This work was carried out in order to study the distribution of S35 in higher plants, represented in this case by coffee (Coffea arabica L., var. Bourbon). Young coffee plants 6 months old were grown in the greenhouse in nutrient solution and received: a) solution of S35 brushed inthe lower surfaces of selected leaves; b) solution of S35 in nutrient solution in order to be absorbed by roots. In (a) procedure the plants were divided in their parts: roots, stms, young leaves and old leaves, and the leaves were divided in petiole, blade and midrib and veins. In (b) procedure, we had three groups of plants for: 1) studies of translocation, like in (a); 2) radioautography; 3) study of leaching of S35 from leaves. On the other hand, in the field experiment, 4 years old coffee plants received S35 that has been sprayed directly on the leaves. The analysis showed a short translocation of S35 in the coffee plant and: 1) the S35 translocation is mainly from old leaves to newer leaves and very few to the stem and roots; 2) from roots the S35 gets to the other parts of the plant to the old leaves; 3) the autoradiografhy of the coffee plant showed the same results obtained with the analysis of radioactivity in the various parts of the plant; 4) S35 is leached from leaves by rainfall.