ABSTRACT
Nucleopolyhedrovirus isolate from infected Bombyx mori larvae (BmNPV) in Paraná State was used to inoculate healthy fifth-instar larvae. Samples of adipose tissue were removed from the experimental animals at different times, and processed for light microscopy by routine techniques. The samples included both inoculated and non-inoculated larvae (control). Azan staining of sections of infected tissue revealed in the inoculated material different stages in the pathology, indicating the asynchronous replication of the BmNPV. The infection of adipose cells could be detected as early as at the fourth day after inoculation. Initially, it is characterized by nuclear hypertrophy and the presence of virogenic stroma, where the virions are produced. In a second stage, the virions appear occluded into protein crystals, the polyhedra. In a more advanced stage of viral replication, the cells undergo lysis and liberate a great number of polyhedra into the general cavity of the insect body. The control material did not present any signs of infection.
KEY WORDS:
Bombyx mori; adipose tissue; Nucleopolyhedrovirus; pathology.
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