<i>Ocimum basilicum</i> L. (basil) presents pro-apoptotic activity in an Ehrlich’s experimental tumor murine model

This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich’s experimental tumor (EET) in mice.

Methods:

Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 10⁶ (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax.

Results:

The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract.

Conclusions:

The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.

Key words
Ocimum basilicum ; Apoptosis; Breast Neoplasms

Authorship

Phelipe Gabriel dos Santos Sant’Ana

Conception and design the study

Acquisition and interpretation of data

Graduate student

Universidade Federal de São João del-Rei – Departamento de Medicina – Laboratório de Patologia Experimental – Divinópolis (MG) – Brazil.Universidade Federal de São João del-ReiBrazilDivinópolis, MG, BrazilUniversidade Federal de São João del-Rei – Departamento de Medicina – Laboratório de Patologia Experimental – Divinópolis (MG) – Brazil.

https://orcid.org/0009-0002-4997-954X

William Gustavo Lima

Acquisition and interpretation of data

Manuscript preparation

Manuscript writing

PhDs

Universidade Federal de Minas Gerais – Faculdade de Farmácia – Belo Horizonte (MG) – Brazil.Universidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais – Faculdade de Farmácia – Belo Horizonte (MG) – Brazil.

https://orcid.org/0000-0001-8946-9363

Gabriela Francine Martins Lopes

Conception and design the study

Acquisition and interpretation of data

PhDs

https://orcid.org/0000-0001-7428-6003

Sabrina Elisa de Oliveira

Conception and design the study

Acquisition and interpretation of data

Universidade Federal de São João del-Rei – Departamento de Ciências Naturais – Laboratório de Patologia Experimental – São João del-Rei (MG) – Brazil.Universidade Federal de São João del-ReiBrazilSão João del-Rei, MG, BrazilUniversidade Federal de São João del-Rei – Departamento de Ciências Naturais – Laboratório de Patologia Experimental – São João del-Rei (MG) – Brazil.

https://orcid.org/0009-0001-3019-4040

Guilherme Augusto Ferreira da Costa

Conception and design the study

Universidade Federal de São João del-Rei – Departamento de Farmácia – Laboratório de Fitoquímica – Divinópolis (MG) – Brazil.Universidade Federal de São João del-ReiBrazilDivinópolis, MG, BrazilUniversidade Federal de São João del-Rei – Departamento de Farmácia – Laboratório de Fitoquímica – Divinópolis (MG) – Brazil.

https://orcid.org/0009-0009-2959-6067

Luciana Alves Rodrigues dos Santos Lima

Conception and design the study

PhDs

https://orcid.org/0000-0002-4130-0304

Elisângela Elduina Ferreira

Manuscript preparation

Manuscript writing

https://orcid.org/0000-0003-1714-1275

Ivan Carlos dos Santos

Manuscript preparation

Manuscript writing

PhDs

Universidade Federal de São João del-Rei – Departamento de Engenharia de Biossistemas – Laboratório de Biomoléculas e Biofármacos – São João del-Rei (MG) – Brazil.Universidade Federal de São João del-ReiBrazilSão João del-Rei, MG, BrazilUniversidade Federal de São João del-Rei – Departamento de Engenharia de Biossistemas – Laboratório de Biomoléculas e Biofármacos – São João del-Rei (MG) – Brazil.

https://orcid.org/0000-0001-8770-0445

Laila Cristina Moreira Damázio

Histopathological examinations

PhDs

Universidade Federal de São João del-Rei – Departamento de Medicina – Laboratório de Reabilitação – São João del-Rei (MG) – Brazil.Universidade Federal de São João del-ReiBrazilSão João del-Rei, MG, BrazilUniversidade Federal de São João del-Rei – Departamento de Medicina – Laboratório de Reabilitação – São João del-Rei (MG) – Brazil.

https://orcid.org/0000-0001-7370-8892

Rosy Iara Maciel Azambuja Ribeiro

Conception and design the study

Acquisition and interpretation of data

Histopathological examinations

PhDs

https://orcid.org/0000-0002-7374-4743

Flávia Carmo Horta Pinto ^** Corresponding author: flaviahorta@ufsj.edu.br

Conception and design the study

Acquisition and interpretation of data

Histopathological examinations

Manuscript preparation

Manuscript writing

Critical revision

PhDs

https://orcid.org/0000-0003-3070-3930

* Corresponding author: flaviahorta@ufsj.edu.br

Section editor: Norbert Nemeth https://orcid.org/0000-0002-1162-3778

Conflict of interest

Nothing to declare.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (2)

Thumbnail

Figure 1
Antitumoral activity of ethanol extract of Ocimum basilicum L. (basil) (EEOb) aerial parts against Ehrlich experimental tumor in vivo. (a) Tumor growth curve in mice footpads for 28 days. (b) Photomicrographs show the subcutaneous tissue of the implantation the Ehrlich tumor stained using the argyrophilic nucleolar organizer regions (AgNOR) technique (1,000X magnification). (c) Number the AgNORs of mice footpads inoculated with EET after 28 days. Control group received daily saline solution. EEOb group received daily basil extract at 100 mg/kg intraperitoneally. Data are mean ± standard deviation (n = 6).

Thumbnail

Figure 2
Immunohistochemistry of (a) proliferating cell nuclear antigen (PCNA), (b) Bax and (c) Bcl-2 in mice footpads inoculated with Ehrlich experimental tumor after 28 days and treated with ethanol extract of Ocimum basilicum L. (basil) (EEOb) aerial parts. Control group received daily saline solution. EEOb group received daily basil extract at 100 mg/kg intraperitoneally. Data are mean ± standard deviation (n = 6).

Figure 1 Antitumoral activity of ethanol extract of Ocimum basilicum L. (basil) (EEOb) aerial parts against Ehrlich experimental tumor in vivo. (a) Tumor growth curve in mice footpads for 28 days. (b) Photomicrographs show the subcutaneous tissue of the implantation the Ehrlich tumor stained using the argyrophilic nucleolar organizer regions (AgNOR) technique (1,000X magnification). (c) Number the AgNORs of mice footpads inoculated with EET after 28 days. Control group received daily saline solution. EEOb group received daily basil extract at 100 mg/kg intraperitoneally. Data are mean ± standard deviation (n = 6).

Source: Elaborated by the authors.*p < 0.05.

Figure 2 Immunohistochemistry of (a) proliferating cell nuclear antigen (PCNA), (b) Bax and (c) Bcl-2 in mice footpads inoculated with Ehrlich experimental tumor after 28 days and treated with ethanol extract of Ocimum basilicum L. (basil) (EEOb) aerial parts. Control group received daily saline solution. EEOb group received daily basil extract at 100 mg/kg intraperitoneally. Data are mean ± standard deviation (n = 6).

Source: Elaborated by the authors. *p < 0.05.

How to cite

copy

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

<i>Ocimum basilicum</i> L. (basil) presents pro-apoptotic activity in an Ehrlich’s experimental tumor murine model

PlumX

Mensagem

[1] * Corresponding author: flaviahorta@ufsj.edu.br