarinic acid (RA) in the prevention of traumatic brain injury and the immunohistochemical analysis of IBA-1 and GFAP expressions.

Methods

Healthy male rats were randomly divided into 3 groups consisting of 10 rats. Groups were as follows; control group, traumatic brain injury (TBI) group, and TBI+RA group. After traumatic brain injury, blood samples were taken from the animals and analyzed with various biochemical markers. And then IBA-1 and GFAP expressions were evaluated immunohistochemically.

Results

Significant results were obtained in all biochemical parameters between groups. Immunohistochemical sections showed IBA-1 not only in microglia and macrophage activity but also in degenerative neurons in blood vessel endothelial cells. However, GFAP reaction and post-traumatic rosmarinic acid administration showed positive expression in astrocytes with regular structure around the blood vessel.

Conclusion

Rosmarinic acid in blood vessel endothelial cells showed that preserving the integrity of astrocytic structure in the blood brain barrier may be an important antioxidant.

Rosmarinic Acid; Glial Fibrillary Acidic Protein; Brain Injuries, Traumatic; Rats

	Control	TBI	TBI+RA
MDA (nmol/g)	33.12±2.74	40.32±3.65***	36.29±2.79 ⁺⁺ *
GSH (µmol/g)	1.41±0.31	0.8±0.18***	1.09±0.26 ⁺⁺ **
MPO (U/g)	3.7±0.87	5.82±0.91***	4.73±0.76 ⁺⁺ **
Edema (%)	60.78±5.02	67.73±6.07*	65.49±7.32

Parameter	Groups	n	Mean±SD	Mean Rank	Kruskal-Wallis Test value	Multiple comparisons for groups (p<0.05)
Dilatation in blood vessels	(1) Control group	10	0.40±0.51	6.70	23.839 P=0*	**(2)
	(2) TBI group	10	3.30±0.67	25.30		(1) (3)
	(3) TBI+RA group	10	1.40±0.51	14.50		**(2)
Inflamation	(1) Control group	10	0.40±0.51	6.30	22.640 P=0*	(2) (3)
	(2) TBI group	10	3.20±0.78	24.60		**(1)
	(3) TBI+RA group	10	1.70±0.67	15.60		**(1)
Degeneration in endothelial cells	(1) Control group	10	0.60±0.51	5.50	21.660 P=0*	(2) (3)
	(2) TBI group	10	2.40±0.51	21.00		**(1)
	(3) TBI+RA group	10	2.30±0.48	20.00		**(1)
Apoptosis in microglia	(1) Control group	10	0.30±0.48	5.80	24.426 P=0*	(2) (3)
	(2) TBI group	10	3.10±0.56	24.70		**(1)
	(3) TBI+RA group	10	1.90±0.56	16.00		**(1)
İBA-1 expression	(1) Control group	10	1.80±0.63	8.00	16.899 P=0*	(2) (3)
	(2) TBI group	10	3.20±0.42	22.70		**(1)
	(3) TBI RA group	10	2.50±0.52	15.80		**(2)
GFAP expression	(1) Control group	10	2.80±0.63	15.40	8,427 P=0,015*	(2) (3)
	(2) TBI group	10	3.30±0.67	20.80		**(3)
	(3 TBI+RA group	10	2.30±0.42	10.30		**(2)

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
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[1] Correspondence
: Prof. Engin Deveci. Department of Histology and Embryology. Faculty of Medicine, Dicle University. Phone: +90 412 2488001 Ext. 4443 (Faculty room). devecie32@hotmail.com