PURPOSE: To verify which are the possible morphological alterations caused by the contamination of a bacterial monoculture in the abdominal cavity of rats, after treatment by draining, washing and the combination of those two processes. METHODS: Fifty adult female rats were distributed in five groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa. 2. Drainage: intraperitoneal injection of P.aeruginosa and drainage of the abdominal cavity, after 6 hours. 3. Washed intraperitoneal injection of P.aeruginosa and washing of the abdominal cavity, after 6 hours. 4. Washed + drainage: intraperitoneal injection of P.aeruginosa plus drainage and washing of the cavity, after 6 hours 5. Normal: evaluation of the normal intestinal wall. After death, blood culture and peritoneal culture were performed. Fragments of jejunum were removed and processed histologically for morphometric measure. RESULTS: There were not statistically significant differences on the intestinal mucosa's thickness between the 5 groups analyzed in the period of 24 hours. From the 40 animals which received the inoculum, 37 evoluted to death in between 5.83 and 28 hours, with 2 animals from group L surviving for a period of 28 hours. CONCLUSION: The acute sepsis did not cause measurable morphological alterations through this methodology in the intestinal wall of these animals.
Mucosa; Morphometry; Rats; Intestin
