Slipes of the varieties Fernão Dias, Formosa and Calabreza were grew in pots containing pure quartz sand. Several times a day all the plants were irrigated with Hoagland and Arnon's solution of 30 days. After, that period, groups of plants (5) were irrigated with nutrient solution lacking one nutrient (-N,-P,-K,-Ca,-Mg,-S and -B). Soon as the deficiences symptoms become visible the plants were harvested and analysed for the macronutrients and boron. The plants showed deficiencies symptoms in the following order: nitrogen, magnesium, calcium, phosphorus, potassium and boron. No sulphur deficiencies symptoms were observed. The tissue analysis of the leaves of plants showing deficiencies symptoms or not were: