CORRELATION OF LEPTIN AND ADIPONECTIN RECEPTOR EXPRESSION WITH CLINICOPATHOLOGICAL PARAMETERS IN COLORECTAL CARCINOMA - A CROSS-SECTIONAL PROSPECTIVE STUDY

PARMESH, Priyanka; DINESH, Udupi Shastri; KHANDAGALE, Ajay S; BAPU, Anil Bargale; SADASHIV, Roshni; REDDY, Pradnya

doi:10.1590/S0004-2803.24612024-016

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Sumário

ORIGINAL ARTICLE/CLINICAL GASTROENTEROLOGY • Arq. Gastroenterol. 61 • 2024 • https://doi.org/10.1590/S0004-2803.24612024-016 copy

CORRELATION OF LEPTIN AND ADIPONECTIN RECEPTOR EXPRESSION WITH CLINICOPATHOLOGICAL PARAMETERS IN COLORECTAL CARCINOMA - A CROSS-SECTIONAL PROSPECTIVE STUDY

Status de expressão do receptor de leptina e adiponectina no carcinoma colorretal: A cross-estudo prospectivo seccional

Authorship SCIMAGO INSTITUTIONS RANKINGS

HIGHLIGHTS

•Radical CRC specimens were subjected for histopathological examination. Immunohistochemistry (IHC) was done for the leptin and adiponectin receptors on paraffin embedded tissue sections. The tissue IHC expression was analyzed and was correlated with various clinicopathological parameters.

•The results concluded that positive expression of leptin was associated with lymph node spread and advanced stage of CRC. Negative expression of adiponectin was associated with metastatic spreadof CRC.

ABSTRACT

Background:

Colorectal carcinoma (CRC) is one of the common carcinomas with a rising incidence of metastasis due to its advanced stage of presentation. The existing biomarkers such as CEA (Carcinoembryonic antigen) etc., for prognosis, have low sensitivity and specificity. Hence a need for a newer definitive biomarker. Obesity is the leading cause of CRC. Leptin and adiponectin secreted by adipose tissue have been studied as potential biomarkers in the field of CRC. The present study helps to understand the association of leptin and adiponectin receptors with clinicopathological parameters.

Objective:

To correlate the various clinicopathological parameters with the tissue expression of leptin and adiponectin receptors in CRC.

Methods:

It is a cross-sectional prospective study conducted at a tertiary care hospital. Formalin fixed paraffin blocks of all radical resection CRC cases were collected and immunohistochemistry (IHC)was carried out on tumor tissue for leptin and adiponectin receptor. Tumor characteristics and clinical parameters were collected from the hospital medical records. Pearson’s correlation coefficient test was used.

Results:

Immunohistochemistry was performed on 60 cases of CRC. Significant positive correlation of leptin was observed with size, lymph node metastasis, advanced stage, and grade of tumor (P<0.05). A significant correlation between adiponectin receptor and CRC was observed concerning age, stage, lymph node metastasis, distant metastasis and grade of tumor.

Conclusion:

Positive expression of leptin and negative expression of adiponectin receptors in CRC helps to predict the risk of metastasis.

Keywords:
Colorectal carcinoma; leptin; adiponectin receptor; immunohistochemistry

Percentage of positive cells x Intensity of Staining			IRS classification
0 = no positive cells	0 = no reaction	0-1 = negative	0 = negative
1=<10% of positive cells	1 = mild reaction	2-3 = mild	1 = positive, weak expression
2=10-50% positive cells	2 = moderate reaction	4-8= moderate	2 = positive, mild expression
3=51-80% positive cells	3 = strong reaction	9-12= strongly positive	3 = positive, strong expression
4=>80% positive cells

Parameters		Number of cases and percentage	Leptin			Adiponectin receptor
			Negative	Positive	P-value	Negative	Positive	P-value
Age	<50 years	21 (35.5%)	11 (52.3%)	9 (42.8%)	Not significant	16 (76.1%)	05 (23.8%)	Significant
								P<0.05
	>50 years	39 (64.5%)	30 (76.9%)	9 (23.07%)		23 (58.3%)	16 (41.02%)
BMI	>25 Overweight	18 (30%)	11 (61.1%)	7 (38.1%)	Not significant	13 (72.2%)	5 (27.7%)	Not significant
	<25 Normal	42 (70%)	30 (71.4%)	12 (28.5%)		28 (66.6%)	14 (33.3%)
Grade	Well	46 (76.6%)	31 (67.4%)	15 (32.6%)	Significant	29 (63%)	17 (37%)	Significant
					P<0.05			P<0.05
	Moderate	9 (15%)	7 (77.8%)	2 (22.2%)		7 (77.8%)	2 (22.2%)
	Poor	5 (8.3%)	2 (40%)	3 (60%)		4 (80%)	1 (20%)
Tumor extension	In situ	1 (1.6%)	0	1 (100%)	Not significant	1 (100)	0 (0)	Not Significant
	T1	3 (5%)	2 (66%)	1 (25%)		1 (33%)	2 (66.7%)
	T2	11 (18.35%)	8 (72%)	3 (30%)		9 (81%)	2 (18.2%)
	T3	37 (61.6%)	24 (64.9%)	13 (35%)		22 (59.5%)	15 (45%)
	T4	8 (13.35%)	6 (75%)	2 (25%)		7 (87.5%)	11 (12.5%)
Lymph Node deposits	Positive	19 (46.3%)	16 (66.7%)	8 (33.3%)	Significant	13 (54.2%)	11 (45.8%)	Significant
					P<0.05			P<0.05
Distant metastasis	Negative	22 (53.7%)	24 (66.7%)	12 (33.3%)		27 (75.0%)	9 (25%)
	Mx	30 (73.2%)	30 (71.4%)	12 (28.6%)	Not Significant	26 (61.9%)	16 (38.1%)	Significant
								P<0.05
	M0	8 (19.5%)	9 (64.3%)	5 (35.7%)		11 (78.1%)	3 (21.4%)
	M1	3 (7.3%)	1 (25.%)	3 (75%)		3 (75%)	1 (25%)
TNM stage	0	1 (2.4%)	0	2 (100%)	Significant	1 (50%)	1 (50%)	Significant
					P<0.05			P<0.05
	I	4 (9.8%)	0	4 (36%)		8 (72.7%)	3 (27.3%)
	II	16 (39%)	5 (55.6%)	4 (21%)		13 (68.4%)	6 (31.6%)
	III	17 (41.5%)	4 (44.4%)	7 (30%)		14 (60.9%)	09 (39.1%)
	IV	3(7.3%)	0	3 (60.1%)		4 (80.9%)	1 (20%)

	Leptin	Adiponectin receptor
Negative expression	40 (66.6%)	40 (66.6%)
Positive expression	20 (33.3%)	20 (33.3%)

Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. Rua Dr. Seng, 320, 01331-020 São Paulo - SP Brasil, Tel./Fax: +55 11 3147-6227 - São Paulo - SP - Brazil
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[1] Corresponding author: Priyanka Parmesh. E-mail: drpriyankamahesh@gmail.com