This paper deals with the variation of Colletotrichum lindemuthianum (Sacc. et Magn.) Scrib., the fungus of bean anthracnose, one of the most serious diseases of bean in Brazil. Nineteen cultures of C. lindemuthianum were isolated from different regions of the State of São Paulo and were tested on the differential varieties Michelite, Dark Red Kidney and Perry Marrow, and on local varieties. The differential varieties were obtained from Cornell and Beltsville. The Hark Red Kidnety variety from Cornell behaved differently than that from Beltsville when inoculated with cultures C-12, C-13 and C-18. It was therefore impossible to say if these cultures belonged to race delta or to a new race not yet reported. For the culture C-3 and C-15 the Dark Red Kidney variety behaved similary, independent of its origin. Therefore these cultures belong to race alfa. For the other cultures the race is unknown. Local varieties were for the most part susceptible to all isolates tested. Some were variable in their reaction to isolates of the alfa race. Variety 54 was resistant to 18 isolates and variety 131 was resistant to all isolates in a single trial.