Some aspects of SO4² absorption by excised roots of "Sorghum bicolor (L.) Moench" namely, inibition effects of NaF, KCN, 2,4- DNP and factors such as aeration, solution pH and temperature on the rate of absorption were studied. The excised roots showed multiphasic mecanism of SO4²- absorption. The relative absorption rates, presented by three varieties, were in decreasing order: Tey-101, Contibrasil-101 and Dekalb-E-57. Inhibition effects on the absorption of SO4²- by excised roots were shown in the case of the three ihibitors studied hereof-. Aeration and solution temperature are important factors on SO4²- absorption. At the external solutio n concentration of Na,2SO(4)10-³M the best fitted equation to the experimental values was shown to be: umoles/g dry matter = 1,639- 1,585 exp. (-0,006618t)