ABSTRACT
The present work had for objective compare the performance of two methods of decontamination in mycobacteria isolation from feces. One of the protocols used the method of Petroff - basic method, using the solution of sodium hidroxid (NaOH) 4%, the other used Löwenstein-Jensen modified method - acid method with sulfuric acid solution (H2SO4) 4%. Thirty females hamsters were inoculated orally with 108 cfu/animal of Mycobacterium avium and your feces were examinated 192 differents experimental moments. Each sample was submitted to the basic and acid method. Mycobacteria was isolated in 62 (32.3%) chances in the basic method and in 56 (29.2%) in the acid method. The “kappa” value was 0.6 demonstrating moderate agreement between them. The Petroff method can be utilized for the decontamination of feces and it is important to test others concentrations than 4% of the sulfuric acid solution in the fetching of the maximum decontamination of the sample and minor delay of the viability and capacity of multiplication of the mycobacteria.
KEY WORDS:
Mycobacterium avium; Petroff method decontamination; bacteriology.
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