The present paper deals with studies related to pollination of Cucurbita pepo L. in the State of S. Paulo, Brazil. The following results were obtained: 1.° - The ratio of female flowers to male flowers openig at the same day was 1:17,7. 2.º - The opening of the female flowers and observed to occur before the sun rises. Closing begins at 9 A.M. and is finished by 11 A. M. 3.° - With respect to pollinizating insects, Trigona (Trigona) ruficrus Latr.) and Apis, mellifera L. constitute the most important species. 4.° - Due to the phenomenon of parthenocarpy no frutification was observed. 5.º - The sugar concentration of the nectar was observed to average 39,7% and 40,6%. 6.º - The pollination of Cucurbita pepo L. is only by insects.