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EVALUATION OF CUCUMBER MOSAIC VIRUS PARTICLE CONCENTRATION BY TRANSMISSION ELECTRON MICROSCOPY IN DIFFERENT HOSTS

ABSTRACT

The Cucumber mosaic virus (CMV) is one of the most important plant virus in the world, with a large host range and great number of insect vectors and strains. The best results for diagnosis and characterization are obtained when the plant source of the virus has a satisfactory concentration. In order to evaluate the virus concentration in different hosts and periods of inoculation, electron microscopy of transmission was used the counting of particles. Nicotiana debneyi showed a higher particle concentration than Datura stramonium and Chenopodium amaranticolor.

KEY WORDS:
Cucumovirus; Bromoviridae; Serology; CMV.

Instituto Biológico Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana - São Paulo - SP, 04014-002 - São Paulo - SP - Brazil
E-mail: arquivos@biologico.sp.gov.br