ABSTRACT
The objective of this work was to evaluate and compare the microbial diversity of a soil treated with sewage sludge (BAR 1N) with the same soil without treatment (control). The use of industrial and domestic sewer sludge as organic fertilizer in agricultural soils is currently considered a promising alternative for the final destination of these residues. Molecular studies using the analysis of the gene 16S rRNA provide relevant information in regard to studies of microbial ecology, since it is believed that only 10% of these microorganisms can be cultivated. The sequences were submitted to analysis of nucleotide similarity, based on data in GenBank, in order to be identified and classified. Following phylogram analysis, a high number of nonidentified microorganisms were observed in the investigated soils. The results demonstrated that the outstanding bacterial phyla were Acidobacteria and Proteobacteria. Phylogenetic analyses revealed differences in both soils, based on the bacterial diversity index, showing that the test soil had more diversity when compared to the BAR 1N soil.
KEY WORDS
Biossolid; microbial ecology; metagenomic
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